Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,833,212	T→C	V262A (GTC→GCC)	xthA →	exodeoxyribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,833,212	0	T	C	100.0%	177.6 / NA	50	V262A (GTC→GCC)	xthA	exodeoxyribonuclease III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/28);  total (22/28)

AAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGT  >  NC_000913/1833078‑1833346                                                                                                                                       |                                                                                                                                       aaaaGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAACCCGTCCGATCACGCCCCCGCCTggg                                                                                                                                   <  1:289370/140‑1 (MQ=255) aaaaGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTggg                                                                                                                                   <  1:703540/140‑1 (MQ=255) aaaaGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTggg                                                                                                                                   >  1:421580/1‑140 (MQ=255)                 aaCCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTa                                                                                                                   >  1:232046/1‑140 (MQ=255)                     gTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAAttt                                                                                                               <  2:610612/140‑1 (MQ=255)                      tGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTa                                                                                                              >  1:560973/1‑140 (MQ=255)                         tCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTagca                                                                                                           <  2:519227/140‑1 (MQ=255)                          cTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTagcag                                                                                                          <  1:346676/140‑1 (MQ=255)                             cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGctc                                                                                                       >  1:770854/1‑140 (MQ=255)                              gcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGctct                                                                                                      <  1:342385/140‑1 (MQ=255)                                aTCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTcc                                                                                                    <  2:459949/140‑1 (MQ=255)                                     ccTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCt                                                                                               <  2:847717/140‑1 (MQ=255)                                     ccTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCt                                                                                               <  2:512794/140‑1 (MQ=255)                                            ctCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTg                                                                                        >  1:276254/1‑140 (MQ=255)                                               gccagccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGt                                                                                     <  2:560973/140‑1 (MQ=255)                                                   gccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAgg                                                                                 >  1:857231/1‑140 (MQ=255)                                                   gccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAgg                                                                                 >  2:720228/1‑140 (MQ=255)                                                       aCCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAAGCGTCCGATCACGCCCCCTCCTGGGCGAGCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGaa                                                                             >  2:820730/1‑140 (MQ=255)                                                                  aaTGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAgg                                                                                 <  2:187799/125‑1 (MQ=255)                                                                  aaTGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAgg                                                                                 >  1:187799/1‑125 (MQ=255)                                                                   aTGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAACCCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTgaga                                                                 <  2:436214/140‑1 (MQ=255)                                                                   aTGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCt                                                                      <  1:135249/135‑1 (MQ=255)                                                                   aTGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCt                                                                      >  2:135249/1‑135 (MQ=255)                                                                       tGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGaaaaa                                                             >  1:484194/1‑140 (MQ=255)                                                                         cGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATc                                                           >  2:147573/1‑140 (MQ=255)                                                                            aGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATcaac                                                        >  1:576180/1‑140 (MQ=255)                                                                              aaaCCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATcaacaa                                                      >  2:179485/1‑140 (MQ=255)                                                                                aCCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAAc                                                    >  2:161127/1‑140 (MQ=255)                                                                                 ccGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACt                                                   <  1:239282/140‑1 (MQ=255)                                                                                   ggCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGt                                                 <  1:720228/140‑1 (MQ=255)                                                                                   ggCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGt                                                 <  1:809047/140‑1 (MQ=255)                                                                                          aCTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAAt                                          <  1:89383/140‑1 (MQ=255)                                                                                            tttGAAATCCGCAGCATGGAAAAACCGTCCGTTCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGa                                        <  2:103313/138‑1 (MQ=255)                                                                                            tATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGa                                        >  1:803715/1‑140 (MQ=255)                                                                                                  aTCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATttgtt                                  <  2:770854/140‑1 (MQ=255)                                                                                                     cGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATttgtt                                  >  2:736733/1‑137 (MQ=255)                                                                                                     cGCAGCATGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATttgtt                                  <  1:736733/137‑1 (MQ=255)                                                                                                      gcagcaTGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcct                              >  2:452246/1‑140 (MQ=255)                                                                                                        agcaTGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcctgc                            <  2:803715/140‑1 (MQ=255)                                                                                                          caTGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCg                          >  1:195419/1‑140 (MQ=255)                                                                                                          caTGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCg                          <  2:305567/140‑1 (MQ=255)                                                                                                            tGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc                        <  1:147573/140‑1 (MQ=255)                                                                                                            tGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc                        <  1:452246/140‑1 (MQ=255)                                                                                                            tGGAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc                        <  2:484194/140‑1 (MQ=255)                                                                                                              gAAAAACCGTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCt                      <  2:232046/140‑1 (MQ=255)                                                                                                                      gTCCGATCACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTtata              >  2:254843/1‑140 (MQ=255)                                                                                                                             cACGCCCCCGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGtctc       <  1:254843/140‑1 (MQ=255)                                                                                                                              aCGCCCCCGCCTAGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc                        >  1:803714/1‑122 (MQ=255)                                                                                                                              aCGCCCCCGCCTAGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc                        <  2:803714/122‑1 (MQ=255)                                                                                                                                  ccccGCCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGt  >  1:794328/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       AAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGT  >  NC_000913/1833078‑1833346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑