Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,054	(C)7→8	coding (1279/2526 nt)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,047	1	.	C	100.0%	162.0 / NA	46	A429G (GCG→GGG)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/20);  total (26/20)

TGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGT  >  NC_000913/307919‑308181                                                                                                                                  |                                                                                                                                       tgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑ccccccccaag                                                                                                                             >  1:760559/1‑136 (MQ=255)                 ttCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACgg                                                                                                              >  1:1308145/1‑140 (MQ=255)                 ttCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACgg                                                                                                              <  2:746294/140‑1 (MQ=255)                    cTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGTCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGtt                                                                                                           <  2:923503/140‑1 (MQ=255)                       aTTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTca                                                                                                        <  2:1559294/140‑1 (MQ=255)                          aaCCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTcaccc                                                                                                     <  2:1339300/140‑1 (MQ=255)                              cacaGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCg                                                                                                 >  1:934678/1‑140 (MQ=255)                                 aGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCt                                                                                              >  1:750234/1‑140 (MQ=255)                                 aGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCt                                                                                              >  1:823850/1‑140 (MQ=255)                                  gCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTGACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                                                             <  2:760559/140‑1 (MQ=255)                                     aaCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGAtt                                                                                          >  1:1573495/1‑140 (MQ=255)                                      aCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTcaccca                                                                                                    <  2:1289703/129‑1 (MQ=255)                                      aCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTcaccca                                                                                                    >  1:1289703/1‑129 (MQ=255)                                      aCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTa                                                                                         >  1:254703/1‑140 (MQ=255)                                        tAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATc                                                                                       <  2:823850/140‑1 (MQ=255)                                        tAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATc                                                                                       <  2:934678/140‑1 (MQ=255)                                         aaaGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCg                                                                                      <  2:1432248/140‑1 (MQ=255)                                         aaaGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCg                                                                                      <  2:1293508/140‑1 (MQ=255)                                         aaaGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCg                                                                                      <  2:363175/140‑1 (MQ=255)                                             ccTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATc                                                                                  >  2:1272065/1‑140 (MQ=255)                                               tCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGa                                                                                            >  1:642971/1‑128 (MQ=255)                                               tCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGa                                                                                            <  2:642971/128‑1 (MQ=255)                                                 ccGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCata                                                                              >  1:238493/1‑140 (MQ=255)                                                  cGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCatat                                                                             >  1:1506959/1‑140 (MQ=255)                                                          tGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgc                                                                     >  2:197108/1‑140 (MQ=255)                                                          tGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgc                                                                     >  1:268009/1‑140 (MQ=255)                                                             cgcTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccgc                                                                  >  1:1167937/1‑140 (MQ=255)                                                                  aTGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCGTATCCCGTTGCCGCCCAGc                                                             >  1:650362/1‑140 (MQ=255)                                                                  aTGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGc                                                             <  2:1573495/140‑1 (MQ=255)                                                                  aTGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGc                                                             <  2:1308145/140‑1 (MQ=255)                                                                         cGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGt                                                      <  2:268009/140‑1 (MQ=255)                                                                             gctgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTc                                                  >  1:1345648/1‑140 (MQ=255)                                                                                  tCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGa                                             >  2:1263640/1‑140 (MQ=255)                                                                                    cATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGc                                           >  2:1500641/1‑140 (MQ=255)                                                                                      tGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCg                                         >  1:1519519/1‑140 (MQ=255)                                                                                              cACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGa                                 >  1:1418451/1‑140 (MQ=255)                                                                                                      aGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTg                         <  2:1418451/140‑1 (MQ=255)                                                                                                      aGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTg                         <  2:750234/140‑1 (MQ=255)                                                                                                       gCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGc                        >  2:877447/1‑140 (MQ=255)                                                                                                              ttGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCaa                 <  2:1196073/140‑1 (MQ=255)                                                                                                              ttGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCaa                 <  1:1263640/140‑1 (MQ=255)                                                                                                                  aGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAActct             >  1:205724/1‑140 (MQ=255)                                                                                                                       aaCGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTa        >  1:538011/1‑140 (MQ=255)                                                                                                                       aaCGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTa        >  1:710089/1‑140 (MQ=255)                                                                                                                       aaCGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTa        >  2:1523398/1‑140 (MQ=255)                                                                                                                         cGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGc      >  1:1047183/1‑140 (MQ=255)                                                                                                                             gATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGt  <  2:710089/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       TGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGT  >  NC_000913/307919‑308181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑