Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,722	G→A	G111S (GGC→AGC)	srlE →	sorbitol‑specific PTS enzyme IIBC1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,722	0	G	A	100.0%	206.8 / NA	58	G111S (GGC→AGC)	srlE	sorbitol‑specific PTS enzyme IIBC1 component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/30);  total (28/30)

GTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG  >  NC_000913/2826585‑2826858                                                                                                                                          |                                                                                                                                         gtgtCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGc                                                                                                                                        >  2:337543/1‑140 (MQ=255)   gtCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGt                                                                                                                                      >  1:712384/1‑140 (MQ=255)     cgcgGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTaa                                                                                                                                    >  2:607865/1‑140 (MQ=255)       cgGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTaaaa                                                                                                                                  <  1:551227/140‑1 (MQ=255)        ggTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAaag                                                                                                                                 <  1:895686/140‑1 (MQ=255)         gTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTaaaa                                                                                                                                  >  2:725115/1‑138 (MQ=255)         gTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTaaaa                                                                                                                                  <  1:725115/138‑1 (MQ=255)                   gtgGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATc                                                                                                                      <  2:137246/140‑1 (MQ=255)                   gtgGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATc                                                                                                                      <  1:337543/140‑1 (MQ=255)                    tgGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCa                                                                                                                     <  1:257643/140‑1 (MQ=255)                    tgGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCa                                                                                                                     >  1:514700/1‑140 (MQ=255)                      gcggcACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACt                                                                                                                   <  1:216569/140‑1 (MQ=255)                                      cGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGa                                                                                                   <  2:17081/140‑1 (MQ=255)                                      cGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGa                                                                                                   >  1:660538/1‑140 (MQ=255)                                        gCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGaca                                                                                                 <  1:13860/140‑1 (MQ=255)                                           tCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGAcacca                                                                                              >  2:193295/1‑140 (MQ=255)                                             tATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACcaca                                                                                            <  2:138515/140‑1 (MQ=255)                                             tATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACcaca                                                                                            <  2:133808/140‑1 (MQ=255)                                                   aaaCGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGa                                                                                                   <  2:339222/127‑1 (MQ=255)                                                   aaaCGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGa                                                                                                   >  1:339222/1‑127 (MQ=255)                                                         cgTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCct                                                                                       >  1:273286/1‑133 (MQ=255)                                                         cgTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCct                                                                                       <  2:273286/133‑1 (MQ=255)                                                         cgTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                >  2:13420/1‑140 (MQ=255)                                                              tCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                           <  2:712384/140‑1 (MQ=255)                                                                ccaccaTTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                         <  2:622066/140‑1 (MQ=255)                                                                 caccaTTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                        <  2:246285/140‑1 (MQ=255)                                                                     aTTAATATCCACTCGACGGGCAAGTCCGGTCCACTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                    >  2:195883/1‑140 (MQ=255)                                                                       tAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                  <  2:514700/140‑1 (MQ=255)                                                                              cACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                           <  2:127553/124‑1 (MQ=255)                                                                              cACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                           >  1:127553/1‑124 (MQ=255)                                                                               aCTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATGTTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                          >  2:332330/1‑140 (MQ=255)                                                                               aCTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                          >  1:696755/1‑140 (MQ=255)                                                                                 tCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                        >  1:704399/1‑140 (MQ=255)                                                                                 tCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                        >  2:388685/1‑140 (MQ=255)                                                                                                  tccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                       >  1:132456/1‑140 (MQ=255)                                                                                                  tccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                       >  2:128076/1‑140 (MQ=255)                                                                                                      cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                   <  1:128076/140‑1 (MQ=255)                                                                                                      cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                   <  2:132456/140‑1 (MQ=255)                                                                                                       tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                  >  1:25726/1‑140 (MQ=255)                                                                                                         gcgcAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAGTCACCGAACAAAGCGATgg                                >  1:597133/1‑140 (MQ=255)                                                                                                            cAGTACATTGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt                             <  1:388685/140‑1 (MQ=255)                                                                                                                   ttGTGGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                      <  1:193295/140‑1 (MQ=255)                                                                                                                      tgGAAGATGTTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                   <  1:332330/140‑1 (MQ=255)                                                                                                                      tgGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                   >  1:598428/1‑140 (MQ=255)                                                                                                                      tgGAAGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                   >  2:701390/1‑140 (MQ=255)                                                                                                                         aaGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                >  2:208072/1‑140 (MQ=255)                                                                                                                         aaGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                >  2:386954/1‑140 (MQ=255)                                                                                                                         aaGATATTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                >  2:167255/1‑140 (MQ=255)                                                                                                                             tatTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa            <  1:669833/140‑1 (MQ=255)                                                                                                                             tatTTATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa            <  2:598428/140‑1 (MQ=255)                                                                                                                                ttATGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg         >  2:801270/1‑140 (MQ=255)                                                                                                                                   tGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa      >  1:22744/1‑140 (MQ=255)                                                                                                                                   tGTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa      <  2:660538/140‑1 (MQ=255)                                                                                                                                    gTCTCTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt     <  1:13420/140‑1 (MQ=255)                                                                                                                                     tctcTAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg    <  1:386954/140‑1 (MQ=255)                                                                                                                                       tctAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt                 <  1:875236/125‑1 (MQ=255)                                                                                                                                       tctAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg  <  1:607865/140‑1 (MQ=255)                                                                                                                                       tctAGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg  >  2:831318/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         GTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG  >  NC_000913/2826585‑2826858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑