Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,885	C→T	P27S (CCG→TCG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,885	0	C	T	100.0%	331.3 / NA	93	P27S (CCG→TCG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (54/39);  total (54/39)

GATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCG  >  NC_000913/45749‑46019                                                                                                                                         |                                                                                                                                       gATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGt                                                                                                                                     <  1:972460/140‑1 (MQ=255)       ggTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTgg                                                                                                                               <  2:1333283/140‑1 (MQ=255)        gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  2:1507554/140‑1 (MQ=255)        gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  1:816362/140‑1 (MQ=255)        gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  1:530396/140‑1 (MQ=255)        gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  1:238573/140‑1 (MQ=255)        gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  2:1199523/140‑1 (MQ=255)            gATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGt                                                                                                                          <  2:1016912/140‑1 (MQ=255)             aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGtt                                                                                                                         >  1:323370/1‑140 (MQ=255)             aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGtt                                                                                                                         >  1:267439/1‑140 (MQ=255)             aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGtt                                                                                                                         <  1:1487512/140‑1 (MQ=255)             aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGtt                                                                                                                         >  2:809275/1‑140 (MQ=255)                  tAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTa                                                                                                                    <  2:1035449/140‑1 (MQ=255)                  tAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTa                                                                                                                    >  1:398204/1‑140 (MQ=255)                   aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              >  2:740888/1‑129 (MQ=255)                   aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  1:740888/129‑1 (MQ=255)                   aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTAc                                                                                                                   <  1:468907/140‑1 (MQ=255)                   aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTAc                                                                                                                   <  1:755398/140‑1 (MQ=255)                     cgccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              >  1:1311366/1‑127 (MQ=255)                     cgccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  2:1311366/127‑1 (MQ=255)                     cgccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTg                                                                                                                 <  2:1210154/140‑1 (MQ=255)                     cgccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTg                                                                                                                 >  1:274380/1‑140 (MQ=255)                       ccgcACTGACTCTCATTGCAAAAAACAAGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGt                                                                                                               <  1:978715/140‑1 (MQ=255)                                  ctcATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATtggcg                                                                                                    <  1:898320/140‑1 (MQ=255)                                         cAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCt                                                                                             >  2:1210783/1‑140 (MQ=255)                                         cAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCt                                                                                             >  1:955700/1‑140 (MQ=255)                                           aaaaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATAGTTATGTACTGGTAATGATTGGCGTGGCGCTgg                                                                                           >  1:1197150/1‑140 (MQ=255)                                             aaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAg                                                                                         >  1:1258075/1‑140 (MQ=255)                                                        cccATGCAACCGTCCAGAACCTTGGACGATCTCAAGTTCCCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGcc                                                                              <  2:267439/139‑1 (MQ=255)                                                        aCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGcc                                                                              >  1:653120/1‑140 (MQ=255)                                                        aCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGcc                                                                              >  1:857034/1‑140 (MQ=255)                                                             gCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGgcgc                                                                         >  1:801888/1‑140 (MQ=255)                                                               aaCCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTg                                                                       <  2:398204/140‑1 (MQ=255)                                                                  cGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGaaa                                                                    >  2:818917/1‑140 (MQ=255)                                                                   gTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAAc                                                                   >  1:820956/1‑140 (MQ=255)                                                                   gTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAAc                                                                   >  2:22893/1‑140 (MQ=255)                                                                    tCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACt                                                                  >  2:610707/1‑140 (MQ=255)                                                                      cAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTgg                                                                >  2:430983/1‑140 (MQ=255)                                                                        gAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGAc                                                              <  2:857034/140‑1 (MQ=255)                                                                         aaaCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACg                                                             >  2:1558633/1‑140 (MQ=255)                                                                         aaaCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACg                                                             <  2:373073/140‑1 (MQ=255)                                                                         aaaCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACg                                                             <  1:174626/140‑1 (MQ=255)                                                                         aaaCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACg                                                             <  2:820956/140‑1 (MQ=255)                                                                              ttGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGa                                                         >  1:632209/1‑139 (MQ=255)                                                                              ttGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGa                                                         <  2:632209/139‑1 (MQ=255)                                                                               tGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACt                                                       >  1:881191/1‑140 (MQ=255)                                                                                 aCGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTgg                                                     <  1:1558633/140‑1 (MQ=255)                                                                                      ctcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTgg                                                >  1:150061/1‑140 (MQ=255)                                                                                      ctcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTgg                                                >  1:276465/1‑140 (MQ=255)                                                                                      ctcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTgg                                                >  1:1107023/1‑140 (MQ=255)                                                                                       tcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGa                                                         <  1:953626/130‑1 (MQ=255)                                                                                       tcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGa                                                         >  2:953626/1‑130 (MQ=255)                                                                                       tcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGc                                               >  2:418813/1‑140 (MQ=255)                                                                                          aaTTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTg                                            <  1:430983/140‑1 (MQ=255)                                                                                              ctcctcTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTgg                                        >  1:902252/1‑140 (MQ=255)                                                                                               tcctcTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTggg                                       >  1:148055/1‑140 (MQ=255)                                                                                               tcctcTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTggg                                       >  1:1517031/1‑140 (MQ=255)                                                                                                 ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                     >  1:111287/1‑140 (MQ=255)                                                                                                 ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                     >  1:261223/1‑140 (MQ=255)                                                                                                 ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                     >  1:569826/1‑140 (MQ=255)                                                                                                 ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                     >  2:454943/1‑140 (MQ=255)                                                                                                 ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGCGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                     >  2:1573179/1‑140 (MQ=255)                                                                                                   ctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGcgcg                                   >  2:1593999/1‑140 (MQ=255)                                                                                                    tATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCgg                                  >  1:74136/1‑140 (MQ=255)                                                                                                      ttCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCT‑GATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGaa                               >  2:194113/1‑140 (MQ=255)                                                                                                        cACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAAc                              <  2:148055/140‑1 (MQ=255)                                                                                                         aCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACg                             >  1:278840/1‑140 (MQ=255)                                                                                                           cgccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                           >  2:47221/1‑140 (MQ=255)                                                                                                           cgccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                           >  2:99361/1‑140 (MQ=255)                                                                                                           cgccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                           >  2:1083579/1‑140 (MQ=255)                                                                                                           cgccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                           >  2:1056695/1‑140 (MQ=255)                                                                                                           cgccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                           >  1:796874/1‑140 (MQ=255)                                                                                                             ccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCg                         >  2:1602976/1‑140 (MQ=255)                                                                                                              cgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCt                                          >  2:1543961/1‑122 (MQ=255)                                                                                                              cgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCt                                          <  1:1543961/122‑1 (MQ=255)                                                                                                              cgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGc                        >  1:1505408/1‑140 (MQ=255)                                                                                                              cgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGc                        >  1:564808/1‑140 (MQ=255)                                                                                                                cATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCg                      <  2:955700/140‑1 (MQ=255)                                                                                                                cATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCg                      >  1:1203734/1‑140 (MQ=255)                                                                                                                   tttGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGc                   <  2:1287519/140‑1 (MQ=255)                                                                                                                     tGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTg                 <  2:111287/140‑1 (MQ=255)                                                                                                                       cTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGtt               <  1:22893/140‑1 (MQ=255)                                                                                                                          tgGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGt            <  1:610707/140‑1 (MQ=255)                                                                                                                             ggAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTgg         <  2:74136/140‑1 (MQ=255)                                                                                                                               aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       <  1:1056695/140‑1 (MQ=255)                                                                                                                               aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       >  1:231864/1‑140 (MQ=255)                                                                                                                               aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       >  1:762519/1‑140 (MQ=255)                                                                                                                               aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       <  2:1517031/140‑1 (MQ=255)                                                                                                                                  cGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACAt    <  2:323370/140‑1 (MQ=255)                                                                                                                                    gtggtTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       <  1:1368313/135‑1 (MQ=255)                                                                                                                                    gtggtTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       >  2:1368313/1‑135 (MQ=255)                                                                                                                                    gtggtTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg  >  2:549877/1‑140 (MQ=255)                                                                                                                                    gtggtTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg  >  2:129610/1‑140 (MQ=255)                                                                                                                                    gtggtTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg  <  1:1083579/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCG  >  NC_000913/45749‑46019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑