Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,526,738	T→C	E40G (GAA→GGA)	yfeR ←	putative LysR‑type DNA‑binding transcriptional regulator YfeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,526,738	0	T	C	100.0%	132.9 / NA	37	E40G (GAA→GGA)	yfeR	putative LysR‑type DNA‑binding transcriptional regulator YfeR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

CCGGTATCGCGCAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTTCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTTTCTTCATG  >  NC_000913/2526603‑2526870                                                                                                                                             |                                                                                                                                     ccGGTATCGCGCAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCtt                                                                                                                                  >  2:297915/1‑140 (MQ=255)   ggTATCGCGCAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTca                                                                                                                                <  2:362745/140‑1 (MQ=255)     tATCGCGCAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTcaca                                                                                                                              <  1:613771/140‑1 (MQ=255)     tATCGCGCAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTcaca                                                                                                                              <  2:136921/140‑1 (MQ=255)     tATCGCGCAACGTGCTGTTCAGTTCAACCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTcaca                                                                                                                              <  1:306096/140‑1 (MQ=255)         gcgcAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACtgt                                                                                                                          >  2:179749/1‑140 (MQ=255)          cgcAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACtgtg                                                                                                                         >  2:764068/1‑140 (MQ=255)                                ccAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAgg                                                                                                   >  2:799413/1‑140 (MQ=255)                                     agTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGAt                                                                                              >  2:242229/1‑140 (MQ=255)                                        cGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACg                                                                                           <  1:258361/140‑1 (MQ=255)                                         gCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGc                                                                                          <  1:764068/140‑1 (MQ=255)                                           tCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGctc                                                                                        <  1:648661/140‑1 (MQ=255)                                                            ctgctgCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAAc                                                                       >  1:508030/1‑140 (MQ=255)                                                                tgcCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACtttt                                                                   >  2:187911/1‑140 (MQ=255)                                                                      gCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTg                                                             <  1:179749/140‑1 (MQ=255)                                                                        aTCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTgcg                                                           <  1:904442/140‑1 (MQ=255)                                                                           tGTAAGCACCACTTCACGCGTGGTTCTGTCCTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTg                                                             <  2:473880/140‑1 (MQ=255)                                                                            gTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTgcg                                                           <  1:353109/136‑1 (MQ=255)                                                                            gTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTgcg                                                           >  2:353109/1‑136 (MQ=255)                                                                            gTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTAc                                                       >  2:284929/1‑140 (MQ=255)                                                                                gCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACtt                                                                     >  2:679286/1‑122 (MQ=255)                                                                                gCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACtt                                                                     <  1:679286/122‑1 (MQ=255)                                                                                   cgccTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACg                                                <  1:445771/137‑1 (MQ=255)                                                                                    cacTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTACACGACTAAAACTTTTCTCCTGCGCTACTGTGACGa                                               >  1:56203/1‑140 (MQ=255)                                                                                      cTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGaaa                                             <  1:297915/140‑1 (MQ=255)                                                                                       ttCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGaaaa                                            >  1:195761/1‑140 (MQ=255)                                                                                              gTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTaa                                     >  2:37540/1‑140 (MQ=255)                                                                                               tGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAAt                                    <  2:681607/140‑1 (MQ=255)                                                                                                                gcagGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGaa                        <  1:799413/140‑1 (MQ=255)                                                                                                                 cagGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGaat                       >  1:494092/1‑140 (MQ=255)                                                                                                                 cagGCGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGaat                       >  1:274696/1‑140 (MQ=255)                                                                                                                     cGAACACCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAAtt                   <  2:274696/140‑1 (MQ=255)                                                                                                                        acacCAGTATGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCAt                <  2:93199/140‑1 (MQ=255)                                                                                                                                aTGATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTttc        <  2:402214/140‑1 (MQ=255)                                                                                                                                  gATTTTCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTttctt      <  1:688677/140‑1 (MQ=255)                                                                                                                                    ttttCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTTTCTTCa    <  2:195761/140‑1 (MQ=255)                                                                                                                                      ttCCAGTCCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTTTCTTCATg  >  2:677284/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                     CCGGTATCGCGCAACGTGCTGTTCAGTTCATCCAGCAGTCGCTCAAGACGCAAAGCCAGCTGCTGCCCTGCATCTGTAAGCACCACTTCACGCGTGGTT‑‑‑‑‑CTGTCCAGCAGGCGAACACCAGTATGATTTTCCAGTTCCTTCACACTGTGACTCACTGCCGACTGGCTCAGGCCGATACGCTCTCCTGCACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTTTCTTCATG  >  NC_000913/2526603‑2526870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑