Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,631,085	T→C	H484R (CAC→CGC)	guaA ←	GMP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,631,085	0	T	C	100.0%	236.5 / NA	65	H484R (CAC→CGC)	guaA	GMP synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/32);  total (33/32)

CAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTACAGGT  >  NC_000913/2630948‑2631220                                                                                                                                          |                                                                                                                                        cAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAgcgt                                                                                                                                       >  1:128542/1‑140 (MQ=255) cAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAgcgt                                                                                                                                       >  2:604208/1‑140 (MQ=255)    ggTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCg                                                                                                                                    <  1:599937/140‑1 (MQ=255)     gTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCgg                                                                                                                                   >  1:385094/1‑140 (MQ=255)         aaTCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCAt                                                                                                                               >  2:539547/1‑140 (MQ=255)              ttCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGt                                                                                                                          <  1:787262/140‑1 (MQ=255)                cccACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCg                                                                                                                        <  2:360756/140‑1 (MQ=255)                cccACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCg                                                                                                                        >  1:360756/1‑140 (MQ=255)                 ccACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGa                                                                                                                       <  2:919291/140‑1 (MQ=255)                         tGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCg                                                                                                               >  1:797174/1‑140 (MQ=255)                           gTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGAc                                                                                                             <  1:739609/140‑1 (MQ=255)                             aGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAg                                                                                                           <  2:644984/140‑1 (MQ=255)                              gCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGc                                                                                                          >  2:318632/1‑140 (MQ=255)                               cTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATTGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCa                                                                                                         <  1:849812/140‑1 (MQ=255)                               cTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCa                                                                                                         <  2:305468/140‑1 (MQ=255)                                   cggcTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCa                                                                                                     >  1:765593/1‑140 (MQ=255)                                   cggcTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCa                                                                                                     >  2:736080/1‑140 (MQ=255)                                         gCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAgagaga                                                                                               <  1:318632/140‑1 (MQ=255)                                            gCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCagag                                                                                                  <  1:573334/134‑1 (MQ=255)                                            gCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCagag                                                                                                  >  2:573334/1‑134 (MQ=255)                                            gCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACaa                                                                                            <  2:765593/140‑1 (MQ=255)                                                  gTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGt                                                                                      <  2:192792/140‑1 (MQ=255)                                                    cATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCa                                                                                    >  1:97805/1‑140 (MQ=255)                                                       acacCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGCCAACCCCGTCATAc                                                                                 >  2:33204/1‑140 (MQ=255)                                                          ccacGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTa                                                                              >  1:175958/1‑140 (MQ=255)                                                           cacGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTAc                                                                             >  1:297508/1‑140 (MQ=255)                                                             cgcgGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGa                                                                           >  2:791212/1‑140 (MQ=255)                                                             cgcgGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGa                                                                           <  2:297508/140‑1 (MQ=255)                                                             cgcgGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGa                                                                           >  2:279060/1‑140 (MQ=255)                                                             cgcgGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGa                                                                           >  1:121024/1‑140 (MQ=255)                                                             cgcgGGAAATACCGTCCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGa                                                                           >  2:609447/1‑140 (MQ=255)                                                               cgGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGAcc                                                                         >  2:276996/1‑140 (MQ=255)                                                                gggAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCa                                                                        <  1:830866/140‑1 (MQ=255)                                                                 ggAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCAt                                                                       >  2:412995/1‑140 (MQ=255)                                                                  gAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATc                                                                      <  1:791212/140‑1 (MQ=255)                                                                   aaaTACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCg                                                                     <  2:490792/140‑1 (MQ=255)                                                                   aaaTACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCg                                                                     <  1:604208/140‑1 (MQ=255)                                                                     aTACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGcc                                                                   >  2:110844/1‑140 (MQ=255)                                                                          gTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTa                                                              >  2:478781/1‑140 (MQ=255)                                                                          gTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTa                                                              >  1:330909/1‑140 (MQ=255)                                                                            tCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACg                                                            >  2:544923/1‑140 (MQ=255)                                                                             cACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGc                                                           <  1:539547/140‑1 (MQ=255)                                                                             cACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGc                                                           >  1:259435/1‑140 (MQ=255)                                                                                    ttGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGa                                                    <  2:702671/140‑1 (MQ=255)                                                                                     tGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGaa                                                   >  1:779902/1‑140 (MQ=255)                                                                                       aTAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACg                                                 <  1:110844/140‑1 (MQ=255)                                                                                          aTGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAc                                              >  2:123439/1‑140 (MQ=255)                                                                                          aTGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAc                                              >  1:766164/1‑140 (MQ=255)                                                                                          aTGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAc                                              >  1:357059/1‑140 (MQ=255)                                                                                           tGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAcc                                             <  1:412995/140‑1 (MQ=255)                                                                                                     aaCGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca                                   <  2:779902/140‑1 (MQ=255)                                                                                                       cgcgACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCCCCCATTACGCCAACGGAACGTACCGGCAGGAAcaca                                 >  2:79552/1‑140 (MQ=255)                                                                                                          gACCGAGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTg                              >  2:510523/1‑140 (MQ=255)                                                                                                               aGGAAATCGTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGc                         >  1:711772/1‑140 (MQ=255)                                                                                                                       gTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGa                 <  2:797174/140‑1 (MQ=255)                                                                                                                       gTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGa                 >  2:666024/1‑140 (MQ=255)                                                                                                                       gTACGGCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGa                 <  1:478781/140‑1 (MQ=255)                                                                                                                           ggCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACttt             <  2:121024/140‑1 (MQ=255)                                                                                                                            gCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTg            >  1:844619/1‑140 (MQ=255)                                                                                                                            gCAGATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTg            <  1:736080/140‑1 (MQ=255)                                                                                                                               gATGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCg         >  2:227700/1‑140 (MQ=255)                                                                                                                                aTGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGa                 >  1:854238/1‑131 (MQ=255)                                                                                                                                aTGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGa                 <  2:854238/131‑1 (MQ=255)                                                                                                                                 tGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTa       <  2:432381/140‑1 (MQ=255)                                                                                                                                 tGCGCCCAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTa       <  1:544923/140‑1 (MQ=255)                                                                                                                                      ccAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTACAGGt  <  1:276996/140‑1 (MQ=255)                                                                                                                                      ccAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTACAGGt  <  2:357059/140‑1 (MQ=255)                                                                                                                                      ccAGCGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCCCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTACAGGt  <  1:609447/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGACTTTGTCGTACAGGT  >  NC_000913/2630948‑2631220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑