BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,765,794	(C)_7→8	intergenic (‑18/‑124)	yfjM ← / → rnlA	CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,765,787	1	.	C	100.0%	374.8 / NA	107	intergenic (‑11/‑131)	yfjM/rnlA	CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (39/68); total (39/68)

Only 100 of 120 total aligned reads displayed.

TGGGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAA  >  NC_000913/2765648‑2765918

tGGGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTg                                                                                                                                       <  2:195317/140‑1 (MQ=255)

  ggCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cc                                                                                                                                   >  1:1539145/1‑140 (MQ=255)

  ggCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cc                                                                                                                                   >  1:1204136/1‑140 (MQ=255)

    ctctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccc                                                                                                                                 <  2:1204136/140‑1 (MQ=255)

    ctctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccc                                                                                                                                 <  2:211535/140‑1 (MQ=255)

     tctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccc                                                                                                                                <  1:724696/140‑1 (MQ=255)

     tctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccc                                                                                                                                <  2:901652/140‑1 (MQ=255)

     tctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccc                                                                                                                                <  2:806712/140‑1 (MQ=255)

       tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccccc                                                                                                                              >  2:960599/1‑140 (MQ=255)

       tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccccc                                                                                                                              >  1:153355/1‑140 (MQ=255)

       tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccccc                                                                                                                              <  2:568072/140‑1 (MQ=255)

              gTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGa                                                                                                                        <  2:825748/140‑1 (MQ=255)

              gTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGa                                                                                                                        <  1:767982/140‑1 (MQ=255)

              gTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGa                                                                                                                        <  1:1333372/140‑1 (MQ=255)

                 cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGa                                                                                                                     >  2:939551/1‑140 (MQ=255)

                 cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGa                                                                                                                     <  1:806321/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATc                                                                                                                   <  2:21256/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATc                                                                                                                   <  1:1551777/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATc                                                                                                                   <  1:960599/140‑1 (MQ=255)

                      tCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTg                                                                                                                >  1:667326/1‑140 (MQ=255)

                         tcGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                             <  1:1107821/139‑1 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                             <  1:1404734/140‑1 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                             >  1:379123/1‑140 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                             <  1:1235064/140‑1 (MQ=255)

                          cGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCt                                                                                                            >  2:703383/1‑140 (MQ=255)

                          cGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCGCCCCGGTAGACGATCCTGCCCta                                                                                                           <  2:148060/140‑1 (MQ=255)

                           gTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCta                                                                                                           <  2:884848/140‑1 (MQ=255)

                           gTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCta                                                                                                           <  1:703383/140‑1 (MQ=255)

                              gAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGAt                                                                                                                    <  1:1014272/128‑1 (MQ=255)

                                   ttttCTTGTCTGCTGGTGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGGGATCAGGAAGGGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGa                                                                                                   <  2:243608/140‑1 (MQ=255)

                                    tttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGCGGACTGC‑CCCCCCCGGGAGACGATCCTGCCCTATAGATGGAg                                                                                                  >  2:832082/1‑140 (MQ=255)

                                     ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                 <  2:1081400/140‑1 (MQ=255)

                                        ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                              <  2:1382356/140‑1 (MQ=255)

                                        ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                              <  1:939551/140‑1 (MQ=255)

                                        ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                              <  2:153355/140‑1 (MQ=255)

                                         tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTGTAGTTGGAGTGcc                                                                                              >  1:1135005/1‑139 (MQ=255)

                                         tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTGTAGTTGGAGTGcc                                                                                              <  2:1135005/139‑1 (MQ=255)

                                         tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                 <  2:498438/136‑1 (MQ=255)

                                         tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATACTGCCCTATAGTTGGAGTGccc                                                                                             >  2:1091586/1‑140 (MQ=255)

                                          gTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcccc                                                                                            <  1:1257282/140‑1 (MQ=255)

                                            ctgctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCt                                                                                          <  2:652924/140‑1 (MQ=255)

                                               ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTAtt                                                                                       <  2:1533259/140‑1 (MQ=255)

                                                     aataatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTg                                                                                 >  2:1019342/1‑140 (MQ=255)

                                                            aCTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAgg                                                                                   <  1:856097/131‑1 (MQ=255)

                                                                aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                      <  2:422905/140‑1 (MQ=255)

                                                                  tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                      >  2:597083/1‑138 (MQ=255)

                                                                  tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                      <  1:597083/138‑1 (MQ=255)

                                                                  tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                    >  1:1117477/1‑140 (MQ=255)

                                                                    tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCaa                                                                   >  2:1449705/1‑140 (MQ=255)

                                                                    tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCGCTAGAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCaaa                                                                  >  2:1417874/1‑140 (MQ=255)

                                                                     cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                 <  2:667326/140‑1 (MQ=255)

                                                                     cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                 >  1:396111/1‑140 (MQ=255)

                                                                       aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCa                                                               >  2:499141/1‑140 (MQ=255)

                                                                       aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCa                                                               >  1:748635/1‑140 (MQ=255)

                                                                       aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTA‑CGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGACTAAATTTAGCAAATCaa                                                              <  1:1180120/140‑1 (MQ=255)

                                                                        gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCaa                                                              >  2:903318/1‑140 (MQ=255)

                                                                        gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCaa                                                              <  2:679934/140‑1 (MQ=255)

                                                                          tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                            <  1:1029548/140‑1 (MQ=255)

                                                                          tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                            <  2:1394951/140‑1 (MQ=255)

                                                                           gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                      <  1:453871/129‑1 (MQ=255)

                                                                            ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                          <  2:1024000/140‑1 (MQ=255)

                                                                            ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                          >  1:775614/1‑140 (MQ=255)

                                                                            ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGCACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                          >  1:971754/1‑140 (MQ=255)

                                                                                 tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                     <  2:775614/140‑1 (MQ=255)

                                                                                 tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                     <  2:1539145/140‑1 (MQ=255)

                                                                                  ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                            <  2:125968/132‑1 (MQ=255)

                                                                                  ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                            >  1:125968/1‑132 (MQ=255)

                                                                                   gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                    <  1:1449705/140‑1 (MQ=255)

                                                                                    tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                  >  2:420893/1‑140 (MQ=255)

                                                                                    tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                  <  1:830371/140‑1 (MQ=255)

                                                                                    tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                  <  1:146844/140‑1 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                               <  2:379123/140‑1 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                               <  1:97325/140‑1 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                               <  2:1462570/140‑1 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                               <  2:703681/140‑1 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                               <  1:1175258/140‑1 (MQ=255)

                                                                                         tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                             <  1:903318/140‑1 (MQ=255)

                                                                                         tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                             <  1:1091586/140‑1 (MQ=255)

                                                                                          cAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATGCACTTCAGGGGGGtat                                            <  1:832082/140‑1 (MQ=255)

                                                                                              aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTattatt                                        >  1:479935/1‑140 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                   >  1:1176176/1‑140 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                   <  1:499141/140‑1 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGCGGGTATTATTGTaga                                   >  1:1321703/1‑140 (MQ=255)

                                                                                                     tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                             <  1:1205089/128‑1 (MQ=255)

                                                                                                     tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                             >  2:1205089/1‑128 (MQ=255)

                                                                                                     tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTag                                    >  1:1303511/1‑137 (MQ=255)

                                                                                                     tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTag                                    <  2:1303511/137‑1 (MQ=255)

                                                                                                              gATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                        <  2:396111/140‑1 (MQ=255)

                                                                                                              gATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                        <  2:748635/140‑1 (MQ=255)

                                                                                                               aTGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                             >  2:576632/1‑134 (MQ=255)

                                                                                                               aTGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                             <  1:576632/134‑1 (MQ=255)

                                                                                                                tGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATg                      <  1:986837/140‑1 (MQ=255)

                                                                                                                tGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGACCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGAAGAGTTTCCCCATATg                      >  1:34238/1‑140 (MQ=255)

                                                                                                                     atcatcCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCt                 <  2:1117477/140‑1 (MQ=255)

                                                                                                                       catcCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt               >  2:1548200/1‑140 (MQ=255)

                                                                                                                          cccccATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGATTATTGTAGAGTTTCCCCATATGTTTCTATggg            >  1:1316922/1‑140 (MQ=255)

                                                                                                                             ccATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGTGTATTATTGTAGAGTTTCCCCATATGTTTCTAt               <  2:1002893/134‑1 (MQ=255)

                                                                                                                             ccATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGTGTATTATTGTAGAGTTTCCCCATATGTTTCTAt               >  1:1002893/1‑134 (MQ=255)

                                                                                                                                   tgtgGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaa   <  2:1316922/140‑1 (MQ=255)

                                                                                                                                    gtgGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGCGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa  <  2:1321703/140‑1 (MQ=255)

TGGGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAA  >  NC_000913/2765648‑2765918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`