Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,955,324	(G)6→7	coding (1847/1851 nt)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,955,318	1	.	G	100.0%	184.1 / NA	52	L614R (CTG→CGG)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/26);  total (26/26)

TCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑GGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAAC  >  NC_000913/3955181‑3955448                                                                                                                                           |                                                                                                                                   tcgtGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCAACAAAGGCGCGGTGCGCGATAAATCGAAACT‑gg                                                                                                                                  <  1:12893/140‑1 (MQ=255)  cgtGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑ggg                                                                                                                                 <  2:15211/140‑1 (MQ=255)   gtGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑gggg                                                                                                                                <  1:1071280/140‑1 (MQ=255)   gtGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑gggg                                                                                                                                <  2:1100269/140‑1 (MQ=255)      aaGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGTCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑ggggggg                                                                                                                             <  2:924413/140‑2 (MQ=255)      aaGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑ggggggg                                                                                                                             <  2:1253024/140‑2 (MQ=255)                cGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATgg                                                                                                                    >  1:196008/1‑140 (MQ=255)                 gCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑g                                                                                                                                   >  1:929452/1‑123 (MQ=255)                 gCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑g                                                                                                                                   <  2:929452/123‑1 (MQ=255)                    cGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGa                                                                                                                <  2:28331/140‑1 (MQ=255)                         tGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGc                                                                                                           <  2:776137/140‑1 (MQ=255)                         tGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGc                                                                                                           <  2:820806/140‑1 (MQ=255)                         tGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGc                                                                                                           <  1:306602/140‑1 (MQ=255)                         tGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGc                                                                                                           <  1:1580423/140‑1 (MQ=255)                              aaGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAAccc                                                                                                      <  2:714258/140‑1 (MQ=255)                              aaGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAAccc                                                                                                      <  2:664535/140‑1 (MQ=255)                                 ccTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTg                                                                                                   >  1:307103/1‑140 (MQ=255)                                 ccTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTg                                                                                                   >  1:1068927/1‑140 (MQ=255)                                   tGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTc                                                                                                 <  2:836754/140‑1 (MQ=255)                                   tGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTc                                                                                                 <  1:634462/140‑1 (MQ=255)                                   tGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTc                                                                                                 <  2:1552309/140‑1 (MQ=255)                                    ggACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTcc                                                                                                >  2:99463/1‑140 (MQ=255)                                     gACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCg                                                                                               >  1:670826/1‑140 (MQ=255)                                          cGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCt                                                                                          >  1:1416781/1‑140 (MQ=255)                                          cGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCt                                                                                          >  1:1210654/1‑140 (MQ=255)                                               aaTCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGa                                                                                     <  2:1068927/140‑1 (MQ=255)                                                    tGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTg                                                                                >  2:26962/1‑140 (MQ=255)                                                      aaCGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGcc                                                                              <  2:240793/140‑1 (MQ=255)                                                         gTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGaa                                                                           <  2:383190/140‑1 (MQ=255)                                                              gTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATAttt                                                                      <  2:419023/140‑1 (MQ=255)                                                                    tttGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                                >  1:1090357/1‑140 (MQ=255)                                                                        cccTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATAttt                                                                      >  2:925253/1‑130 (MQ=255)                                                                        cccTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATAttt                                                                      <  1:925253/130‑1 (MQ=255)                                                                        cccTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAA‑GGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTaa                                                                    >  1:508596/1‑131 (MQ=255)                                                                        cccTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAA‑GGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTaa                                                                    <  2:508596/131‑1 (MQ=255)                                                                         ccTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGtgc                                                           >  2:132512/1‑140 (MQ=255)                                                                           tGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGtgctg                                                         <  2:1090357/140‑1 (MQ=255)                                                                             cGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGTTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcg                                                       >  1:694479/1‑140 (MQ=255)                                                                             cGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcg                                                       >  1:458272/1‑140 (MQ=255)                                                                             cGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcg                                                       >  2:789918/1‑140 (MQ=255)                                                                             cGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcg                                                       >  1:540110/1‑140 (MQ=255)                                                                               tGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgcg                                                     <  1:870954/140‑1 (MQ=255)                                                                                gCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgcgc                                                    <  2:650926/140‑1 (MQ=255)                                                                                gCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgcgc                                                    <  2:670826/140‑1 (MQ=255)                                                                                   tATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGcc                                                 >  2:1600086/1‑140 (MQ=255)                                                                                       ccAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGGAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGCGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGtt                                             >  2:568843/1‑140 (MQ=255)                                                                                            cTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGa                                        >  2:167174/1‑140 (MQ=255)                                                                                              ggCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAgg                                      <  1:532246/140‑1 (MQ=255)                                                                                               gCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAggc                                     >  2:127310/1‑140 (MQ=255)                                                                                                   ccAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGgcggcg                                 <  1:1161844/140‑1 (MQ=255)                                                                                                    cAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGgcgc                                <  1:132512/140‑1 (MQ=255)                                                                                                     aGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCa                               >  2:1542221/1‑140 (MQ=255)                                                                                                     aGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCa                               >  2:1029534/1‑140 (MQ=255)                                                                                                       cgcCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAgg                             >  2:1406980/1‑140 (MQ=255)                                                                                                            aCAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcg                        <  1:1600086/140‑1 (MQ=255)                                                                                                                 ggCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCt                   <  1:167174/140‑1 (MQ=255)                                                                                                                      ggTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACaaa              >  2:1218425/1‑140 (MQ=255)                                                                                                                            cgATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgc                       <  1:794228/125‑1 (MQ=255)                                                                                                                            cgATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgc                       >  2:794228/1‑125 (MQ=255)                                                                                                                                  aTCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAAc  >  2:533940/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                   TCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑GGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAAC  >  NC_000913/3955181‑3955448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑