Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,731,215	A→G	H710R (CAT→CGT)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,731,215	0	A	G	100.0%	110.9 / NA	31	H710R (CAT→CGT)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

GGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGA  >  NC_000913/1731085‑1731346                                                                                                                                   |                                                                                                                                    gggTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTg                                                                                                                            >  1:377248/1‑140 (MQ=255)    ttgattgaGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGc                                                                                                                         >  1:246924/1‑140 (MQ=255)     tgattgaGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCg                                                                                                                        >  1:185389/1‑140 (MQ=255)      gattgaGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCgg                                                                                                                       >  1:398146/1‑140 (MQ=255)         tgaGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTgg                                                                                                                    <  2:377248/140‑1 (MQ=255)                       aCGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGa                                                                                                      <  1:318093/140‑1 (MQ=255)                              gggCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATg                                                                                               <  2:285092/140‑1 (MQ=255)                                              aGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGa                                                                               >  1:208848/1‑140 (MQ=255)                                              aGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGa                                                                               >  1:42294/1‑140 (MQ=255)                                              aGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGa                                                                               >  1:592578/1‑140 (MQ=255)                                                     aTCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCAGGCGAATACATGCGCTATGGGGCGCTGACGCGTCg                                                                        <  2:840720/140‑1 (MQ=255)                                                        tGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCggtt                                                                     <  2:208848/140‑2 (MQ=255)                                                        tGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCggtg                                                                     <  2:42294/140‑1 (MQ=255)                                                        tGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCggtg                                                                     <  2:592578/140‑1 (MQ=255)                                                                      tGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGtgatga                                                       <  1:854153/140‑1 (MQ=255)                                                                      tGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGtgatga                                                       <  2:185389/140‑1 (MQ=255)                                                                         cGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACgg                                                    <  2:398146/140‑1 (MQ=255)                                                                                aaaTTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCAttgtt                                             <  2:246924/140‑1 (MQ=255)                                                                                aaaTTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCAttgtt                                             >  1:427440/1‑140 (MQ=255)                                                                                             gCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGt                                       <  2:309539/133‑1 (MQ=255)                                                                                             gCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGt                                       >  1:309539/1‑133 (MQ=255)                                                                                              cGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGa                               <  2:427440/140‑1 (MQ=255)                                                                                                        tGCACTCTCCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGt                     >  2:79957/1‑140 (MQ=255)                                                                                                         gCACTCTCCCTATGGAAGACGGCTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTa                    <  1:649354/140‑1 (MQ=255)                                                                                                               tcCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGc              >  2:102786/1‑140 (MQ=255)                                                                                                               tcCCTATGGAAGACGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGc              >  1:59289/1‑140 (MQ=255)                                                                                                                           aCGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGa  >  2:365788/1‑140 (MQ=255)                                                                                                                           aCGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGa  >  2:544461/1‑140 (MQ=255)                                                                                                                           aCGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGa  <  2:59289/140‑1 (MQ=255)                                                                                                                           aCGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGa  >  1:509080/1‑140 (MQ=255)                                                                                                                           aCGGGTGCGTGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGa  >  1:186693/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                    GGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTGGTCGCCAGTGATGACGGCATTGTTGCACGTATTCCTGACACCGATGGTAAATTGCCCGATGCCGCGA  >  NC_000913/1731085‑1731346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑