Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,608,676	T→C	F64L (TTT→CTT)	hyfG →	hydrogenase 4 catalytic subunit HyfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,608,676	0	T	C	100.0%	185.8 / NA	52	F64L (TTT→CTT)	hyfG	hydrogenase 4 catalytic subunit HyfG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

GTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGTCACA  >  NC_000913/2608546‑2608810                                                                                                                                   |                                                                                                                                       gTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAacg                                                                                                                               >  1:655219/1‑140 (MQ=255)   tCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAacgac                                                                                                                             >  2:702552/1‑140 (MQ=255)         gcgcGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCgg                                                                                                                       <  1:702552/140‑1 (MQ=255)         gcgcGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGCTGAAAATCATTCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCgg                                                                                                                       <  2:821797/140‑1 (MQ=255)          cgcgGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGa                                                                                                                      <  1:629778/140‑1 (MQ=255)            cgGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGacc                                                                                                                    >  2:205345/1‑139 (MQ=255)               tGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACtt                                                                                                                 >  2:909301/1‑140 (MQ=255)                         agagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCAtt                                                                                                       >  2:882897/1‑140 (MQ=255)                         agagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCAtt                                                                                                       >  2:568185/1‑140 (MQ=255)                           agCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTAc                                                                                                     <  1:205345/140‑1 (MQ=255)                             cGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTAcgc                                                                                                   <  1:5763/140‑1 (MQ=255)                              gACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTAcgcg                                                                                                  >  2:180777/1‑140 (MQ=255)                              gACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTAcgcg                                                                                                  >  2:244342/1‑140 (MQ=255)                              gACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTAcgcg                                                                                                  <  1:882897/140‑1 (MQ=255)                                cAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGt                                                                                                <  2:443755/140‑1 (MQ=255)                                  aaCGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGttt                                                                                              >  1:317651/1‑140 (MQ=255)                                          aaCAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGcc                                                                                      <  1:909301/140‑1 (MQ=255)                                            cAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCt                                                                                    <  2:505911/140‑1 (MQ=255)                                            cAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCt                                                                                    <  2:782847/140‑1 (MQ=255)                                            cAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCt                                                                                    <  2:655219/140‑1 (MQ=255)                                                tCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCa                                                                                <  1:568185/140‑1 (MQ=255)                                                         cGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAggg                                                                       >  2:297659/1‑140 (MQ=255)                                                         cGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAggg                                                                       >  1:531159/1‑140 (MQ=255)                                                              aaaaTCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAgggg                                                                      >  1:221750/1‑136 (MQ=255)                                                              aaaaTCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAgggg                                                                      <  2:221750/136‑1 (MQ=255)                                                              aaaaTCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGa                                                                  >  2:281989/1‑140 (MQ=255)                                                                    aatcTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATg                                                            <  1:180777/140‑1 (MQ=255)                                                                            gcCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATtg                                                    >  2:135287/1‑140 (MQ=255)                                                                            gcCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGACCGAAAAATGCTGGATtg                                                    <  2:98775/140‑1 (MQ=255)                                                                             ccTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaa                                                                 >  2:551419/1‑126 (MQ=255)                                                                             ccTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaa                                                                 <  1:551419/126‑1 (MQ=255)                                                                             ccTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGa                                                       <  2:557100/136‑1 (MQ=255)                                                                             ccTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGa                                                       >  1:557100/1‑136 (MQ=255)                                                                             ccTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATtgt                                                   >  1:721255/1‑140 (MQ=255)                                                                                 aCGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTgtggtg                                               <  1:244342/140‑1 (MQ=255)                                                                                    ttGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAg                                            >  2:364808/1‑140 (MQ=255)                                                                                       tACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGgcg                                         >  1:841774/1‑140 (MQ=255)                                                                                            tATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGt                                    >  2:327638/1‑140 (MQ=255)                                                                                            tATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGt                                    >  1:780764/1‑140 (MQ=255)                                                                                            tATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGt                                    >  1:518165/1‑140 (MQ=255)                                                                                              tCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCg                                  <  2:317651/140‑1 (MQ=255)                                                                                                   attatCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGt                                    <  2:545541/133‑1 (MQ=255)                                                                                                   attatCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGt                                    >  1:545541/1‑133 (MQ=255)                                                                                                      atCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGAc                          >  2:192508/1‑140 (MQ=255)                                                                                                      atCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGAc                          >  1:353995/1‑140 (MQ=255)                                                                                                      atCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGAc                          >  2:756267/1‑140 (MQ=255)                                                                                                      atCAACATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGAc                          >  2:777630/1‑140 (MQ=255)                                                                                                         aaCATGATGGCTGGCTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGt                       >  2:415613/1‑140 (MQ=255)                                                                                                                tggctggcTTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGt                <  2:802638/140‑1 (MQ=255)                                                                                                                 ggctggctTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGtt               >  1:488280/1‑140 (MQ=255)                                                                                                                     ggctTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCg           <  1:777630/140‑1 (MQ=255)                                                                                                                      gctTCCGGTCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCgt          >  1:89835/1‑140 (MQ=255)                                                                                                                              tCCTGCTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGTcaca  <  1:297659/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       GTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGTCACA  >  NC_000913/2608546‑2608810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑