Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,955,650	T→C	E118E (GAA→GAG)	recB ←	exodeoxyribonuclease V subunit RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,955,650	0	T	C	100.0%	201.4 / NA	56	E118E (GAA→GAG)	recB	exodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

GTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGTTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGCTA  >  NC_000913/2955521‑2955773                                                                                                                                  |                                                                                                                            gtagcgtagcAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTaaca                                                                                                                   >  2:813283/1‑140 (MQ=255)           agagACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTg                                                                                                         >  2:24813/1‑140 (MQ=255)                    tCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTg                                                                                                <  1:24813/140‑1 (MQ=255)                       tCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTgcgc                                                                                             >  2:765995/1‑140 (MQ=255)                         aaTCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCtt                                                                                           >  2:422724/1‑140 (MQ=255)                               ctgctgctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTAtcgt                                                                                     >  1:853267/1‑140 (MQ=255)                               ctgctgctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTAtcgt                                                                                     >  1:3148/1‑140 (MQ=255)                               ctgctgctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTAtcgt                                                                                     >  1:567446/1‑140 (MQ=255)                               ctgctgctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTAtcgt                                                                                     <  1:546908/140‑1 (MQ=255)                                  ctgctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTa                                                                                         <  1:466682/133‑1 (MQ=255)                                  ctgctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTa                                                                                         >  2:466682/1‑133 (MQ=255)                                    gctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGAtc                                                                                <  1:422724/140‑1 (MQ=255)                                    gctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGAtc                                                                                >  2:876992/1‑140 (MQ=255)                                     ctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGAtct                                                                               >  1:328323/1‑140 (MQ=255)                                     ctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGAtct                                                                               <  1:733311/140‑1 (MQ=255)                                     ctCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGAtct                                                                               <  2:39965/140‑1 (MQ=255)                                              cATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCcagca                                                                      <  1:800916/140‑1 (MQ=255)                                               aTGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTTGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCcagcag                                                                     >  1:293883/1‑140 (MQ=255)                                                tGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAgg                                                                    >  1:555688/1‑140 (MQ=255)                                                 gCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGc                                                                   <  1:876992/140‑1 (MQ=255)                                                   cGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGt                                                                 <  2:528568/140‑1 (MQ=255)                                                    ggATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGtt                                                                <  1:813283/140‑1 (MQ=255)                                                     gATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTc                                                               <  1:464589/140‑1 (MQ=255)                                                      aTTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCg                                                              >  1:220768/1‑140 (MQ=255)                                                        tCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTa                                                            <  2:220768/140‑1 (MQ=255)                                                        tCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTa                                                            <  1:747001/140‑1 (MQ=255)                                                          aaaGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACa                                                          <  2:474953/140‑1 (MQ=255)                                                            aGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAgg                                                                    <  1:320235/128‑1 (MQ=255)                                                            aGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAgg                                                                    >  2:320235/1‑128 (MQ=255)                                                                        tGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCggtg                                            >  1:491440/1‑140 (MQ=255)                                                                               gcgcTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCAc                                     >  2:905943/1‑140 (MQ=255)                                                                                          aGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCg                          <  2:3148/140‑1 (MQ=255)                                                                                          aGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCg                          <  2:567446/140‑1 (MQ=255)                                                                                               tGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATgcg                     >  1:824934/1‑140 (MQ=255)                                                                                               tGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATgcg                     <  2:731722/140‑1 (MQ=255)                                                                                               tGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCAATgcg                     >  1:746438/1‑140 (MQ=255)                                                                                                gAATAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCAATgcgc                    <  2:746438/140‑1 (MQ=255)                                                                                                  aTAGTAAAGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCaa                  <  2:824934/140‑1 (MQ=255)                                                                                                        aaGACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTg            <  2:555688/140‑1 (MQ=255)                                                                                                          gACTGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCAATGCGCGGCTTGCGCTTTATCGTCGATCTCTTCTAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGa          <  2:853267/140‑1 (MQ=255)                                                                                                             tGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCaa                  >  1:23809/1‑129 (MQ=255)                                                                                                             tGCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCaa                  <  2:23809/129‑1 (MQ=255)                                                                                                              gCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTc               <  2:795566/131‑1 (MQ=255)                                                                                                              gCCGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTc               >  1:795566/1‑131 (MQ=255)                                                                                                               ccGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGata        <  2:595706/137‑1 (MQ=255)                                                                                                               ccGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGata        >  1:595706/1‑137 (MQ=255)                                                                                                               ccGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTg     >  2:712101/1‑140 (MQ=255)                                                                                                               ccGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTg     >  1:361529/1‑140 (MQ=255)                                                                                                               ccGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTg     >  1:155605/1‑140 (MQ=255)                                                                                                                cGCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGc    >  2:438511/1‑140 (MQ=255)                                                                                                                 gCTTCATCCATCTGCCGCTTGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTAGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGCt   <  2:293883/140‑1 (MQ=255)                                                                                                                 gCTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGCt   <  2:361529/140‑1 (MQ=255)                                                                                                                  cTTCATCCATCTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGCTa  >  2:737521/1‑140 (MQ=255)                                                                                                                     catccatcTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGatat       >  1:345566/1‑132 (MQ=255)                                                                                                                     catccatcTGCCGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGatat       <  2:345566/132‑1 (MQ=255)                                                                                                                               ccGCTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGCt   <  1:867571/126‑1 (MQ=255)                                                                                                                                  |                                                                                                                            GTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTCATCCATCTGCCGTTCGGCTAACAACAACCACTGCGCGGCTTGCGCTTTATCGTCGATCTCTTCCAGCAGGCGTTCGTACAGTGGATTGTCGGTGGTTTCACGCAGACAGGCGATGCGCAACTCGTGGATATTGCTA  >  NC_000913/2955521‑2955773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑