Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,165	T→C	T48A (ACC→GCC)	yegR ←	uncharacterized protein YegR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,165	0	T	C	100.0%	273.7 / NA	75	T48A (ACC→GCC)	yegR	uncharacterized protein YegR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/38);  total (37/38)

AATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGTGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGATATAGCAGCTATCTTT  >  NC_000913/2168029‑2168301                                                                                                                                          |                                                                                                                                         aaTCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCgta                                                                                                                                       <  1:117484/140‑1 (MQ=255)   tCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCgtagt                                                                                                                                     >  1:712460/1‑140 (MQ=255)     cTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTAt                                                                                                                                   <  1:740794/140‑1 (MQ=255)        cacCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTgg                                                                                                                                >  2:745328/1‑140 (MQ=255)         acCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTgg                                                                                                                                >  1:115137/1‑140 (MQ=255)          ccTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGtt                                                                                                                              >  2:816384/1‑140 (MQ=255)            ttCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTAt                                                                                                                            <  2:879814/140‑1 (MQ=255)            ttCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTAt                                                                                                                            <  1:482175/140‑1 (MQ=255)            ttCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTAt                                                                                                                            <  2:578704/140‑1 (MQ=255)             tCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCgta                                                                                                                                       <  2:503391/128‑1 (MQ=255)             tCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCgta                                                                                                                                       >  1:503391/1‑128 (MQ=255)             tCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTAt                                                                                                                            >  2:441689/1‑139 (MQ=255)             tCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTAt                                                                                                                            <  1:441689/139‑1 (MQ=255)                  cTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGtt                                                                                                                      >  2:311598/1‑140 (MQ=255)                      aGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACg                                                                                                                  <  1:157088/140‑1 (MQ=255)                        cTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCt                                                                                                                <  1:816384/140‑1 (MQ=255)                        cTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCt                                                                                                                >  1:299096/1‑140 (MQ=255)                           aaCTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCt                                                                                                                >  2:209627/1‑137 (MQ=255)                           aaCTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCt                                                                                                                <  1:209627/137‑1 (MQ=255)                                tttGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTa                                                                                                        <  2:910239/140‑1 (MQ=255)                                  tGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACt                                                                                                      >  1:382025/1‑140 (MQ=255)                                       ttttttGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTtat                                                                                                 >  1:480965/1‑140 (MQ=255)                                          tttGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATg                                                                                              <  1:545854/140‑1 (MQ=255)                                          tttGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATg                                                                                              >  1:128442/1‑140 (MQ=255)                                                        cacaGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTa                                                                                <  2:382025/140‑1 (MQ=255)                                                        cacaGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTa                                                                                >  2:842474/1‑140 (MQ=255)                                                         acaGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTAc                                                                               <  2:508239/140‑1 (MQ=255)                                                             aaaTGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGAt                                                                           >  2:227903/1‑140 (MQ=255)                                                             aaaTGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGAt                                                                           >  1:272714/1‑140 (MQ=255)                                                             aaaTGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGAt                                                                           >  2:542632/1‑140 (MQ=255)                                                             aaaTGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGAt                                                                           >  2:515859/1‑140 (MQ=255)                                                                  aGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTg                                                                      >  2:569799/1‑140 (MQ=255)                                                                     tGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGtct                                                                   <  2:638721/140‑1 (MQ=255)                                                                     tGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGtct                                                                   <  2:128442/140‑1 (MQ=255)                                                                     tGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGtct                                                                   >  2:632667/1‑140 (MQ=255)                                                                     tGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGtct                                                                   <  1:311598/140‑1 (MQ=255)                                                                     tGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGtca                                                                   <  1:745328/140‑2 (MQ=255)                                                                       catcatTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCtca                                                                 >  1:70749/1‑140 (MQ=255)                                                                       catcatTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCtca                                                                 <  2:480965/140‑1 (MQ=255)                                                                         tcatTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACtt                                                                       <  1:15033/132‑1 (MQ=255)                                                                         tcatTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACtt                                                                       >  2:15033/1‑132 (MQ=255)                                                                         tcatTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCtcatc                                                               >  2:150277/1‑140 (MQ=255)                                                                            ttttttACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCAtt                                                            >  2:19990/1‑140 (MQ=255)                                                                               tttACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATg                                                         <  1:227903/140‑1 (MQ=255)                                                                               tttACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATg                                                         <  2:299096/140‑1 (MQ=255)                                                                                ttACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgc                                                        <  1:851675/140‑1 (MQ=255)                                                                                ttACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgc                                                        <  2:449285/140‑1 (MQ=255)                                                                                 tACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgca                                                       <  1:873791/140‑1 (MQ=255)                                                                                    tataTTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacag                                                    <  1:542632/140‑1 (MQ=255)                                                                                      tatTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacagca                                                  <  1:150277/140‑1 (MQ=255)                                                                                      tatTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacagca                                                  <  1:19990/140‑1 (MQ=255)                                                                                      tatTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacagca                                                  >  1:889813/1‑140 (MQ=255)                                                                                        ttttCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacagcaca                                                <  2:893195/140‑1 (MQ=255)                                                                                              gCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGa                                          >  1:11882/1‑140 (MQ=255)                                                                                              gCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGa                                          >  1:228539/1‑140 (MQ=255)                                                                                               cACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAAccctgt                                         >  2:750232/1‑136 (MQ=255)                                                                                                aCCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACa                                        >  1:127900/1‑140 (MQ=255)                                                                                                 ccAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacag                                                    <  2:314318/127‑1 (MQ=255)                                                                                                 ccAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATgcacag                                                    >  1:314318/1‑127 (MQ=255)                                                                                                       atTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATaaa                                 <  1:632667/140‑1 (MQ=255)                                                                                                           tA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATa                             <  2:557940/140‑1 (MQ=255)                                                                                                               cccTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACta                          <  2:115137/140‑1 (MQ=255)                                                                                                                 cTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACataat                                    <  1:436394/128‑1 (MQ=255)                                                                                                                 cTGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACataat                                    >  2:436394/1‑128 (MQ=255)                                                                                                                  tGGCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACtaatt                       <  2:127900/140‑1 (MQ=255)                                                                                                                   ggCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACtaatta                      >  1:181411/1‑140 (MQ=255)                                                                                                                    gCTAAAGAAGCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACtaattaa                     >  1:527629/1‑140 (MQ=255)                                                                                                                        aagaagCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGGATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGat                 >  2:877998/1‑140 (MQ=255)                                                                                                                         agaagCTTCGGCTTCGGCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGata                <  2:134777/140‑1 (MQ=255)                                                                                                                              cttcggcttcggCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATa                             >  1:189287/1‑122 (MQ=255)                                                                                                                              cttcggcttcggCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATa                             <  2:189287/122‑1 (MQ=255)                                                                                                                              cttcggcttcggCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGATATagca           >  1:657141/1‑140 (MQ=255)                                                                                                                              cttcggcttcggCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGATATagca           >  1:696730/1‑140 (MQ=255)                                                                                                                                tcggcttcggCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGATATagcagc         >  1:692392/1‑140 (MQ=255)                                                                                                                                       cggCGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGATATAGCAGCTATCttt  <  2:913697/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTA‑CCCCTGGCTAAAGAAGCTTCGGCTTCGGTGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTGTCTCATCATTATGCACAGCACAACCAGACATAATAAAAATACTAATTAATGATATAGCAGCTATCTTT  >  NC_000913/2168029‑2168301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑