Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,129,520	T→C	N44S (AAC→AGC)	wcaE ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,129,520	0	T	C	100.0%	162.9 / NA	46	N44S (AAC→AGC)	wcaE	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/27);  total (19/27)

GAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTC  >  NC_000913/2129386‑2129654                                                                                                                                       |                                                                                                                                       gaAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGaa                                                                                                                                   <  1:340827/140‑1 (MQ=255)     caacGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGc                                                                                                                               >  2:120872/1‑140 (MQ=255)     caacGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGc                                                                                                                               <  1:530418/140‑1 (MQ=255)       acGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCa                                                                                                                             <  2:488831/140‑1 (MQ=255)       acGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCa                                                                                                                             <  1:310142/140‑1 (MQ=255)        cgcgAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCAt                                                                                                                            >  2:259624/1‑140 (MQ=255)         gcgAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATc                                                                                                                           <  2:506349/140‑1 (MQ=255)         gcgAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATc                                                                                                                           <  2:710811/140‑1 (MQ=255)          cgAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCg                                                                                                                          >  1:204515/1‑140 (MQ=255)          cgAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCg                                                                                                                          >  1:1875/1‑140 (MQ=255)                            tCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAg                                                                                                        >  2:667434/1‑140 (MQ=255)                            tCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAg                                                                                                        >  1:626187/1‑140 (MQ=255)                             cGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGc                                                                                                       >  2:738256/1‑140 (MQ=255)                             cGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATAGACAACAATCCATTCGAAGc                                                                                                       >  2:497809/1‑140 (MQ=255)                                aaTGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGa                                                                                                    <  2:253614/140‑1 (MQ=255)                                aaTGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGa                                                                                                    <  2:204515/140‑1 (MQ=255)                                aaTGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGa                                                                                                    <  1:738256/140‑1 (MQ=255)                                aaTGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGa                                                                                                    <  2:819972/140‑1 (MQ=255)                                aaTGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGa                                                                                                    <  2:854585/140‑1 (MQ=255)                                aaTGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATAGACAACAATCCATTCGAAGCTGa                                                                                                    <  1:497809/140‑1 (MQ=255)                                          ttCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCAc                                                                                          <  1:120872/140‑1 (MQ=255)                                               ggCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTgcg                                                                                     >  1:806279/1‑140 (MQ=255)                                                   tcgtAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAg                                                                                 >  2:120771/1‑140 (MQ=255)                                                   tcgtAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAg                                                                                 <  1:223585/140‑1 (MQ=255)                                                               ttATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGa                                                                     >  2:562872/1‑140 (MQ=255)                                                               ttATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGa                                                                     >  1:899931/1‑140 (MQ=255)                                                                 aTCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAgg                                                                   >  2:515194/1‑140 (MQ=255)                                                                   cTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCa                                                                 <  1:60961/140‑1 (MQ=255)                                                                   cTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCa                                                                 <  1:562872/140‑1 (MQ=255)                                                                       cTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCAtgtg                                                             >  2:531916/1‑140 (MQ=255)                                                                        tCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATgtgt                                                            <  1:259624/140‑1 (MQ=255)                                                                               cAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACt                                                     <  1:15888/140‑1 (MQ=255)                                                                               cAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACt                                                     <  2:1875/140‑1 (MQ=255)                                                                                     gTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCt                                               <  2:899931/140‑1 (MQ=255)                                                                                      tAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCtt                                              >  2:98174/1‑140 (MQ=255)                                                                                        ggTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCg                                            >  2:778512/1‑140 (MQ=255)                                                                                                   ccATTGAGATTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGaaa                                 >  1:751464/1‑140 (MQ=255)                                                                                                         agctttcCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGAc                           <  2:420031/137‑1 (MQ=255)                                                                                                         agaTTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGAc                           <  1:778512/140‑1 (MQ=255)                                                                                                         agaTTTTCCAGATACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGAc                           <  1:799566/140‑1 (MQ=255)                                                                                                                     tACTCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCt               >  1:789369/1‑140 (MQ=255)                                                                                                                        tCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATg                 <  1:161222/135‑1 (MQ=255)                                                                                                                        tCACGGGTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATg                 >  2:161222/1‑135 (MQ=255)                                                                                                                            gggTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAAc        <  1:280793/140‑1 (MQ=255)                                                                                                                             ggTGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACa       <  1:531916/140‑1 (MQ=255)                                                                                                                               tGCCGTCGCTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATg     <  2:626187/140‑1 (MQ=255)                                                                                                                                  cgtcgcTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttc  <  2:751464/140‑1 (MQ=255)                                                                                                                                  cgtcgcTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttc  <  2:789369/140‑1 (MQ=255)                                                                                                                                  cgtcgcTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttc  <  2:274713/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTAGATACCGTTATCTGGCTCGCTGACAAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTC  >  NC_000913/2129386‑2129654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑