Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,184,736	C→G	G189A (GGT→GCT)	thiM ←	hydroxyethylthiazole kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,184,736	0	C	G	100.0%	157.7 / NA	44	G189A (GGT→GCT)	thiM	hydroxyethylthiazole kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (29/15);  total (29/15)

CGCGTTCTCCGGCTTGTTTCATCCAGTGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCACCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCA  >  NC_000913/2184599‑2184871                                                                                                                                           |                                                                                                                                        cgcgTTCTCCGGCTTGTTTCATCCAGTGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATcagca                                                                                                                                       >  1:234392/1‑140 (MQ=255) cgcgTTCTCCGGCTTGTTTCATCCAGTGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATcagca                                                                                                                                       >  2:25218/1‑140 (MQ=255) cgcgTTCTCCGGCTTGTTTCATCCAGTGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATcagca                                                                                                                                       >  1:514974/1‑140 (MQ=255) cgcgTTCTCCGGCTTGTTTCATCCAGTGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATcagca                                                                                                                                       >  1:394623/1‑140 (MQ=255)   ctttCTCCGTTTTTTTTCATCCAGGGACGGGAGGTTGCTACTTTTTCCGGCTTCCCGCCTGGTACCGCACAGCAGGCAGCAAAAC‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTCGGTCATTAACGGATCagcacc                                                                                                                                     <  2:650049/138‑1 (MQ=255)                           tGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTcc                                                                                                             >  2:461676/1‑140 (MQ=255)                           tGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTcc                                                                                                             >  1:800643/1‑140 (MQ=255)                               gggaaGATGCGCTTTTTCCCGGCGTATCGCCGGGAAACCCAAAGCAGGCAGCAAAAC‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCg                                                                                                         <  2:570273/136‑1 (MQ=255)                               gggCAGATGCGCCTTTTCCCAGCGTCCCCCGTGAAACCGCACGGCAGGAAGC‑ACAACCCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCg                                                                                                         <  1:414062/139‑1 (MQ=255)                                     aTGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACa                                                                                                   >  1:570273/1‑140 (MQ=255)                                     aTGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACa                                                                                                   >  2:606244/1‑140 (MQ=255)                                     aTGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACa                                                                                                   >  2:592632/1‑140 (MQ=255)                                     aTGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACa                                                                                                   >  1:73809/1‑140 (MQ=255)                                             ttccccAGCGTCTCCCCTGGAAACGCACGGCAGGCAGCAACAC‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCAt                                                                                           <  2:43521/136‑1 (MQ=255)                                               ttCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCAtct                                                                                         >  1:43521/1‑140 (MQ=255)                                                     cGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAg                                                                                   >  1:916486/1‑140 (MQ=255)                                                            ccTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCac                                                                            >  2:485174/1‑140 (MQ=255)                                                               ggTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCacgac                                                                         >  2:654789/1‑140 (MQ=255)                                                                    cGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTg                                                                    >  2:692077/1‑140 (MQ=255)                                                                      cacaGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTgcg                                                                  >  2:347961/1‑140 (MQ=255)                                                                          gcaggcagCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAg                                                              >  1:675986/1‑140 (MQ=255)                                                                          gcaggcagCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAg                                                              >  2:640440/1‑140 (MQ=255)                                                                          gcaggcagCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAg                                                              >  2:1324/1‑140 (MQ=255)                                                                              gcggCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTc                                                          <  1:25218/137‑1 (MQ=255)                                                                                     cAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGcc                                                   <  1:606244/140‑1 (MQ=255)                                                                                     cAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGcc                                                   <  1:485174/140‑1 (MQ=255)                                                                                      cagccgccgATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGcc                                                   <  2:800643/137‑1 (MQ=255)                                                                                       a‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAg                                                 >  1:311861/1‑140 (MQ=255)                                                                                        cccgccgATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAg                                                 <  2:565029/139‑1 (MQ=255)                                                                                                   tGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAg                                      >  2:898101/1‑140 (MQ=255)                                                                                                   tGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAg                                      >  1:912255/1‑140 (MQ=255)                                                                                                      acaGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCgg                                   >  1:888069/1‑140 (MQ=255)                                                                                                        aGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGt                                 <  2:234392/140‑1 (MQ=255)                                                                                                        aGCCAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGt                                 <  1:692077/140‑1 (MQ=255)                                                                                                          ccAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTAt                               >  2:321160/1‑140 (MQ=255)                                                                                                           cAGTTCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATc                              <  1:749226/140‑1 (MQ=255)                                                                                                              ttCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATcgcg                           >  2:714471/1‑140 (MQ=255)                                                                                                               tCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACTATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGt                          >  2:640538/1‑140 (MQ=255)                                                                                                               tCCTACCACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGt                          <  2:394623/140‑1 (MQ=255)                                                                                                                    ccACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCt                     >  1:149254/1‑140 (MQ=255)                                                                                                                    ccACTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCt                     >  1:2960/1‑140 (MQ=255)                                                                                                                      aCTTTGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTgc                   <  1:640440/140‑1 (MQ=255)                                                                                                                          tGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTgcggcg               >  1:460336/1‑140 (MQ=255)                                                                                                                          tGGTCATTAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTgcggcg               >  1:316386/1‑140 (MQ=255)                                                                                                                                ttAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTg         >  1:773059/1‑140 (MQ=255)                                                                                                                                ttAACGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTg         <  2:2960/140‑1 (MQ=255)                                                                                                                                  aaCGGATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGt       >  1:548802/1‑140 (MQ=255)                                                                                                                                     ggATCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTACAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATc    <  1:461676/140‑1 (MQ=255)                                                                                                                                       aTCAGCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCa  >  2:500112/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        CGCGTTCTCCGGCTTGTTTCATCCAGTGACAGGCAGATGCGACATTTTCCAGCGTATCGCCTGGTAACGCACAGCAGGCAGCGACAA‑CCGCCGATAATGCACAGCCAGTTCCTACCACTTTGGTCATTAACGGATCACCACCGTGAATACCAATGATACGATGTCCATCGGTAACATAATCCATCTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCA  >  NC_000913/2184599‑2184871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑