Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,445	T→C	intergenic (+118/+23)	ypeC → / ← mntH	DUF2502 domain‑containing protein YpeC/Mn(2(+))/Fe(2(+)): H(+) symporter MntH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,445	0	T	C	100.0%	156.9 / NA	44	intergenic (+118/+23)	ypeC/mntH	DUF2502 domain‑containing protein YpeC/Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/18);  total (26/18)

CGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAA  >  NC_000913/2511319‑2511581                                                                                                                               |                                                                                                                                         cccctttAACCGCGCCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTGGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACg                                                                                                                             <  2:491962/135‑1 (MQ=37) cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACg                                                                                                                             >  1:435986/1‑140 (MQ=255)    cATTAACCGGGCCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTTTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTc                                                                                                                          <  2:525178/140‑1 (MQ=37)       tAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCAtt                                                                                                                       >  2:385014/1‑140 (MQ=255)              gTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTAc                                                                                                                >  1:256991/1‑140 (MQ=255)              gTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTAc                                                                                                                >  2:785850/1‑140 (MQ=255)                   ccAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTTTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATcc                                                                                                           <  2:229893/140‑1 (MQ=255)                       tGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAgc                                                                                                       >  2:700578/1‑140 (MQ=255)                           gCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCg                                                                                                   <  1:785850/140‑1 (MQ=255)                            cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGt                                                                                                  >  2:840687/1‑140 (MQ=255)                            cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGt                                                                                                  >  2:319334/1‑140 (MQ=255)                              caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTcc                                                                                                >  1:728964/1‑140 (MQ=255)                               aaTGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTccc                                                                                               <  2:572753/140‑1 (MQ=255)                                 tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATcc                                                                                                           <  2:792985/126‑1 (MQ=255)                                 tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATcc                                                                                                           >  1:792985/1‑126 (MQ=255)                                 tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                             >  2:600718/1‑140 (MQ=255)                                 tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                             >  2:623000/1‑140 (MQ=255)                                 tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                             >  1:778151/1‑140 (MQ=255)                                     tGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaa                                                                                         <  1:385014/140‑1 (MQ=255)                                      gATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaac                                                                                        >  1:180152/1‑140 (MQ=255)                                      gATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaac                                                                                        >  1:601669/1‑140 (MQ=255)                                       aTGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaaca                                                                                       <  2:778151/140‑1 (MQ=255)                                       aTGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaaca                                                                                       <  2:534004/140‑1 (MQ=255)                                         gCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCaacaac                                                                                     >  2:19527/1‑140 (MQ=255)                                              gCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGat                                                                                >  1:377246/1‑140 (MQ=255)                                                       tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAgcgc                                                                       >  1:492041/1‑140 (MQ=255)                                                        cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGcc                                                                      >  1:673497/1‑140 (MQ=255)                                                            tCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGa                                                                  >  1:258727/1‑140 (MQ=255)                                                                 ccTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                             >  2:445964/1‑140 (MQ=255)                                                                     cAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcacca                                                         >  2:458947/1‑140 (MQ=255)                                                                     cAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcacca                                                         <  2:601669/140‑1 (MQ=255)                                                                                       cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtct                                       >  2:387146/1‑140 (MQ=255)                                                                                            ggCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGtttt                                  >  2:364235/1‑140 (MQ=255)                                                                                            ggCGGATAAGGCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGtttt                                  >  2:158816/1‑140 (MQ=255)                                                                                                     ggCGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTg                         <  1:623000/140‑1 (MQ=255)                                                                                                       tgTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAAGCACCCAGCCTGTCTGTTTTACGCGTTTGCt                       <  1:700578/139‑1 (MQ=255)                                                                                                       cGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                       <  2:180152/140‑1 (MQ=255)                                                                                                       cGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                       <  2:735553/140‑1 (MQ=255)                                                                                                       cGTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                       <  1:600718/140‑1 (MQ=255)                                                                                                        gTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTg                      <  1:319334/140‑1 (MQ=255)                                                                                                        gTTTACGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTg                      <  1:840687/140‑1 (MQ=255)                                                                                                            aCGCCGCATCCGCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCa                  >  2:252915/1‑140 (MQ=255)                                                                                                                       gCCAATAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGccc       >  1:576981/1‑140 (MQ=255)                                                                                                                            tAGCGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCaa  <  1:158816/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         CGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAA  >  NC_000913/2511319‑2511581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑