Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,477,624 T→C E2E (GAA→GAG)  dsdC ← DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,6240TC100.0% 164.9 / NA 46E2E (GAA→GAG) dsdCDNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

TGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATT  >  NC_000913/2477487‑2477755
                                                                                                                                         |                                                                                                                                   
tGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTc                                                                                                                                   >  1:382446/1‑140 (MQ=255)
      aCCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAAt                                                                                                                             <  2:382446/140‑1 (MQ=255)
      aCCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAAt                                                                                                                             >  1:741786/1‑140 (MQ=255)
      aCCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTCATTTCACGAAGGGGCTCCATAAt                                                                                                                             <  2:395276/140‑1 (MQ=255)
            cTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTc                                                                                                                       >  1:503573/1‑140 (MQ=255)
              ggggCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCAt                                                                                                                     >  2:587147/1‑140 (MQ=255)
                ggCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCAttt                                                                                                                   >  2:77012/1‑140 (MQ=255)
                     aGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGc                                                                                                              >  2:853257/1‑140 (MQ=255)
                     aGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGc                                                                                                              <  2:503573/140‑1 (MQ=255)
                     aGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGc                                                                                                              >  1:388112/1‑140 (MQ=255)
                      gCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCt                                                                                                             <  2:765646/140‑1 (MQ=255)
                         aCAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTaa                                                                                                          >  1:247824/1‑140 (MQ=255)
                         aCAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTaa                                                                                                          >  2:283102/1‑140 (MQ=255)
                                 tctGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTaaaa                                                                                                  >  2:776913/1‑140 (MQ=255)
                                        ccAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATACATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGc                                                                                           >  2:609155/1‑140 (MQ=255)
                                           gggCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGc                                                                                        <  1:227044/140‑1 (MQ=255)
                                                         tGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTat                                                                          >  2:606082/1‑140 (MQ=255)
                                                            cTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgag                                                                       <  1:762988/140‑1 (MQ=255)
                                                             tGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgaga                                                                      >  1:301042/1‑140 (MQ=255)
                                                              ggCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGAGGCAAAATTTCAGCTatgagat                                                                     >  1:647551/1‑140 (MQ=255)
                                                                    cACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTa                                                               <  2:242828/140‑1 (MQ=255)
                                                                         cAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCt                                                                            <  1:170201/122‑1 (MQ=255)
                                                                         cAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCt                                                                            >  2:170201/1‑122 (MQ=255)
                                                                         cAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTg                                                          <  2:741786/140‑1 (MQ=255)
                                                                           aaaGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGaa                                                        <  1:587147/140‑1 (MQ=255)
                                                                                 tGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgagatgag                                                                  >  1:862362/1‑124 (MQ=255)
                                                                                 tGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgagatgag                                                                  <  2:862362/124‑1 (MQ=255)
                                                                                 tGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCatat                                                  >  2:688510/1‑140 (MQ=255)
                                                                                       tttGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAAt                                            <  1:606082/140‑1 (MQ=255)
                                                                                         tGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTc                                          >  2:487729/1‑140 (MQ=255)
                                                                                                tGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCa                                   >  2:916979/1‑140 (MQ=255)
                                                                                                tGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCa                                   >  2:654873/1‑140 (MQ=255)
                                                                                                tGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCa                                   >  2:418089/1‑140 (MQ=255)
                                                                                                    aGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCAtttt                               <  1:283102/140‑1 (MQ=255)
                                                                                                     gCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCAttttt                              <  2:301042/140‑1 (MQ=255)
                                                                                                         ttAAGCAGCCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAAATTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGAt                          <  2:903332/140‑1 (MQ=255)
                                                                                                              cagcCGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTaaa                     <  2:647551/140‑1 (MQ=255)
                                                                                                                 ccGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCa                  >  2:576080/1‑140 (MQ=255)
                                                                                                                 ccGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCa                  >  1:120707/1‑140 (MQ=255)
                                                                                                                  cGATTTCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCAc                 <  1:776913/140‑1 (MQ=255)
                                                                                                                      ttCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAAt                    >  1:315909/1‑133 (MQ=255)
                                                                                                                      ttCTTATTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAAt                    <  2:315909/133‑1 (MQ=255)
                                                                                                                           aTTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGAt        <  1:198336/140‑1 (MQ=255)
                                                                                                                           aTTTCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGAt        <  1:916979/140‑1 (MQ=255)
                                                                                                                            tttCACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATg       <  1:635372/140‑1 (MQ=255)
                                                                                                                               cACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTGAATTCACCTTCATTTTTAGATGTAAATCATTCCATTGATGCaa    <  1:853257/140‑1 (MQ=255)
                                                                                                                               cACGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa    >  2:388151/1‑140 (MQ=255)
                                                                                                                                 cGAAGGGGCTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAtt  >  1:171614/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                   
TGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATT  >  NC_000913/2477487‑2477755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑