Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,197,918	T→C	intergenic (+620/‑2361)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,197,918	0	T	C	100.0%	192.2 / NA	54	intergenic (+620/‑2361)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/22);  total (32/22)

ATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGTGCTGGA  >  NC_000913/2197783‑2198050                                                                                                                                        |                                                                                                                                     aTGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGcc                                                                                                                                  <  2:846498/140‑1 (MQ=255)    cagcaATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTa                                                                                                                               <  1:116987/140‑1 (MQ=255)      gcaATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACt                                                                                                                             <  1:379105/140‑1 (MQ=255)           ggcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAAt                                                                                                                        >  2:170972/1‑140 (MQ=255)           ggcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAAt                                                                                                                        <  2:866413/140‑1 (MQ=255)                 aCTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGcc                                                                                                                  <  1:8686/140‑1 (MQ=255)                 aCTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGcc                                                                                                                  >  2:783738/1‑140 (MQ=255)                    gACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGc                                                                                                               <  1:170972/140‑1 (MQ=255)                    gACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGc                                                                                                               >  1:185223/1‑140 (MQ=255)                     aCGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGca                                                                                                              <  2:99246/140‑1 (MQ=255)                      cGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTTATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGcaa                                                                                                             >  1:139802/1‑140 (MQ=255)                          aCTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCACAGCTGCCGTACTGAAATCATGCCAGCaacaac                                                                                                         >  1:660126/1‑140 (MQ=255)                              cttGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTTATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGac                                                                                                     <  2:139802/140‑1 (MQ=255)                                   gCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGc                                                                                                >  1:48858/1‑140 (MQ=255)                                        aagaagAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCa                                                                                           >  2:667328/1‑140 (MQ=255)                                            agagAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAAcc                                                                                       >  1:79109/1‑140 (MQ=255)                                                          gcATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGAt                                                                         >  2:879743/1‑140 (MQ=255)                                                               aTGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACt                                                                    <  1:783738/140‑1 (MQ=255)                                                                  cTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGcc                                                                 <  1:853564/140‑1 (MQ=255)                                                                  cTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGcc                                                                 <  2:48858/140‑1 (MQ=255)                                                                          aaTGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCgtag                                                         >  2:92701/1‑140 (MQ=255)                                                                            tGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCgtagta                                                       >  1:232683/1‑140 (MQ=255)                                                                            tGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCgtagta                                                       <  2:735868/140‑1 (MQ=255)                                                                                  tctcACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGTCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTctcc                                                 >  1:181874/1‑140 (MQ=255)                                                                                  tctcACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAg                                                               >  2:514194/1‑126 (MQ=255)                                                                                  tctcACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAg                                                               <  1:514194/126‑1 (MQ=255)                                                                                       caACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTctcccctcc                                            >  2:280232/1‑140 (MQ=255)                                                                                       caACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTctcccctcc                                            <  1:280232/140‑1 (MQ=255)                                                                                        aaCCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTctcccctccc                                           >  1:597883/1‑140 (MQ=255)                                                                                        aaCCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTctcccctccc                                           >  1:338387/1‑140 (MQ=255)                                                                                        aaCCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTctcccctccc                                           >  2:345219/1‑140 (MQ=255)                                                                                             gCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCt                                      >  2:291063/1‑140 (MQ=255)                                                                                              cACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCtt                                     >  2:614902/1‑140 (MQ=255)                                                                                               aCTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCttt                                    >  1:118546/1‑140 (MQ=255)                                                                                                  ggCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCga                                 >  2:180782/1‑140 (MQ=255)                                                                                                       aaCAGGTCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCgaaaaag                            >  2:892166/1‑140 (MQ=255)                                                                                                             tCATTCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGaaaaaa                      >  2:483215/1‑140 (MQ=255)                                                                                                                 tCCCTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGc                  >  1:162234/1‑140 (MQ=255)                                                                                                                   ccTGGCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCg                <  2:162234/140‑1 (MQ=255)                                                                                                                      ggCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGAtt             >  2:179638/1‑140 (MQ=255)                                                                                                                      ggCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGAtt             >  1:87513/1‑140 (MQ=255)                                                                                                                      ggCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGAtt             >  1:48772/1‑140 (MQ=255)                                                                                                                      ggCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGAtt             >  1:173232/1‑140 (MQ=255)                                                                                                                      ggCTCTCGACACCCACAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGAtt             <  2:660126/140‑1 (MQ=255)                                                                                                                       gCTCTCGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAGAAAATCGCCGATTc            <  1:291063/140‑1 (MQ=255)                                                                                                                           tcGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGt        >  1:309523/1‑140 (MQ=255)                                                                                                                            cGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTc            <  2:361425/135‑1 (MQ=255)                                                                                                                            cGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTc            >  1:361425/1‑135 (MQ=255)                                                                                                                            cGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGTg       <  2:338387/140‑1 (MQ=255)                                                                                                                            cGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGTg       <  1:345219/140‑1 (MQ=255)                                                                                                                            cGACACCCGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGTg       >  1:185262/1‑140 (MQ=255)                                                                                                                                 cccGCAGCTGCCGTACTGAAATCATGTCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGTGCTGGa  <  2:181874/140‑1 (MQ=255)                                                                                                                                  ccGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCg          <  1:318157/131‑1 (MQ=255)                                                                                                                                  ccGCAGCTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCg          >  2:318157/1‑131 (MQ=255)                                                                                                                                        |                                                                                                                                     ATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCGCCGATCTACTGCCAGCCGTAGTAGTCTCCCCTCCCTGGCTTTCGAAAAAGAAAAAATCGCCGATTCCGGTGCTGGA  >  NC_000913/2197783‑2198050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑