Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,796,188	(C)5→6	coding (210/960 nt)	waaC →	ADP‑heptose:LPS heptosyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,796,183	1	.	C	100.0%	233.1 / NA	65	A69P (GCC→CCC)	waaC	ADP‑heptose:LPS heptosyltransferase 1
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (33/32);  total (33/32)

TGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑CCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATGGCGTAAAGC  >  NC_000913/3796044‑3796312                                                                                                                                             |                                                                                                                                  tGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCgg                                                                                                                                    <  2:518141/140‑1 (MQ=255)   aTGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑cc                                                                                                                                 <  1:1139605/140‑1 (MQ=255)     gCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑cccc                                                                                                                               >  1:564030/1‑140 (MQ=255)     gCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑cccc                                                                                                                               >  1:1183145/1‑140 (MQ=255)     gCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑cccc                                                                                                                               >  1:1251327/1‑140 (MQ=255)     gCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑cccc                                                                                                                               >  1:251054/1‑140 (MQ=255)       ccAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑cccccc                                                                                                                             >  2:590203/1‑139 (MQ=255)         agcagGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑ccccccat                                                                                                                           >  2:998901/1‑137 (MQ=255)         agcagGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑ccc                                                                                                                                >  2:1318601/1‑135 (MQ=255)         agcagGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑ccc                                                                                                                                <  1:1318601/135‑1 (MQ=255)              gCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAg                                                                                                                       >  1:155495/1‑140 (MQ=255)                aaTCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCg                                                                                                                     >  1:1342259/1‑140 (MQ=255)                 aTCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCgg                                                                                                                    <  1:531931/140‑1 (MQ=255)                     cAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACg                                                                                                                >  2:488885/1‑140 (MQ=255)                      aGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑ccc                                                                                                                                <  1:1296128/122‑1 (MQ=255)                      aGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑ccc                                                                                                                                >  2:1296128/1‑122 (MQ=255)                              aGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGtt                                                                                                       >  1:1505066/1‑140 (MQ=255)                                  tGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGt                                                                                                   <  2:1075396/140‑1 (MQ=255)                                  tGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGt                                                                                                   <  1:1122282/140‑1 (MQ=255)                                   gACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAATCGGAACGCAAAGCGTTTCGTg                                                                                                  >  2:1461280/1‑140 (MQ=255)                                   gACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTg                                                                                                  <  1:1038928/140‑1 (MQ=255)                                    aCTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGa                                                                                                 >  2:234448/1‑140 (MQ=255)                                     cTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGaa                                                                                                <  2:564030/140‑1 (MQ=255)                                     cTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGaa                                                                                                <  2:251054/140‑1 (MQ=255)                                     cTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGaa                                                                                                <  1:580034/140‑1 (MQ=255)                                     cTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGaa                                                                                                <  1:590203/140‑1 (MQ=255)                                        ggtggtGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGGTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAgcg                                                                                             >  2:950397/1‑140 (MQ=255)                                          tggtggAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCt                                                                                           <  2:155495/140‑1 (MQ=255)                                            gtggAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTAc                                                                                         <  2:1183145/140‑1 (MQ=255)                                              ggAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGaa                                                                                                <  1:55132/131‑1 (MQ=255)                                              ggAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGaa                                                                                                >  2:55132/1‑131 (MQ=255)                                                 agaagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCa                                                                                    <  2:402859/140‑1 (MQ=255)                                                 agaagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCa                                                                                    <  1:308802/140‑1 (MQ=255)                                                  gaagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGt                                                                                                   >  1:742716/1‑124 (MQ=255)                                                  gaagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGt                                                                                                   <  2:742716/124‑1 (MQ=255)                                                  gaagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAg                                                                                      >  1:470043/1‑137 (MQ=255)                                                  gaagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAg                                                                                      <  2:470043/137‑1 (MQ=255)                                                   aagGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCaga                                                                                  <  2:607686/140‑1 (MQ=255)                                                    agGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCagag                                                                                 <  2:553729/140‑1 (MQ=255)                                                    agGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCagag                                                                                 >  1:553729/1‑140 (MQ=255)                                                    agGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCagag                                                                                 <  2:17624/140‑1 (MQ=255)                                                      ggTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGaa                                                                               <  1:488885/140‑1 (MQ=255)                                                       gTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAAc                                                                              >  2:367941/1‑140 (MQ=255)                                                       gTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAAc                                                                              >  2:1134899/1‑140 (MQ=255)                                                         tCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACa                                                                                        <  1:534354/128‑1 (MQ=255)                                                         tCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACa                                                                                        >  2:534354/1‑128 (MQ=255)                                                             acaGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAg                                                                                      <  1:817976/126‑1 (MQ=255)                                                             acaGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAg                                                                                      >  2:817976/1‑126 (MQ=255)                                                              caGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACg                                                                       <  2:312673/140‑1 (MQ=255)                                                                gATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCa                                                                     >  2:1339001/1‑140 (MQ=255)                                                                 aTTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAg                                                                    >  2:227547/1‑140 (MQ=255)                                                                 aTTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAg                                                                    >  1:792831/1‑140 (MQ=255)                                                                   tccttcctGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGtt                                                                  <  2:391910/140‑1 (MQ=255)                                                                       tcctGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCg                                                              <  2:1505066/140‑1 (MQ=255)                                                                        cctGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGa                                                             <  2:1251327/140‑1 (MQ=255)                                                                        cctGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGa                                                             <  1:998901/140‑1 (MQ=255)                                                                         ctGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGAc                                                            >  2:1010974/1‑140 (MQ=255)                                                                           ggCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGc                                                          >  2:606368/1‑140 (MQ=255)                                                                           ggCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGc                                                          >  2:1237858/1‑140 (MQ=255)                                                                            gCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCt                                                         <  2:376280/140‑1 (MQ=255)                                                                            gCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCt                                                         <  1:367941/140‑1 (MQ=255)                                                                                  tGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAgggg                                                   >  1:206624/1‑140 (MQ=255)                                                                                  tGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAgggg                                                   <  1:1339001/140‑1 (MQ=255)                                                                                            cGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTaaaaa                                         >  1:842727/1‑140 (MQ=255)                                                                                                 tATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGcgcg                                    >  2:245557/1‑140 (MQ=255)                                                                                                    tCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCgcggcg                                 >  2:391379/1‑140 (MQ=255)                                                                                                         tgGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGt                            >  2:668230/1‑140 (MQ=255)                                                                                                         tgGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGt                            >  2:95117/1‑140 (MQ=255)                                                                                                          ggCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTg                           >  2:1114430/1‑140 (MQ=255)                                                                                                           gCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGa                          <  2:849300/140‑1 (MQ=255)                                                                                                             aaTACGTCGCTGGCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGAcg                        >  2:802337/1‑140 (MQ=255)                                                                                                                         gCGTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCAt            >  1:309826/1‑140 (MQ=255)                                                                                                                          cGTAAAGCCTGGTTTTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATg           >  2:735918/1‑140 (MQ=255)                                                                                                                           gTAAAGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATgg          >  1:103158/1‑140 (MQ=255)                                                                                                                               aGCCTGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATGGCGTa      <  2:309826/140‑1 (MQ=255)                                                                                                                                   tGGTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATGGCGTAAAgc  >  2:605521/1‑140 (MQ=255)                                                                                                                                     gTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATGGCGTaaa    >  1:1071800/1‑136 (MQ=255)                                                                                                                                     gTTCTCGGCCCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATGGCGTaaa    <  2:1071800/136‑1 (MQ=255)                                                                                                                                             |                                                                                                                                  TGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAAAGCCTGGTTCTCGG‑CCCCCATAAAAGCGGAACGCAAAGCGTTTCGTGAAGCGCTACAAGCAGAGAACTATGACGCAGTTATCGACGCTCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGCGCATGGCGTAAAGC  >  NC_000913/3796044‑3796312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑