Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,683,424	A→G	E181E (GAA→GAG)	rstB →	sensory histidine kinase RstB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,683,424	0	A	G	100.0%	138.7 / NA	39	E181E (GAA→GAG)	rstB	sensory histidine kinase RstB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

TGGATATCGCCCTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAACGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACAC  >  NC_000913/1683291‑1683557                                                                                                                                      |                                                                                                                                      tGGATATCGCCCTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATc                                                                                                                                 >  2:351215/1‑140 (MQ=255)          cccTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTtgat                                                                                                                        >  1:479624/1‑140 (MQ=255)           ccTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTtgatg                                                                                                                       <  1:324241/140‑1 (MQ=255)           ccTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTtgatg                                                                                                                       <  1:733100/140‑1 (MQ=255)                    ttttATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGa                                                                                                              >  2:68043/1‑140 (MQ=255)                     tttATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAg                                                                                                             >  2:777472/1‑140 (MQ=255)                                      cGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCg                                                                                            >  2:820973/1‑140 (MQ=255)                                                   tttATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCa                                                                               >  2:579477/1‑140 (MQ=255)                                                   tttATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCa                                                                               >  2:543874/1‑140 (MQ=255)                                                        cTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATgg                                                                          <  1:867061/140‑1 (MQ=255)                                                        cTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATgg                                                                          <  1:913213/140‑1 (MQ=255)                                                            aTGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGa                                                                      <  1:820973/140‑1 (MQ=255)                                                                 tCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGa                                                                      >  1:345026/1‑135 (MQ=255)                                                                 tCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGa                                                                      <  2:345026/135‑1 (MQ=255)                                                                 tCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAata                                                                 >  1:153779/1‑140 (MQ=255)                                                                 tCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAata                                                                 >  2:297219/1‑140 (MQ=255)                                                                     cACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCaa                                                             >  2:711393/1‑140 (MQ=255)                                                                     cACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCaa                                                             >  2:431654/1‑140 (MQ=255)                                                                      aCTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGa                                                                      <  2:297847/130‑1 (MQ=255)                                                                      aCTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGa                                                                      >  1:297847/1‑130 (MQ=255)                                                                                tatGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTg                                                  >  2:306552/1‑140 (MQ=255)                                                                                  tGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGcc                                                >  1:141141/1‑140 (MQ=255)                                                                                    ttAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAg                                              >  1:589625/1‑140 (MQ=255)                                                                                          cTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCaaaaa                                        <  1:777472/140‑1 (MQ=255)                                                                                           tGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCaaaaaa                                       <  1:68043/140‑1 (MQ=255)                                                                                            ggAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAAc                                      >  1:768480/1‑140 (MQ=255)                                                                                             gAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGc                                                 >  1:802586/1‑128 (MQ=255)                                                                                             gAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGc                                                 <  2:802586/128‑1 (MQ=255)                                                                                             gAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACa                                     >  1:480014/1‑140 (MQ=255)                                                                                             gAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACa                                     >  2:64060/1‑140 (MQ=255)                                                                                             gAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAG‑AAAAAACAg                                    >  2:26638/1‑140 (MQ=255)                                                                                                 cagcGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCtt                                 <  1:351215/140‑1 (MQ=255)                                                                                                 cagcGGCGCAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCtt                                 <  1:711393/140‑1 (MQ=255)                                                                                                         cAACGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACgg                         <  2:141141/140‑1 (MQ=255)                                                                                                            cGATTTGGCGATGGGCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTAt                      <  1:306552/140‑1 (MQ=255)                                                                                                                        gggCATCTCAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACt          >  1:611616/1‑140 (MQ=255)                                                                                                                             tctcAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGaa     <  2:153779/140‑1 (MQ=255)                                                                                                                             tctcAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGaa     <  2:480014/140‑1 (MQ=255)                                                                                                                                cAATGAGCGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAacac  <  1:543874/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TGGATATCGCCCTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCACTGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAACGTATCCACTTTGATGAGGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACAC  >  NC_000913/1683291‑1683557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑