Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,480,487	T→C	V429A (GTG→GCG)	dsdA →	D‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,480,487	0	T	C	100.0%	130.1 / NA	37	V429A (GTG→GCG)	dsdA	D‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/15);  total (22/15)

GCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCC  >  NC_000913/2480366‑2480614                                                                                                                          |                                                                                                                                gCGGGGATGGGCGGGCCTCAGCGCGGGTGGGCATCAGGAAGTTTTCAACAGAAGCACGGGTTCAGCGCAGAACAACTGCGTAATAACACCCCTCTGGTGGGGGGGACGGGGGGGGGGATGGGGCCGGGAGAAAAGATGaa                                                                                                               >  2:173442/1‑140 (MQ=255)              gACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCa                                                                                                  >  1:622528/1‑140 (MQ=255)                     gcgcGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGcc                                                                                           <  1:751351/140‑1 (MQ=255)                             gtgCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTtaataa                                                                                   <  2:535535/140‑1 (MQ=255)                                  tCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGttt                                                                              >  1:428015/1‑140 (MQ=255)                                    aGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCa                                                                            >  1:304253/1‑140 (MQ=255)                                          ttACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACgcag                                                                      <  1:173442/140‑1 (MQ=255)                                          ttACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACgcag                                                                      <  1:58227/140‑1 (MQ=255)                                           tACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACgcagc                                                                     >  2:312448/1‑140 (MQ=255)                                              cAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATc                                                                  >  1:314100/1‑140 (MQ=255)                                                  aGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa                                                              >  1:446886/1‑140 (MQ=255)                                                    aTGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATc                                                            >  1:270497/1‑140 (MQ=255)                                                         cGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATc                                                            <  2:753931/135‑1 (MQ=255)                                                         cGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTCGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATc                                                            >  1:753931/1‑135 (MQ=255)                                                                    cAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt                                                          >  2:65237/1‑126 (MQ=255)                                                                    cAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt                                                          <  1:65237/126‑1 (MQ=255)                                                                    cAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGc                                            >  1:197974/1‑140 (MQ=255)                                                                                 tAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATg                               >  1:688129/1‑140 (MQ=255)                                                                                 tAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATg                               >  1:252635/1‑140 (MQ=255)                                                                                 tAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAGGCTGCCGATg                               >  2:795105/1‑140 (MQ=255)                                                                                    tACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGgcg                            <  2:446886/140‑1 (MQ=255)                                                                                    tACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGgcg                            <  1:312448/140‑1 (MQ=255)                                                                                        aCTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGa                        <  1:215779/140‑1 (MQ=255)                                                                                         cTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGAc                       >  2:879624/1‑140 (MQ=255)                                                                                         cTCATCTGGTGTGGGCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGAc                       >  1:694194/1‑140 (MQ=255)                                                                                                     gggCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCg           >  2:545312/1‑140 (MQ=255)                                                                                                      ggCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCgg          >  2:23440/1‑140 (MQ=255)                                                                                                      ggCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCgg          >  1:418366/1‑140 (MQ=255)                                                                                                      ggCGACGGGAGGTGGAATGGCGACGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCgg          >  2:66145/1‑140 (MQ=255)                                                                                                       gCGACGGGAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGt         >  2:875523/1‑140 (MQ=255)                                                                                                             ggAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAcc   <  2:314100/140‑1 (MQ=255)                                                                                                             ggAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAcc   <  2:428015/140‑1 (MQ=255)                                                                                                             ggAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAcc   <  2:197974/140‑1 (MQ=255)                                                                                                             ggAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAcc   <  1:875523/140‑1 (MQ=255)                                                                                                              gAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAccc  <  2:304253/140‑1 (MQ=255)                                                                                                              gAGGTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAccc  >  1:22629/1‑140 (MQ=255)                                                                                                                 gTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAcc   >  2:572523/1‑136 (MQ=255)                                                                                                                 gTGGAATGGCGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTAcc   <  1:572523/136‑1 (MQ=255)                                                                                                                          |                                                                                                                                GCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCC  >  NC_000913/2480366‑2480614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑