Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,020,895	T→C	V119A (GTC→GCC)	ygfM →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,895	0	T	C	100.0%	157.4 / NA	44	V119A (GTC→GCC)	ygfM	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/26);  total (18/26)

CACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTATCGCACCTGTG  >  NC_000913/3020760‑3021023                                                                                                                                        |                                                                                                                                 cACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTg                                                                                                                              <  2:218698/140‑1 (MQ=255)  ccTGGGTGATGCGGGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGt                                                                                                                             <  2:596778/139‑1 (MQ=255)      cGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGc                                                                                                                         <  2:756649/140‑1 (MQ=255)      cGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGc                                                                                                                         >  2:241112/1‑140 (MQ=255)        tGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCAc                                                                                                                       >  2:338844/1‑140 (MQ=255)         gATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACt                                                                                                                      <  1:179761/140‑1 (MQ=255)         gATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACt                                                                                                                      <  2:694840/140‑1 (MQ=255)             cgcgATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGAt                                                                                                                  >  2:917276/1‑140 (MQ=255)              gcgATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATg                                                                                                                 <  2:752321/140‑1 (MQ=255)                                gcTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCa                                                                                               <  2:289130/140‑1 (MQ=255)                                 ctgagggAAGCCCTCGGTTTTGTTTACTCACGCGATGTTCGTACTCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCaa                                                                                              <  2:606110/134‑1 (MQ=255)                                 cTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCaa                                                                                              <  1:82193/140‑1 (MQ=255)                                 cTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCaa                                                                                              >  1:334590/1‑140 (MQ=255)                                       gAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGa                                                                                        >  1:562639/1‑140 (MQ=255)                                              tCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGc                                                                                          >  2:333875/1‑131 (MQ=255)                                              tCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGc                                                                                          <  1:333875/131‑1 (MQ=255)                                               cGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGt                                                                                <  2:562639/140‑1 (MQ=255)                                               cGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGt                                                                                <  2:441477/140‑1 (MQ=255)                                                  ttttGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCaa                                                                             <  1:917276/140‑1 (MQ=255)                                                    ttGTTTACTCACGCCATGTTCTTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATc                                                                           <  2:835308/140‑1 (MQ=255)                                                       tttACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAg                                                                        >  2:335111/1‑140 (MQ=255)                                                        ttACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAgg                                                                       <  2:189297/140‑1 (MQ=255)                                                        ttACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAgg                                                                       <  1:494822/140‑1 (MQ=255)                                                            tCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTa                                                                   >  2:14091/1‑140 (MQ=255)                                                             cACGCCATGTTTGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCACGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTAc                                                                  <  1:614917/140‑1 (MQ=255)                                                                gCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTg                                                               <  2:38027/140‑1 (MQ=255)                                                                     gTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTg                                                          >  2:147131/1‑140 (MQ=255)                                                                        cGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGccc                                                       <  2:334590/140‑1 (MQ=255)                                                                               aGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGAt                                                <  1:14091/140‑1 (MQ=255)                                                                                gTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATc                                               <  2:132387/140‑1 (MQ=255)                                                                                gTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATc                                               >  1:879915/1‑140 (MQ=255)                                                                                 tCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCg                                              >  1:895345/1‑140 (MQ=255)                                                                                 tCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCg                                              >  2:763372/1‑140 (MQ=255)                                                                                           gtggtgAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAAcc                                    >  1:226359/1‑140 (MQ=255)                                                                                             ggtgAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGa                                  >  1:798295/1‑140 (MQ=255)                                                                                               tgAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGaaa                                >  2:447697/1‑140 (MQ=255)                                                                                                   aTCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTAt                            <  1:241112/140‑1 (MQ=255)                                                                                                     cgccgcCCGCCAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCa                          <  2:895345/140‑1 (MQ=255)                                                                                                        cgcccgccAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTa                       <  2:226359/140‑1 (MQ=255)                                                                                                        cgcccgccAGGAAGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTa                       <  1:447697/140‑1 (MQ=255)                                                                                                                   aaGAGTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTAt            <  1:763372/140‑1 (MQ=255)                                                                                                                      agTCGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTCAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTATCGc         >  2:496786/1‑140 (MQ=255)                                                                                                                         cGGTGCTGCTTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTATCGCAcc      >  1:240408/1‑140 (MQ=255)                                                                                                                             gctgctTCCCGCCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTATCGCACCtgtg  >  2:337812/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                 CACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTATCGCACCTGTG  >  NC_000913/3020760‑3021023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑