Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,206,031	G→A	D113N (GAT→AAT)	yehM →	putative YehN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,206,031	0	G	A	100.0%	146.9 / NA	42	D113N (GAT→AAT)	yehM	putative YehN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/28);  total (14/28)

GTGGTGCCTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACGATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTACCCTCTGCGCTGGCGCA  >  NC_000913/2205906‑2206164                                                                                                                              |                                                                                                                                      gtggtgCCTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACg                                                                                                                         <  1:431829/140‑1 (MQ=255)  tggtgCCTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGc                                                                                                                        >  1:538851/1‑140 (MQ=255)  tggtgCCTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGc                                                                                                                        >  2:231536/1‑140 (MQ=255)   ggtgCCTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCa                                                                                                                       <  2:684878/140‑1 (MQ=255)        cTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATg                                                                                                                  <  2:627567/140‑1 (MQ=255)        cTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATg                                                                                                                  <  2:808896/140‑1 (MQ=255)            aCGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAg                                                                                                              <  2:613472/140‑1 (MQ=255)            aCGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAg                                                                                                              <  1:602321/140‑1 (MQ=255)             cGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGc                                                                                                             >  2:72590/1‑140 (MQ=255)               ccATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCa                                                                                                           >  2:785832/1‑140 (MQ=255)               ccATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCa                                                                                                           <  2:228408/140‑1 (MQ=255)                 aTTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAgg                                                                                                         >  2:352337/1‑140 (MQ=255)                  tttGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGc                                                                                                        <  2:105856/140‑1 (MQ=255)                      cTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTa                                                                                                    <  1:72590/140‑1 (MQ=255)                          aTTTTCGCCGGAGTGGCAGGCACTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTActgc                                                                                                >  2:837826/1‑140 (MQ=255)                              tCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCg                                                                                            >  1:912651/1‑140 (MQ=255)                                 ccGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGc                                                                                         <  2:194391/140‑1 (MQ=255)                                 ccGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGc                                                                                         <  1:723729/140‑1 (MQ=255)                                   ggAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCa                                                                                       >  1:188978/1‑140 (MQ=255)                                        ggCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGc                                                                                  <  2:188978/140‑1 (MQ=255)                                        ggCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGc                                                                                  <  1:290421/140‑1 (MQ=255)                                           aGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGACGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATg                                                                               <  1:22911/140‑1 (MQ=255)                                            ggCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATgg                                                                              >  1:793236/1‑140 (MQ=255)                                                cTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATaa                                                                          <  2:421213/140‑1 (MQ=255)                                                     aGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCg                                                                     <  2:46504/140‑1 (MQ=255)                                                       ccGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGAt                                                                   <  1:879498/140‑1 (MQ=255)                                                        cGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATaa                                                                          >  2:620078/1‑132 (MQ=255)                                                        cGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATaa                                                                          <  1:620078/132‑1 (MQ=255)                                                           aCGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATAccc                                                               <  1:837826/140‑1 (MQ=255)                                                           aCGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATAccc                                                               <  2:19713/140‑1 (MQ=255)                                                            cGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCt                                                              <  2:690624/140‑1 (MQ=255)                                                              tCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCtgt                                                            <  1:785832/140‑1 (MQ=255)                                                                 cATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTggg                                                         >  2:726614/1‑140 (MQ=255)                                                                              acttacttCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGa                                            <  2:912651/140‑1 (MQ=255)                                                                                     tCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGaa                                     <  2:538851/140‑1 (MQ=255)                                                                                        tCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGc                                  >  1:132497/1‑140 (MQ=255)                                                                                             ttGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCa                             <  1:231536/140‑1 (MQ=255)                                                                                                         gcgcAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTAcc                 <  1:726614/140‑1 (MQ=255)                                                                                                          cgcAGAGTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTAccc                >  1:190216/1‑140 (MQ=255)                                                                                                                gTGAAGAAGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTACCCTCTgcg          <  2:132497/140‑1 (MQ=255)                                                                                                                  gaagaaGAGGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTACCCTCTGCGCt        >  1:11005/1‑140 (MQ=255)                                                                                                                        gagGACAATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTACCCTCTGCGCTGGCGCa  <  2:190216/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                      GTGGTGCCTGGACGCCATTTGCTGAATTTTCGCCGGAGTGGCAGGCGCTACAAGCCGCACGTCGCATTCAGGCACAAACTTACTTCATCGATTTGCCTTGCTGGGCGCAGAGTGAAGAAGAGGACGATTCGCCTGATACGCAAGATGAAAGCCAGGCCTTACTGCTGCGTGCCACCCGCATGGATAACAGCGATACCCTGTGGGATCACTTGTTCGAAGATGAAAGCCAGCAAACTGCATTACCCTCTGCGCTGGCGCA  >  NC_000913/2205906‑2206164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑