Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,657,686	A→G	V417A (GTG→GCG)	hscA ←	iron‑sulfur cluster biosynthesis chaperone HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,657,686	0	A	G	100.0%	132.1 / NA	38	V417A (GTG→GCG)	hscA	iron‑sulfur cluster biosynthesis chaperone HscA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/14);  total (24/14)

TAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCG  >  NC_000913/2657550‑2657815                                                                                                                                         |                                                                                                                                  tAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGccgccg                                                                                                                                >  2:375530/1‑140 (MQ=255)  aGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCgg                                                                                                                               <  1:819955/140‑1 (MQ=255)  aGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCgg                                                                                                                               <  1:748802/140‑1 (MQ=255)          aaTACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTgg                                                                                                                       <  2:664680/140‑1 (MQ=255)                   cAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTAcgcg                                                                                                              <  2:354383/140‑1 (MQ=255)                    aGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCgg                                                                                                             >  2:154389/1‑140 (MQ=255)                      cgcAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGaa                                                                                                           >  1:391719/1‑140 (MQ=255)                         aaaaCGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCa                                                                                                        >  2:128182/1‑140 (MQ=255)                                     tGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTccacca                                                                                            >  1:756095/1‑140 (MQ=255)                                       aGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAgg                                                                                          >  1:661349/1‑140 (MQ=255)                                           gCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGgccgc                                                                                      <  2:894439/140‑1 (MQ=255)                                                 cTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCg                                                                                >  1:524679/1‑140 (MQ=255)                                                         gTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAgg                                                                        >  2:67605/1‑140 (MQ=255)                                                              cgcTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAg                                                                   <  2:391719/140‑1 (MQ=255)                                                              cgcTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAg                                                                   >  1:472617/1‑140 (MQ=255)                                                                         cATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGa                                                        <  1:154389/140‑1 (MQ=255)                                                                               aTGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACAt                                                  >  2:369837/1‑140 (MQ=255)                                                                               aTGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACAt                                                  >  1:294405/1‑140 (MQ=255)                                                                               aTGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGCTTCGAGGCCCAGCGACAGTGGGATCACAt                                                  >  2:47456/1‑140 (MQ=255)                                                                                 ggATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCa                                                >  1:52964/1‑140 (MQ=255)                                                                                 ggATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCa                                                <  2:661349/140‑1 (MQ=255)                                                                                   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGa                                                        <  1:625456/130‑1 (MQ=255)                                                                                   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGa                                                        >  2:625456/1‑130 (MQ=255)                                                                                   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAg                                              >  1:399307/1‑140 (MQ=255)                                                                                   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAg                                              >  1:19125/1‑140 (MQ=255)                                                                                            gCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCAt                                     >  2:658928/1‑140 (MQ=255)                                                                                             ccGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCAtt                                    >  2:892636/1‑140 (MQ=255)                                                                                                                ggtggtGAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAc                 <  2:19125/140‑1 (MQ=255)                                                                                                                 gtggtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc                <  1:47456/140‑1 (MQ=255)                                                                                                                  tggtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAccc               <  2:828185/140‑1 (MQ=255)                                                                                                                   ggtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGGCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTa                  <  1:718920/136‑1 (MQ=255)                                                                                                                   ggtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGGCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTa                  >  2:718920/1‑136 (MQ=255)                                                                                                                   ggtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACcca              >  1:111742/1‑140 (MQ=255)                                                                                                                   ggtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACcca              >  2:96971/1‑140 (MQ=255)                                                                                                                    gtgAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACccac             >  1:521136/1‑140 (MQ=255)                                                                                                                      gAAATCCTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACccacca           <  2:870686/140‑1 (MQ=255)                                                                                                                           ccTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAata      >  2:76010/1‑140 (MQ=255)                                                                                                                            cTGAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAatat     <  1:658928/140‑1 (MQ=255)                                                                                                                              gAGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATcc   >  2:72628/1‑140 (MQ=255)                                                                                                                               aGCGCGGGCCGCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCg  >  1:724672/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  TAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCG  >  NC_000913/2657550‑2657815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑