Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,586,334	A→G	T202A (ACC→GCC)	narQ →	sensory histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,586,334	0	A	G	100.0%	205.3 / NA	57	T202A (ACC→GCC)	narQ	sensory histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/24);  total (33/24)

CGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCG  >  NC_000913/2586201‑2586467                                                                                                                                      |                                                                                                                                      cggcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAAcc                                                                                                                                 >  1:821455/1‑140 (MQ=255)   gcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGTTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTg                                                                                                                               <  2:821455/140‑1 (MQ=255)   gcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTg                                                                                                                               <  2:241371/140‑1 (MQ=255)        ggTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGaa                                                                                                                          <  2:93903/140‑1 (MQ=255)              ttCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCt                                                                                                                    <  1:97912/140‑1 (MQ=255)                 aCGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTgg                                                                                                                 >  2:300708/1‑140 (MQ=255)                 aCGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTgg                                                                                                                 >  2:31784/1‑140 (MQ=255)                      ggTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTCGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGc                                                                                                            >  2:88493/1‑140 (MQ=255)                       gTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCt                                                                                                           >  2:116352/1‑140 (MQ=255)                       gTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCt                                                                                                           >  2:492446/1‑140 (MQ=255)                       gTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGACGCCGCTCGATGCCAACCTGCCGAATGAGCTTGGTCTGCt                                                                                                           >  2:810660/1‑140 (MQ=255)                           tttttACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCa                                                                                                       >  2:128885/1‑140 (MQ=255)                             tttACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCaaa                                                                                                     <  2:503273/140‑1 (MQ=255)                             tttACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCaaa                                                                                                     <  2:369264/140‑1 (MQ=255)                                   ttGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCtt                                                                                               >  1:78266/1‑140 (MQ=255)                                      cgccgcATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCaaa                                                                                                     <  2:280085/131‑1 (MQ=255)                                      cgccgcATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCaaa                                                                                                     >  1:280085/1‑131 (MQ=255)                                      cgccgcATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTaa                                                                                            >  2:756328/1‑140 (MQ=255)                                      cgccgcATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTaa                                                                                            >  2:88059/1‑140 (MQ=255)                                      cgccgcATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTaa                                                                                            >  2:89889/1‑140 (MQ=255)                                       gccgcATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACGCGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAAc                                                                                           <  1:577037/140‑1 (MQ=255)                                          gcATACGCCATCAGGTGGTCGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAg                                                                                        <  1:88493/140‑1 (MQ=255)                                                     cAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTcgagcga                                                                             <  1:399555/140‑1 (MQ=255)                                                      aGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTcgagcgag                                                                            >  2:449616/1‑140 (MQ=255)                                                        gtggtTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCt                                                                          >  2:656868/1‑140 (MQ=255)                                                            ttGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCAt                                                                      >  2:433706/1‑140 (MQ=255)                                                            ttGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCAt                                                                      <  1:89889/140‑1 (MQ=255)                                                               ccccGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATaaa                                                                   <  1:300708/140‑1 (MQ=255)                                                               ccccGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATaaa                                                                   <  1:31784/140‑1 (MQ=255)                                                                cccGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAAt                                                                  >  2:29321/1‑140 (MQ=255)                                                                        aTCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGt                                                          <  1:339432/140‑1 (MQ=255)                                                                         tCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGtt                                                         <  1:492446/140‑1 (MQ=255)                                                                              tGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTg                                                    <  1:116352/140‑1 (MQ=255)                                                                               ggTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTgg                                                   >  1:702275/1‑140 (MQ=255)                                                                                 ttACCGCCAGTCAGCGTATTGAACACGGGCAGTTGGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGaa                                                 >  2:813624/1‑140 (MQ=255)                                                                                 ttACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGaa                                                 >  2:353217/1‑140 (MQ=255)                                                                                   aCCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGc                                               <  1:449616/140‑1 (MQ=255)                                                                                      gCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTc                                            >  2:467541/1‑140 (MQ=255)                                                                                       ccAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCa                                           >  2:866705/1‑140 (MQ=255)                                                                                          gTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTa                                        >  2:870381/1‑140 (MQ=255)                                                                                           tCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTag                                       <  1:810660/140‑1 (MQ=255)                                                                                                     gAACACGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc                             >  1:578634/1‑140 (MQ=255)                                                                                                         acGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgc                         >  1:571931/1‑140 (MQ=255)                                                                                                          cGGGCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg                        >  2:4463/1‑140 (MQ=255)                                                                                                           gggCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGa                       >  2:596283/1‑140 (MQ=255)                                                                                                           gggCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGa                       >  1:381131/1‑140 (MQ=255)                                                                                                            ggCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg                        <  2:730708/138‑1 (MQ=255)                                                                                                            ggCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg                        >  1:730708/1‑138 (MQ=255)                                                                                                            ggCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAt                      >  1:357572/1‑140 (MQ=255)                                                                                                            ggCAGTTCGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAt                      >  1:100407/1‑140 (MQ=255)                                                                                                                   cGACTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACg               <  1:433706/140‑1 (MQ=255)                                                                                                                      cTCGCCGCCGCTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAgg            >  1:561193/1‑140 (MQ=255)                                                                                                                           cgccgcTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg                        <  1:38873/123‑1 (MQ=255)                                                                                                                           cgccgcTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg                        >  2:38873/1‑123 (MQ=255)                                                                                                                             ccgcTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGcg     <  2:74871/140‑1 (MQ=255)                                                                                                                             ccgcTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGcg     <  2:578634/140‑1 (MQ=255)                                                                                                                                cTGGATGCCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGcgtcg  <  1:29321/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCG  >  NC_000913/2586201‑2586467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑