Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	186,485	G→A	A722A (GCC→GCT)	glnD ←	PII uridylyltransferase/uridylyl removing enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	186,485	0	G	A	100.0%	134.9 / NA	41	A722A (GCC→GCT)	glnD	PII uridylyltransferase/uridylyl removing enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

ATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGAT  >  NC_000913/186350‑186618                                                                                                                                        |                                                                                                                                      aTGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCaa                                                                                                                                   <  1:269473/140‑1 (MQ=255)  tGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCaaa                                                                                                                                  >  1:676637/1‑140 (MQ=255)       aTCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGAt                                                                                                                             >  2:618360/1‑140 (MQ=255)           gCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAgg                                                                                                                         >  2:200293/1‑140 (MQ=255)                   cGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCg                                                                                                                 >  1:711395/1‑140 (MQ=255)                     ggTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCggg                                                                                                               <  1:618360/140‑1 (MQ=255)                      gTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGGGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGGCTAATTTGGAACCTACGGCGGCAAACAGATAAGGGGGGCCCGGGc                                                                                                              >  2:873071/1‑140 (MQ=255)                                     cAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAAtct                                                                                               >  1:686230/1‑140 (MQ=255)                                           gATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGc                                                                                         >  1:250589/1‑140 (MQ=255)                                             tAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTTGGTGccc                                                                                       >  1:292313/1‑139 (MQ=255)                                              aaaGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTATGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTc                                                                                      <  1:899118/140‑1 (MQ=255)                                                     tCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGt                                                                               <  2:711395/140‑1 (MQ=255)                                                     tCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGt                                                                               >  2:735784/1‑140 (MQ=255)                                                     tCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGGGCCTCCACGCGt                                                                               >  1:115332/1‑140 (MQ=255)                                                       cATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAg                                                                             >  1:187761/1‑140 (MQ=255)                                                          cGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCt                                                                          >  1:360231/1‑140 (MQ=255)                                                           gCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTg                                                                         <  2:676637/140‑1 (MQ=255)                                                           gCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTg                                                                         >  2:727466/1‑140 (MQ=255)                                                            ccATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGc                                                                        >  2:891975/1‑140 (MQ=255)                                                               tACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCggg                                                                     <  1:861762/140‑1 (MQ=255)                                                                aCCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGc                                                                    >  2:722629/1‑140 (MQ=255)                                                                aCCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGc                                                                    <  2:205593/140‑1 (MQ=255)                                                                    tCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAg                                                                <  1:722629/140‑1 (MQ=255)                                                                    tCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAg                                                                >  2:83418/1‑140 (MQ=255)                                                                     cgcgAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGc                                                               <  2:187761/140‑1 (MQ=255)                                                                            ggtgAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCa                                                        >  1:728898/1‑140 (MQ=255)                                                                            ggtgAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCa                                                        <  2:250589/140‑1 (MQ=255)                                                                                 aaaTTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATTCCAGCGGt                                                   <  2:115332/140‑1 (MQ=255)                                                                                 aaaTTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGt                                                   <  1:200293/140‑1 (MQ=255)                                                                                       gtgCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCtt                                             >  1:274327/1‑140 (MQ=255)                                                                                           gtcgtGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAAt                                         <  2:360231/140‑1 (MQ=255)                                                                                               tGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGTGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATg                                     <  1:490434/140‑1 (MQ=255)                                                                                               tGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATg                                     <  1:83418/140‑1 (MQ=255)                                                                                               tGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATg                                     <  2:591552/140‑1 (MQ=255)                                                                                                               ggcggTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCgg                     <  1:891975/140‑1 (MQ=255)                                                                                                                gcggTCTAATTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCggg                    <  2:292313/140‑1 (MQ=255)                                                                                                                   gTCTAATTCGGCACAGACAGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCAt                 >  2:267529/1‑140 (MQ=255)                                                                                                                        aTTCGGCACAGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATgccag            >  1:277258/1‑140 (MQ=255)                                                                                                                              cacaGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATgc               >  2:486523/1‑131 (MQ=255)                                                                                                                              cacaGACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATgc               <  1:486523/131‑1 (MQ=255)                                                                                                                                  gACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGAt  <  2:686230/140‑1 (MQ=255)                                                                                                                                  gACGGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGAt  <  1:197405/140‑1 (MQ=255)                                                                                                                                  gACAGCAGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGAt  <  1:267529/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGAT  >  NC_000913/186350‑186618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑