Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,906	T→C	V307A (GTC→GCC)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,906	0	T	C	100.0%	223.2 / NA	62	V307A (GTC→GCC)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/35);  total (27/35)

GCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCA  >  NC_000913/3063770‑3064041                                                                                                                                         |                                                                                                                                        gCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCaa                                                                                                                                      <  1:914617/140‑1 (MQ=255)         tCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATa                                                                                                                              <  1:2152/140‑1 (MQ=255)            tttACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGc                                                                                                                           >  1:549520/1‑140 (MQ=255)                caacaaGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCtctc                                                                                                                       >  2:885209/1‑140 (MQ=255)                   caaGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCAcc                                                                                                                    <  1:761003/140‑1 (MQ=255)                   caaGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCAcc                                                                                                                    >  2:113017/1‑140 (MQ=255)                      gTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACcgcg                                                                                                                 >  1:638560/1‑140 (MQ=255)                       tGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCgcgc                                                                                                                >  2:282991/1‑140 (MQ=255)                       tGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCgcgc                                                                                                                >  2:173975/1‑140 (MQ=255)                       tGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCgcgc                                                                                                                >  1:42531/1‑140 (MQ=255)                       tGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCgcgc                                                                                                                >  1:670006/1‑140 (MQ=255)                       tGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCgcgc                                                                                                                >  1:53036/1‑140 (MQ=255)                        gCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCa                                                                                                               <  2:670006/140‑1 (MQ=255)                        gCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCa                                                                                                               <  1:22340/140‑1 (MQ=255)                        gCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCa                                                                                                               <  2:550380/140‑1 (MQ=255)                         cGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCAc                                                                                                              <  1:885209/140‑1 (MQ=255)                         cGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCAc                                                                                                              <  2:871872/140‑1 (MQ=255)                         cGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCAc                                                                                                              <  1:282991/140‑1 (MQ=255)                              acaacaaTCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGcc                                                                                                         <  2:470927/140‑1 (MQ=255)                                          ggAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCa                                                                                             >  1:402701/1‑140 (MQ=255)                                           gAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAg                                                                                            <  1:479206/140‑1 (MQ=255)                                             aTGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTg                                                                                          >  1:640678/1‑140 (MQ=255)                                             aTGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTg                                                                                          >  1:187004/1‑140 (MQ=255)                                                 cTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAAtt                                                                                      >  1:438655/1‑140 (MQ=255)                                                   gCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTa                                                                                    >  1:707855/1‑140 (MQ=255)                                                     gTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATc                                                                                  >  2:196873/1‑140 (MQ=255)                                                       aaGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCa                                                                                <  1:370696/140‑1 (MQ=255)                                                        aGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAg                                                                               >  2:566818/1‑140 (MQ=255)                                                          cAGACCGAAGAAGAAGTACTGACTCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATcc                                                                                 <  1:641006/136‑1 (MQ=255)                                                          cAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATcc                                                                                 >  2:641006/1‑136 (MQ=255)                                                            gACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGc                                                                           <  1:566818/140‑1 (MQ=255)                                                            gACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGc                                                                           <  2:53036/140‑1 (MQ=255)                                                                       aagTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGAt                                                                >  2:879610/1‑140 (MQ=255)                                                                       aagTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGAt                                                                >  1:103127/1‑140 (MQ=255)                                                                         gTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATAtt                                                                     >  1:517916/1‑133 (MQ=255)                                                                         gTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATAtt                                                                     <  2:517916/133‑1 (MQ=255)                                                                           aCTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTaaa                                                            >  2:273512/1‑140 (MQ=255)                                                                              gAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGa                                                         <  1:712054/140‑1 (MQ=255)                                                                              gAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGa                                                         <  1:173975/140‑1 (MQ=255)                                                                               aaTCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGaa                                                        >  1:920775/1‑140 (MQ=255)                                                                                 tCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAAtt                                                      >  1:169507/1‑140 (MQ=255)                                                                                   aCCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAAtttt                                                    <  2:920775/140‑1 (MQ=255)                                                                                        ttCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGt                                               <  1:113017/140‑1 (MQ=255)                                                                                         tCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTc                                              <  2:638560/140‑1 (MQ=255)                                                                                           gcgAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCtt                                            >  2:662304/1‑140 (MQ=255)                                                                                           gcgAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCtt                                            <  2:103127/140‑1 (MQ=255)                                                                                            cgAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTa                                           <  2:549520/140‑1 (MQ=255)                                                                                            cgAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTa                                           <  2:88551/140‑1 (MQ=255)                                                                                             gAATGAAGATCTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTAt                                          <  1:860749/140‑1 (MQ=255)                                                                                              aaTGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATc                                         <  1:857229/140‑1 (MQ=255)                                                                                              aaTGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATc                                         <  2:434861/140‑1 (MQ=255)                                                                                               aTGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCa                                        <  2:187004/140‑1 (MQ=255)                                                                                               aTGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCa                                        <  2:42531/140‑1 (MQ=255)                                                                                                tGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAg                                       <  1:273512/140‑1 (MQ=255)                                                                                                        tCGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTaa                               <  1:879610/140‑1 (MQ=255)                                                                                                         cGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTAt                                          <  1:712668/128‑1 (MQ=255)                                                                                                         cGATCGCTATTACCGCCAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTAt                                          >  2:712668/1‑128 (MQ=255)                                                                                                                         cAGACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATcaaca              <  1:662304/140‑1 (MQ=255)                                                                                                                           gACGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACaaa            >  2:682813/1‑140 (MQ=255)                                                                                                                            aCGCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAg           >  2:499645/1‑140 (MQ=255)                                                                                                                              gCTTTTAGCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTg         <  1:196873/140‑1 (MQ=255)                                                                                                                                     gCGGCCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCa  <  2:707855/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCA  >  NC_000913/3063770‑3064041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑