Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,679	T→C	L1581P (CTG→CCG)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,679	0	T	C	100.0%	217.6 / NA	60	L1581P (CTG→CCG)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

TACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGC  >  NC_000913/2049542‑2049814                                                                                                                                          |                                                                                                                                        tACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGc                                                                                                                                       >  2:351692/1‑140 (MQ=255)         cAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACa                                                                                                                               <  2:537087/140‑1 (MQ=255)              tCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGAc                                                                                                                          >  1:893891/1‑140 (MQ=255)                     cTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGa                                                                                                                   <  1:105672/140‑1 (MQ=255)                            tAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCa                                                                                                            <  2:792626/140‑1 (MQ=255)                                  tGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGt                                                                                                      >  2:286824/1‑140 (MQ=255)                                    aGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTa                                                                                                    >  1:225556/1‑140 (MQ=255)                                    aGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTa                                                                                                    >  1:438056/1‑140 (MQ=255)                                         ttGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCaa                                                                                               >  2:754103/1‑140 (MQ=255)                                          tggtggTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCa                                                                                                >  1:76986/1‑138 (MQ=255)                                          tggtggTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCa                                                                                                <  2:76986/138‑1 (MQ=255)                                          tggtggTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAAt                                                                                              >  2:585372/1‑140 (MQ=255)                                          tggtggTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAAt                                                                                              >  2:159829/1‑140 (MQ=255)                                           ggtggtGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATg                                                                                             >  2:79847/1‑140 (MQ=255)                                              ggtgAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAgg                                                                                          <  1:22226/140‑1 (MQ=255)                                              ggtgAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAgg                                                                                          >  2:33390/1‑140 (MQ=255)                                                tgAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCg                                                                                        >  1:625980/1‑140 (MQ=255)                                                 gAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCgg                                                                                       <  2:260862/140‑1 (MQ=255)                                                  aaCTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGt                                                                                      <  2:893891/140‑1 (MQ=255)                                                  aaCTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGt                                                                                      <  1:486349/140‑1 (MQ=255)                                                      ttATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACa                                                                                  >  2:270231/1‑140 (MQ=255)                                                       tATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACAt                                                                                 >  2:692659/1‑140 (MQ=255)                                                       tATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACAt                                                                                 >  1:132542/1‑140 (MQ=255)                                                         ttGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTc                                                                               <  1:506203/140‑1 (MQ=255)                                                            cGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACg                                                                            <  2:345731/140‑1 (MQ=255)                                                            cGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGAGACATTCACg                                                                            >  1:856630/1‑140 (MQ=255)                                                                tACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTAc                                                                        <  1:534951/140‑1 (MQ=255)                                                                   gCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCgg                                                                     <  2:438056/140‑1 (MQ=255)                                                                   gCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCgg                                                                     <  1:692659/140‑1 (MQ=255)                                                                      cACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAg                                                                  >  1:352289/1‑140 (MQ=255)                                                                         tGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGAtg                                                               <  1:159829/140‑1 (MQ=255)                                                                           cgcAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGAtgtg                                                             >  2:370955/1‑140 (MQ=255)                                                                           cgcAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGAGACATTCACGCTACCCGCAGAtggg                                                             >  2:726351/1‑138 (MQ=255)                                                                            gcAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGa                                                            >  1:426252/1‑140 (MQ=255)                                                                                 ttAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCa                                                       <  2:141271/140‑1 (MQ=255)                                                                                   aaTCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCGACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAg                                                     >  2:818256/1‑140 (MQ=255)                                                                                   aaTCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAg                                                     <  2:172235/140‑1 (MQ=255)                                                                                          aCGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTAct                                              >  1:301319/1‑140 (MQ=255)                                                                                          aCGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTAACGCTACCCGCAGATGTTAGCGCCAGCTTTAcc                                              >  2:329048/1‑139 (MQ=255)                                                                                                ccGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGa                                        >  2:826008/1‑140 (MQ=255)                                                                                                   aGGACAA‑TTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAg                                    >  2:861235/1‑140 (MQ=255)                                                                                                     gACAATTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAgg                                   >  1:482694/1‑140 (MQ=255)                                                                                                         aTTTTATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGt                               <  2:625980/140‑1 (MQ=255)                                                                                                          ttttATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGtt                              >  1:497675/1‑140 (MQ=255)                                                                                                           tttATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTc                             <  1:585372/140‑1 (MQ=255)                                                                                                           tttATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTc                             <  1:826008/140‑1 (MQ=255)                                                                                                           tttATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTc                             <  1:329048/140‑1 (MQ=255)                                                                                                           tttATCGCTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTc                             <  1:33390/140‑1 (MQ=255)                                                                                                                  cTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCa                        >  2:505142/1‑138 (MQ=255)                                                                                                                  cTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCa                        <  1:505142/138‑1 (MQ=255)                                                                                                                  cTAATAACGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCAtt                      >  2:370731/1‑140 (MQ=255)                                                                                                                   taataaCGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTa                     <  1:320258/140‑1 (MQ=255)                                                                                                                    aataaCGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGGTTCGCCATTAc                    >  2:514145/1‑140 (MQ=255)                                                                                                                    aacaaCGTCGGGATGACCAGGGCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTAc                    >  2:649704/4‑140 (MQ=255)                                                                                                                     ataaCGTCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACt                   >  2:250927/1‑140 (MQ=255)                                                                                                                           tCGGGATGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATc             <  2:225556/140‑1 (MQ=255)                                                                                                                                aTGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACgg        <  2:132542/140‑1 (MQ=255)                                                                                                                                 tGACCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTAGGACAAGGCGGTTCCGCCATTACTGATATCAACGGc       <  1:286824/140‑1 (MQ=255)                                                                                                                                   aCCAGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCaa     >  2:710339/1‑140 (MQ=255)                                                                                                                                      aGGCCGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGc  <  1:370731/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGC  >  NC_000913/2049542‑2049814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑