| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,855,509 | T→C | V391A (GTG→GCG) | fhlA → | DNA‑binding transcriptional activator FhlA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,855,509 | 0 | T | C | 98.4% | 212.3 / ‑4.6 | 61 | V391A (GTG→GCG) | fhlA | DNA‑binding transcriptional activator FhlA |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (28/32); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGA > NC_000913/2855378‑2855646 | ccTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAAc > 1:744597/1‑140 (MQ=255)ccTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAAc < 2:730984/140‑1 (MQ=255) tCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACaa > 1:583764/1‑140 (MQ=255) aaTCCATCGTCTGAAAGTACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAAtggtgg < 2:456488/140‑1 (MQ=255) aaTCCAACGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAAtggtgg > 2:271519/1‑140 (MQ=255) aTCCATCGACTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGgc > 1:293546/1‑140 (MQ=255) aTCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCaa < 2:914419/140‑1 (MQ=255) aTCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCaa < 1:791537/140‑1 (MQ=255) tCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCaaa > 1:339694/1‑140 (MQ=255) tCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCaaa > 2:518408/1‑140 (MQ=255) tCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCaa > 1:7586/1‑139 (MQ=255) tCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCaa < 2:7586/139‑1 (MQ=255) cGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGTCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAg > 2:797298/1‑140 (MQ=255) tCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAGTTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTg > 2:408614/1‑140 (MQ=255) cTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGa < 2:153851/140‑1 (MQ=255) cTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGa < 2:583764/140‑1 (MQ=255) aGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAGTTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTa < 1:408614/140‑1 (MQ=255) aaCGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTAcc < 1:525292/140‑1 (MQ=255) aaCGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTAcc < 1:525613/140‑1 (MQ=255) cGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCg < 2:175272/139‑1 (MQ=255) cGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCg > 1:175272/1‑139 (MQ=255) ttGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATc < 2:54916/140‑1 (MQ=255) aaaaCCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGt < 2:313351/140‑1 (MQ=255) cccTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGc < 1:97724/140‑1 (MQ=255) cccTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGc > 2:88147/1‑140 (MQ=255) aCCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACggg > 2:866293/1‑140 (MQ=255) cGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTa < 1:271519/140‑1 (MQ=255) agcagcTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGCTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCa > 1:297517/1‑132 (MQ=255) agcagcTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGCTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCa < 2:297517/132‑1 (MQ=255) gcagcTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAag < 1:446618/140‑1 (MQ=255) cagcTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTgg > 1:747301/1‑128 (MQ=255) cagcTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTgg < 2:747301/128‑1 (MQ=255) tCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTg < 1:866293/140‑1 (MQ=255) tGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGccc < 1:88147/140‑1 (MQ=255) gTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCg < 2:820315/140‑1 (MQ=255) ttGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGAt < 2:498367/133‑1 (MQ=255) ttGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGAt > 1:498367/1‑133 (MQ=255) ttGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGc > 2:127691/1‑136 (MQ=255) ttGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGc < 1:127691/136‑1 (MQ=255) ttGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGt > 1:405246/1‑140 (MQ=255) ttGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGt > 2:136109/1‑140 (MQ=255) gAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTg > 2:401744/1‑133 (MQ=255) gAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTg < 1:401744/133‑1 (MQ=255) gAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCa > 2:415481/1‑140 (MQ=255) aaTTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGAt < 2:603902/124‑1 (MQ=255) aaTTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGAt > 1:603902/1‑124 (MQ=255) aTTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGAt > 2:479322/1‑123 (MQ=255) aTTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGAt < 1:479322/123‑1 (MQ=255) tGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAAtc > 1:631866/1‑140 (MQ=255) cGCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGtaataa > 1:262606/1‑140 (MQ=255) gCAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTaataat > 2:68071/1‑140 (MQ=255) cAGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATc > 1:622065/1‑140 (MQ=255) aGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTgg < 1:733985/128‑1 (MQ=255) aGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTgg > 2:520204/1‑128 (MQ=255) aGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTgg > 2:733985/1‑128 (MQ=255) aGCGAAGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTgg < 1:520204/128‑1 (MQ=255) aGCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCa < 1:136109/140‑1 (MQ=255) gCCATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCAt < 2:622065/140‑1 (MQ=255) cATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATgg < 1:68071/140‑1 (MQ=255) cATGTACAGCGCGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATgg < 2:405246/140‑1 (MQ=255) cGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTTGGCGTAATAATCGCCGCATGGTCAAAATGa > 2:237122/1‑140 (MQ=255) | CCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGA > NC_000913/2855378‑2855646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |