Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,416	C→T	A8A (GCG→GCA)	gshA ←	glutamate‑‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,416	0	C	T	100.0%	186.1 / NA	56	A8A (GCG→GCA)	gshA	glutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/30);  total (26/30)

GTCAGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGCGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGC  >  NC_000913/2816282‑2816550                                                                                                                                       |                                                                                                                                       gTCAGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCtg                                                                                                                                   >  1:238615/1‑140 (MQ=255)  tCAGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCtgt                                                                                                                                  >  2:577245/1‑140 (MQ=255)    aGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGa                                                                                                                                <  2:495138/140‑1 (MQ=255)      atcGGAACCTAATGCTTGACGACGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATa                                                                                                                              >  2:173064/3‑140 (MQ=255)        cGGAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACg                                                                                                                            >  2:324263/1‑140 (MQ=255)         ggAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGt                                                                                                                           <  1:147999/140‑1 (MQ=255)          gAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTc                                                                                                                          <  1:804740/140‑1 (MQ=255)              cTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCggg                                                                                                                      <  2:544280/140‑1 (MQ=255)                  tGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCa                                                                                                                  >  2:910788/1‑140 (MQ=255)                      tCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAAtt                                                                                                              <  1:577644/140‑1 (MQ=255)                      tCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAAtt                                                                                                              <  2:370949/140‑1 (MQ=255)                       cAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTg                                                                                                             >  1:192703/1‑140 (MQ=255)                        aGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCg                                                                                                                        >  2:907640/1‑128 (MQ=255)                        aGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCg                                                                                                                        <  1:907640/128‑1 (MQ=255)                              aCCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTccc                                                                                                      >  1:737634/1‑140 (MQ=255)                                aggttgttGCCAGTGTGCCATCAGCATTAACACGAAAAGTTCAGCGCTCCAGCCCACGCTGTATCCCCTTTACCGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTG‑CCCCCCGcc                                                                                                   <  2:848663/138‑1 (MQ=255)                                        gCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCaaa                                                                                            >  1:321883/1‑140 (MQ=255)                                        gCCAGTGTACCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCaaa                                                                                            >  2:434015/1‑140 (MQ=255)                                        cccAGTGTTCAACCAGCTTTACCCGGAAAAGTTTCGCGCTCCAGCCCACCCTGTATCGCCTTAAACCCCGGAGGTTGTTTTTCCACCCGGGCCTGTGCTTGTGATACGTCCGGGATCAAATTGACCCCCCGCCTGTCaaa                                                                                            <  2:827630/139‑1 (MQ=255)                                             tgtgCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGt                                                                                       >  2:725960/1‑140 (MQ=255)                                             tgtgCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGt                                                                                       >  2:776960/1‑140 (MQ=255)                                             tgtgCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGt                                                                                       >  1:31739/1‑140 (MQ=255)                                             tgtgCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACC‑CCCGCCTGTCAAAATCgct                                                                                      >  1:848663/1‑138 (MQ=255)                                             tgtgCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGATTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGt                                                                                       >  1:597499/1‑140 (MQ=255)                                                  cATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTaa                                                                                  <  2:321883/140‑1 (MQ=255)                                                   aTCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCTTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAAt                                                                                 <  1:784247/140‑1 (MQ=255)                                                   aTCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAAt                                                                                 >  1:739593/1‑140 (MQ=255)                                                      aGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAg                                                                              <  1:706767/140‑1 (MQ=255)                                                      aGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAg                                                                              <  1:577245/140‑1 (MQ=255)                                                       gCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGc                                                                             >  1:697755/1‑140 (MQ=255)                                                         aTTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCAt                                                                           >  2:200693/1‑140 (MQ=255)                                                         aTTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCAt                                                                           <  1:657704/140‑1 (MQ=255)                                                         aTTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCAt                                                                           <  2:739593/140‑1 (MQ=255)                                                         aTTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCAt                                                                           <  1:137052/140‑1 (MQ=255)                                                                  cAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCCTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTa                                                                                   <  2:660807/123‑1 (MQ=255)                                                                  cAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCCTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTa                                                                                   >  1:660807/1‑123 (MQ=255)                                                                   aaaGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATg                                                                 <  1:324263/140‑1 (MQ=255)                                                                   aaaGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATg                                                                 <  2:238615/140‑1 (MQ=255)                                                                      gTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTCCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTg                                                              <  1:725960/140‑1 (MQ=255)                                                                        ttCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGt                                                               <  1:626896/137‑1 (MQ=255)                                                                        ttCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGt                                                               >  2:626896/1‑137 (MQ=255)                                                                                ccAGCCCACGCTGTATCCCTTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATAtgtg                                                    <  2:31739/140‑1 (MQ=255)                                                                                ccAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATAtgtg                                                    <  2:18950/140‑1 (MQ=255)                                                                                      cACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGCCCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGcc                                              <  1:7062/140‑1 (MQ=255)                                                                                            gTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAAt                                        <  1:200693/140‑1 (MQ=255)                                                                                               tCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAg                                     <  1:391518/140‑1 (MQ=255)                                                                                                 cccTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCa                                                  >  2:769584/1‑125 (MQ=255)                                                                                                 cccTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCa                                                  <  1:769584/125‑1 (MQ=255)                                                                                                 cccTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTg                                   <  1:776960/140‑1 (MQ=255)                                                                                                     ttAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGccac                               >  1:672434/1‑140 (MQ=255)                                                                                                     ttAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGccac                               >  2:697496/1‑140 (MQ=255)                                                                                                     ttAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGccac                               >  1:910016/1‑140 (MQ=255)                                                                                                      tagcGCCTGAGGAGGTTTTTCCAGCCAGGCCAGTGCCGGTGAAACGTCCGGGATCAAATTAACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGccacc                              <  2:597499/137‑1 (MQ=255)                                                                                                      tAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGccacc                              <  1:703684/140‑1 (MQ=255)                                                                                                         cGCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACcaca                           >  2:97345/1‑140 (MQ=255)                                                                                                          gCCTGAGGATGTTTTTCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGCCCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCAcat                          <  1:669034/140‑1 (MQ=255)                                                                                                                     tttttCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGaa               >  2:481438/1‑140 (MQ=255)                                                                                                                       tttCCAGCCAGGCCAGTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACg             <  1:697496/140‑1 (MQ=255)                                                                                                                                  gccagTGCCTGTGATACGTCCGGGATCAAATTGCCCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCtgctgc  <  1:910788/140‑1 (MQ=255)                                                                                                                                  gccagTGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCtgctgc  >  1:242427/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GTCAGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTTGCCAGTGTGCCATCAGCATTAACACGCAAAGTTTCGCGCTCCAGCCCACGCTGTATCCCCTTTAACGCCTGAGGATGTTTTTCCAGCCAGGCCAGCGCCTGTGATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGC  >  NC_000913/2816282‑2816550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑