Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,219	(C)6→5	coding (200/672 nt)	queE ←	putative 7‑carboxy‑7‑deazaguanine synthase QueE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,214	0	C	.	100.0%	357.3 / NA	84	coding (205/672 nt)	queE	putative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (51/33);  total (51/33)

CAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAAT  >  NC_000913/2905075‑2905349                                                                                                                                            |                                                                                                                                         cAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGc                                                                                                                                          <  1:764076/140‑1 (MQ=255) cAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGc                                                                                                                                          <  2:339096/140‑1 (MQ=255) cAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGc                                                                                                                                          <  1:905783/140‑1 (MQ=255)     tAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGccccc                                                                                                                                      <  2:238430/140‑1 (MQ=255)          ccGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTa                                                                                                                                >  2:879460/1‑140 (MQ=255)          ccGTTCTTTTCCAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTa                                                                                                                                >  1:1219002/1‑140 (MQ=255)            gTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATc                                                                                                                              <  1:214425/140‑1 (MQ=255)             ttCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCa                                                                                                                             >  1:188165/1‑140 (MQ=255)             ttCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCATAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGcc                                                                                                                                         >  2:1532276/1‑129 (MQ=255)             ttCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCATAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGcc                                                                                                                                         <  1:1532276/129‑1 (MQ=255)              tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCAc                                                                                                                            <  2:1329048/140‑1 (MQ=255)              tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCAc                                                                                                                            <  2:1557589/140‑1 (MQ=255)              tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCAc                                                                                                                            >  2:499055/1‑140 (MQ=255)                    cGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCtt                                                                                                                      <  1:1151689/140‑1 (MQ=255)                        cAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑t                                                                                                                 <  2:842508/140‑1 (MQ=255)                          gATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCtt                                                                                                                      >  2:616008/1‑134 (MQ=255)                          gATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCtt                                                                                                                      <  1:616008/134‑1 (MQ=255)                                tcagtGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACAACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCa                                                                                                         <  2:657588/140‑1 (MQ=255)                                    tGGCAGCAAATCATGAATGCAAGGCTCAGCACCCGTAATCACCACATGCTGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAAt                                                                                                     >  1:1421194/1‑140 (MQ=255)                                    tGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAAt                                                                                                     >  2:1230493/1‑140 (MQ=255)                                    tGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAAt                                                                                                     >  1:200117/1‑140 (MQ=255)                                     ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATg                                                                                                    >  1:356498/1‑140 (MQ=255)                                     ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATg                                                                                                    >  2:1412463/1‑140 (MQ=255)                                     ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATg                                                                                                    >  2:390069/1‑140 (MQ=255)                                      gcagcaAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGc                                                                                                   <  1:345802/140‑1 (MQ=255)                                         gcaAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGa                                                                                                >  2:1413543/1‑140 (MQ=255)                                          caAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGaa                                                                                               >  2:1566341/1‑140 (MQ=255)                                             aTCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAg                                                                                            >  1:820358/1‑140 (MQ=255)                                                   aaTGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGAc                                                                                      <  2:820358/140‑1 (MQ=255)                                                    aTGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACt                                                                                     >  2:605279/1‑140 (MQ=255)                                                         aGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCg                                                                                >  2:25307/1‑140 (MQ=255)                                                           gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGAt                                                                              >  1:812411/1‑140 (MQ=255)                                                               accaccCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTc                                                                          >  2:88243/1‑140 (MQ=255)                                                                  ccccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                                                                       <  2:72785/139‑1 (MQ=255)                                                                  accCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                                                                       <  2:1277844/140‑1 (MQ=255)                                                                   cccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGc                                                                      <  2:1063896/140‑1 (MQ=255)                                                                      gTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGGTTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCtt                                                                    >  2:1500025/1‑140 (MQ=255)                                                                      gTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCttt                                                                   >  2:1333347/1‑140 (MQ=255)                                                                       tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCtttt                                                                  >  2:1322514/1‑140 (MQ=255)                                                                        aaTCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTc                                                                 <  1:1566341/140‑1 (MQ=255)                                                                           caccacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCa                                                              >  2:1555376/1‑140 (MQ=255)                                                                           caccacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCa                                                              >  2:1214082/1‑140 (MQ=255)                                                                            accacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAg                                                             <  1:88243/140‑1 (MQ=255)                                                                            accacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAg                                                             <  1:105652/140‑1 (MQ=255)                                                                              cacaTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt                                                           <  2:812411/140‑1 (MQ=255)                                                                                   gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGtt                                                      <  1:1333347/140‑1 (MQ=255)                                                                                     cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTg                                                    >  2:243479/1‑140 (MQ=255)                                                                                      gcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTgg                                                   >  1:1241439/1‑140 (MQ=255)                                                                                       cgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgt                                                  >  1:925391/1‑140 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 >  1:1033781/1‑140 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 <  1:955745/140‑1 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 <  1:879460/140‑1 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 <  1:1417803/140‑1 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 <  1:243479/140‑1 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 <  1:351753/140‑1 (MQ=255)                                                                                        gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCAATCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg                                                 >  1:1403306/1‑140 (MQ=255)                                                                                           gtgtATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCg                                              >  1:1136602/1‑140 (MQ=255)                                                                                             gtATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCa                                            <  2:356498/140‑1 (MQ=255)                                                                                              tATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCAc                                           <  2:1414941/140‑1 (MQ=255)                                                                                                     ggCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGcac                                    >  2:453838/1‑140 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTc                                               <  2:1262112/128‑1 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTc                                               >  1:1262112/1‑128 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGtaca                                   >  2:1036302/1‑140 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGcaca                                   >  2:101791/1‑140 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGcaca                                   >  1:1067542/1‑140 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGcaca                                   >  1:719612/1‑140 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTATGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTc                                               >  2:1481659/1‑128 (MQ=255)                                                                                                      gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTATGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTc                                               <  1:1481659/128‑1 (MQ=255)                                                                                                        gACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGc                                 >  1:1194482/1‑140 (MQ=255)                                                                                                          ccAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAg                                        >  2:1569614/1‑131 (MQ=255)                                                                                                          ccAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAg                                        <  1:1569614/131‑1 (MQ=255)                                                                                                          ccAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCa                               >  2:817244/1‑140 (MQ=255)                                                                                                          ccAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCa                               >  1:663663/1‑140 (MQ=255)                                                                                                          ccAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCa                               >  1:80182/1‑140 (MQ=255)                                                                                                           cAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCaa                              <  2:1241439/140‑1 (MQ=255)                                                                                                           cAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCaa                              >  2:1377288/1‑140 (MQ=255)                                                                                                            aaTGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAAc                             >  1:349464/1‑140 (MQ=255)                                                                                                                aCAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCggg                         >  1:790977/1‑140 (MQ=255)                                                                                                                    ccagcAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATc                     >  2:1331041/1‑140 (MQ=255)                                                                                                                     cagcAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATcc                    >  1:498603/1‑140 (MQ=255)                                                                                                                     cagcAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATcc                    <  1:627805/140‑1 (MQ=255)                                                                                                                      agcAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATccc                   >  1:687897/1‑140 (MQ=255)                                                                                                                      agcAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATccc                   >  2:333124/1‑140 (MQ=255)                                                                                                                      agcAAATCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATccc                   >  1:479080/1‑140 (MQ=255)                                                                                                                          aaTCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTa               <  2:790977/140‑1 (MQ=255)                                                                                                                           aTCTTCACTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGTACAGCCAACCGGGCATCCCTGTaa              <  1:1036302/140‑1 (MQ=255)                                                                                                                                 aCTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGGTTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGaa         <  1:1500025/140‑1 (MQ=255)                                                                                                                                  cTGCTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATa       <  1:390069/140‑1 (MQ=255)                                                                                                                                     cTCGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATaaaa    >  1:126770/1‑140 (MQ=255)                                                                                                                                       cGCAG‑CCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAAt  >  1:936918/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                         CAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCACTTATCACTCTCTTTGG‑TCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAAT  >  NC_000913/2905075‑2905349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑