Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,192,296	T→C	K114E (AAA→GAA)	thiG ←	1‑deoxy‑D‑xylulose 5‑phosphate:thiol sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,192,296	0	T	C	100.0%	244.9 / NA	68	K114E (AAA→GAA)	thiG	1‑deoxy‑D‑xylulose 5‑phosphate:thiol sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/27);  total (41/27)

ATTCGAGCCAATCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGCCCCGG  >  NC_000913/4192162‑4192427                                                                                                                                       |                                                                                                                                    aTTCGAGCCAATCAGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAggg                                                                                                                                >  2:1210915/1‑140 (MQ=255)  ttCGAGCCAATCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGt                                                                                                                               <  1:557704/140‑1 (MQ=255)       gCCAATCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGa                                                                                                                          >  1:378371/1‑140 (MQ=255)       gCCAATCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGa                                                                                                                          >  2:1553600/1‑140 (MQ=255)       gCCAATCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGa                                                                                                                          >  1:876561/1‑140 (MQ=255)       gCCAATCGGCGCGACGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAggctgtctc                                                                                                                          >  2:828166/1‑133 (MQ=255)          aaTCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATgg                                                                                                                       <  1:1555980/140‑1 (MQ=255)          aaTCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATgg                                                                                                                       <  2:940480/140‑1 (MQ=255)          aaTCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATgg                                                                                                                       <  1:1549583/140‑1 (MQ=255)           aTCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATggg                                                                                                                      >  1:1209897/1‑140 (MQ=255)              ggCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATc                                                                                                                   <  1:745228/140‑1 (MQ=255)                                  cGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGt                                                                                               <  1:1553600/140‑1 (MQ=255)                                          aGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGa                                                                                       <  2:584631/140‑1 (MQ=255)                                            ccGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAAt                                                                                     >  2:1181104/1‑140 (MQ=255)                                              gACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAAtct                                                                                   <  2:876561/140‑1 (MQ=255)                                               aCTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAAtcac                                                                                  >  2:1090044/1‑138 (MQ=255)                                                   cttcCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAAt                                                                              <  2:198658/140‑1 (MQ=255)                                                         gACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAAc                                                                        <  1:877683/140‑1 (MQ=255)                                                         gACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAAc                                                                        <  2:1209897/140‑1 (MQ=255)                                                         gACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAAc                                                                        <  2:1451156/140‑1 (MQ=255)                                                              ttACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGtt                                                                   >  1:761216/1‑140 (MQ=255)                                                               tACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAAtctc                                                                                  >  2:105147/1‑124 (MQ=255)                                                               tACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAAtctc                                                                                  <  1:105147/124‑1 (MQ=255)                                                               tACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGttt                                                                  <  1:1210915/140‑1 (MQ=255)                                                                      tACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTa                                                           >  2:1147370/1‑140 (MQ=255)                                                                      tACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTa                                                           >  1:1065765/1‑140 (MQ=255)                                                                      tACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTa                                                           >  2:1503037/1‑140 (MQ=255)                                                                       aCCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTaa                                                          <  1:1147370/140‑1 (MQ=255)                                                                         cGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACg                                                        >  1:1351937/1‑140 (MQ=255)                                                                           gATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCt                                                      >  2:430063/1‑140 (MQ=255)                                                                           gATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCt                                                      >  2:385232/1‑140 (MQ=255)                                                                            aTCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGcc                                                             <  1:763415/132‑1 (MQ=255)                                                                            aTCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGcc                                                             <  1:215579/132‑1 (MQ=255)                                                                            aTCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGcc                                                             >  2:763415/1‑132 (MQ=255)                                                                            aTCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGcc                                                             >  2:215579/1‑132 (MQ=255)                                                                            aTCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCtt                                                     >  1:1302735/1‑140 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCATGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACg                                                 >  1:1416817/1‑140 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGttt                                                                  >  1:834714/1‑123 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGttt                                                                  <  2:834714/123‑1 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCAcc                                                 >  1:955573/1‑139 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACg                                                 >  2:842087/1‑140 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACg                                                 >  1:16428/1‑140 (MQ=255)                                                                                gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACg                                                 >  2:1507486/1‑140 (MQ=255)                                                                                           aGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATggg                                      >  1:828948/1‑140 (MQ=255)                                                                                           aGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATggg                                      >  1:568217/1‑140 (MQ=255)                                                                                            ggCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGggc                                     >  1:1595840/1‑140 (MQ=255)                                                                                                 caCGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGa                                >  1:1084562/1‑140 (MQ=255)                                                                                                 caCGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGa                                >  1:720728/1‑140 (MQ=255)                                                                                                      cAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATg                           <  1:842087/140‑1 (MQ=255)                                                                                                                 tgtACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGc                <  1:1181104/140‑1 (MQ=255)                                                                                                                   tACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGaaa                              >  2:339502/1‑124 (MQ=255)                                                                                                                   tACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGaaa                              <  1:339502/124‑1 (MQ=255)                                                                                                                   tACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTg              >  1:1527246/1‑140 (MQ=255)                                                                                                                   tACCAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTg              >  1:402249/1‑140 (MQ=255)                                                                                                                     ccAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTTACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGtt            >  1:541090/1‑140 (MQ=255)                                                                                                                     ccAGCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGtt            >  1:256948/1‑140 (MQ=255)                                                                                                                        gCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTc         >  1:13522/1‑140 (MQ=255)                                                                                                                        gCGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTc         >  2:912798/1‑140 (MQ=255)                                                                                                                         cGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCg        >  2:490042/1‑140 (MQ=255)                                                                                                                         cGTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCg        >  2:147790/1‑140 (MQ=255)                                                                                                                          gTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGc       <  2:1084562/140‑1 (MQ=255)                                                                                                                          gTTTCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGc       <  2:828948/140‑1 (MQ=255)                                                                                                                           tttCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCg                 <  2:1113607/129‑1 (MQ=255)                                                                                                                           tttCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCg                 >  1:1113607/1‑129 (MQ=255)                                                                                                                            ttCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGccc     <  2:1065765/140‑1 (MQ=255)                                                                                                                             tCGGCGGCTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGcccc    >  1:767413/1‑140 (MQ=255)                                                                                                                               ggcggcTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGCCCCgg  <  2:1351937/140‑1 (MQ=255)                                                                                                                               ggcggcTTCCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGCCCCgg  <  1:1507486/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    ATTCGAGCCAATCGGCGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTGTGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAATGGCTTCTTCCGCTGTTTTCGCCCCGG  >  NC_000913/4192162‑4192427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑