Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,325,984	T→C	I410T (ATC→ACC)	atoE →	short chain fatty acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,325,984	0	T	C	100.0%	131.6 / NA	38	I410T (ATC→ACC)	atoE	short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

GTTATTCAGGGACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAA  >  NC_000913/2325848‑2326108                                                                                                                                         |                                                                                                                             gTTATTCAGGGACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGc                                                                                                                           <  1:235736/140‑1 (MQ=255)    aTTCAGGGACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCgg                                                                                                                        >  1:15151/1‑140 (MQ=255)          ggACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCgg                                                                                                                  >  1:400074/1‑140 (MQ=255)               tttCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGTCTCGGTGTcc                                                                                                             >  1:847149/1‑140 (MQ=255)                 tCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCcgc                                                                                                           >  2:82422/1‑140 (MQ=255)                 tCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCACTGCCAGCACTGGCAACCGCCGGACTCGGTGTCcgc                                                                                                           >  1:605346/1‑140 (MQ=255)                   gTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGa                                                                                                         <  2:407319/140‑1 (MQ=255)                        aCCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGAcatca                                                                                                    >  1:887338/1‑140 (MQ=255)                           cGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATgg                                                                                                 >  1:10939/1‑140 (MQ=255)                            gCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATggg                                                                                                >  1:650169/1‑140 (MQ=255)                             cAGCCCCGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACACCCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGc                                                                                               <  2:25466/140‑1 (MQ=255)                             cAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGc                                                                                               <  2:650169/140‑1 (MQ=255)                             cAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGc                                                                                               <  2:400074/140‑1 (MQ=255)                                  cAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTg                                                                                          <  2:10939/140‑1 (MQ=255)                                    ggcgctgggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCa                                                                                        >  2:487923/1‑140 (MQ=255)                                     gcgctgggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCAt                                                                                       >  2:626318/1‑140 (MQ=255)                                        ctgggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCGTCAc                                                                                    <  1:65786/140‑1 (MQ=255)                                          gggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTg                                                                                  >  1:753570/1‑140 (MQ=255)                                          gggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTg                                                                                  >  1:776596/1‑140 (MQ=255)                                           ggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGc                                                                                 <  1:487923/140‑1 (MQ=255)                                                                                  ggCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAAc                                          <  2:776596/140‑1 (MQ=255)                                                                                  ggCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTCAAc                                          <  1:626318/140‑1 (MQ=255)                                                                                  ggCGAGCAATGGATGAACAGGTCCCAGCGATTCGGGGCGCTGCGGGCGCTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAAc                                          <  2:298561/140‑1 (MQ=255)                                                                                   gCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACg                                         <  1:82422/140‑1 (MQ=255)                                                                                      aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCAt                                                   >  1:818529/1‑127 (MQ=255)                                                                                      aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCAt                                                   <  2:818529/127‑1 (MQ=255)                                                                                       gCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATCGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGt                                     <  2:819079/140‑1 (MQ=255)                                                                                       gCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGAGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGt                                     >  1:298561/1‑140 (MQ=255)                                                                                                  aCATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAc                          <  2:353050/140‑1 (MQ=255)                                                                                                   cATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGTCTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAcc                         <  2:847149/140‑1 (MQ=255)                                                                                                   cATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAcc                         >  2:285438/1‑140 (MQ=255)                                                                                                        cacaACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCATCCCTAc                    >  2:881277/1‑140 (MQ=255)                                                                                                        cacaACCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTAc                    <  2:15151/140‑1 (MQ=255)                                                                                                           aaCCATTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACaaa                 >  1:585620/1‑140 (MQ=255)                                                                                                               aTTCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGa             <  2:887338/140‑1 (MQ=255)                                                                                                                ttCTGGGCGCTGTCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGaa            <  1:193878/140‑1 (MQ=255)                                                                                                                 tCTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAg           >  2:741494/1‑140 (MQ=255)                                                                                                                  cTGGGCGCTGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCT‑‑TCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGaa        >  1:323516/1‑140 (MQ=255)                                                                                                                          tGCCAGCACTGGCAACCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATaaa  >  2:715095/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             GTTATTCAGGGACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAA  >  NC_000913/2325848‑2326108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑