Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,247,335	C→T	G184G (GGC→GGT)	malK →	maltose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,247,335	0	C	T	100.0%	283.3 / NA	80	G184G (GGC→GGT)	malK	maltose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/43);  total (37/43)

CGTCAGCGTGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGCCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTATCCGGCAGACCGTTTT  >  NC_000913/4247198‑4247470                                                                                                                                          |                                                                                                                                        cgtcagcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCg                                                                                                                                       >  2:1523134/1‑140 (MQ=255) cgtcagcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCg                                                                                                                                       >  2:664756/1‑140 (MQ=255)   tcagcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGca                                                                                                                                     <  1:1393385/140‑1 (MQ=255)     agcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGcaca                                                                                                                                   <  1:664756/140‑1 (MQ=255)      gcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGcac                                                                                                                                    <  1:231844/138‑1 (MQ=255)      gcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGcac                                                                                                                                    >  2:231844/1‑138 (MQ=255)      gcgtGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCAT‑AACGCCTGGGTCGCACAAt                                                                                                                                 >  1:847515/1‑140 (MQ=255)          gtgGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGAt                                                                                                                              <  1:924237/140‑1 (MQ=255)          gtgGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGAt                                                                                                                              <  1:886154/140‑1 (MQ=255)              cGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTAc                                                                                                                          <  2:1503848/140‑1 (MQ=255)               gATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACg                                                                                                                         <  1:1234681/140‑1 (MQ=255)                aTTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGt                                                                                                                        <  1:649677/140‑1 (MQ=255)                 ttGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTc                                                                                                                       >  1:1333625/1‑140 (MQ=255)                 ttGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTc                                                                                                                       >  1:1556629/1‑140 (MQ=255)                    gCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCAcc                                                                                                                    <  2:280252/140‑1 (MQ=255)                    gCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCAcc                                                                                                                    <  1:264852/140‑1 (MQ=255)                    gCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCAcc                                                                                                                    <  1:219046/140‑1 (MQ=255)                         aCGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGa                                                                                                               <  2:1556629/140‑1 (MQ=255)                               gtggCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGt                                                                                                         <  2:730337/140‑1 (MQ=255)                                  gCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGa                                                                                                      >  2:1216488/1‑140 (MQ=255)                                   ccGAGCCAGGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGaa                                                                                                     >  2:711852/1‑140 (MQ=255)                                     gAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGc                                                                                                   >  2:768506/1‑140 (MQ=255)                                     gAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGc                                                                                                   >  1:1286676/1‑140 (MQ=255)                                      aGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCg                                                                                                  >  1:1107011/1‑140 (MQ=255)                                            gCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACg                                                                                            <  2:174035/140‑1 (MQ=255)                                                  ttttGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGcc                                                                                      >  1:376/1‑140 (MQ=255)                                                     tGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGAc                                                                                   >  1:1046195/1‑140 (MQ=255)                                                      gCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACa                                                                                  <  1:1523134/140‑1 (MQ=255)                                                       cTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACaa                                                                                 >  2:176037/1‑140 (MQ=255)                                                           aTGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATc                                                                             >  2:1035562/1‑140 (MQ=255)                                                           aTGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGA‑AAAATCg                                                                            <  2:1252119/140‑1 (MQ=255)                                                              aaCCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCgtg                                                                          <  1:1556155/140‑1 (MQ=255)                                                                  gCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGc                                                                      <  2:1046195/140‑1 (MQ=255)                                                                      tcCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTg                                                                    >  2:1424131/1‑138 (MQ=255)                                                                      tcCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTg                                                                    <  1:1424131/138‑1 (MQ=255)                                                                           ccTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCg                                                             >  2:563499/1‑140 (MQ=255)                                                                              cGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTc                                                          >  1:1044335/1‑140 (MQ=255)                                                                                aTGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTcgc                                                        >  2:489333/1‑140 (MQ=255)                                                                                aTGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTcgc                                                        <  2:1071441/140‑1 (MQ=255)                                                                                      cACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  <  2:1044335/140‑1 (MQ=255)                                                                                      cACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  <  2:1107011/140‑1 (MQ=255)                                                                                      cACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  >  2:369963/1‑140 (MQ=255)                                                                                      cACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  <  1:369963/140‑1 (MQ=255)                                                                                       aCTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcgc                                                 <  2:1333625/140‑1 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  >  2:601559/1‑135 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  <  2:570114/135‑1 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  <  1:601559/135‑1 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGgcg                                                  >  1:570114/1‑135 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGt                                             >  2:295279/1‑140 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGt                                             >  1:1192292/1‑140 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGt                                             >  1:1383141/1‑140 (MQ=255)                                                                                           cGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGt                                             >  1:871684/1‑140 (MQ=255)                                                                                             tgtgCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTg                                           >  1:164752/1‑140 (MQ=255)                                                                                             tgtgCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATAAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTg                                           >  2:693978/1‑140 (MQ=255)                                                                                                gCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGa                                        <  2:376/140‑1 (MQ=255)                                                                                                     tGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCg                                   >  2:963797/1‑140 (MQ=255)                                                                                                     tGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCg                                   >  2:367601/1‑140 (MQ=255)                                                                                                        gTATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGGCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTg                                <  2:530522/140‑1 (MQ=255)                                                                                                         tATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTgg                               <  2:164752/140‑1 (MQ=255)                                                                                                         tATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTgg                               >  1:1093571/1‑140 (MQ=255)                                                                                                         tATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTgg                               >  2:239706/1‑140 (MQ=255)                                                                                                         tATCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTgg                               <  2:850917/140‑1 (MQ=255)                                                                                                           tCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCATAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAg                             <  1:411006/140‑1 (MQ=255)                                                                                                           tCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAg                             <  2:75186/140‑1 (MQ=255)                                                                                                           tCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAg                             <  2:1161661/140‑1 (MQ=255)                                                                                                           tCGAAATCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAg                             <  1:1216488/140‑1 (MQ=255)                                                                                                              aaaTCTCCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTg                          <  2:871684/140‑1 (MQ=255)                                                                                                                aTCTCCCGTCTGCATAAACGCCTGGGTAGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGa                              <  2:93061/134‑1 (MQ=255)                                                                                                                aTCTCCCGTCTGCATAAACGCCTGGGTAGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGa                              >  1:93061/1‑134 (MQ=255)                                                                                                                aTCTCCCGTCTGCAT‑AACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTAc                       <  2:847515/140‑1 (MQ=255)                                                                                                                 tctcCCGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTAc                       <  1:176037/140‑1 (MQ=255)                                                                                                                    cccGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCAc                    <  1:1148237/140‑1 (MQ=255)                                                                                                                     ccGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACt                   >  1:346532/1‑140 (MQ=255)                                                                                                                     ccGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACt                   <  1:489333/140‑1 (MQ=255)                                                                                                                      cGTCTGCATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTa                  <  2:1286676/140‑1 (MQ=255)                                                                                                                            cATAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGATGGGAAACCGCTGGAGCTGTACCACTATCCGGc            >  2:1278406/1‑140 (MQ=255)                                                                                                                             aTAAACGCCTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTATCCGGCa           <  1:1543964/140‑1 (MQ=255)                                                                                                                                    ccTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTATCCGGCAGa         <  2:968383/135‑1 (MQ=255)                                                                                                                                    ccTGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTATCCGGCAGa         >  1:968383/1‑135 (MQ=255)                                                                                                                                      tGGGTCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTATCCGGCAGACCGtttt  >  2:746231/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGTCAGCGTGTGGCGATTGGCCGTACGCTGGTGGCCGAGCCAAGCGTATTTTTGCTCGATGAACCGCTCTCCAACCTCGATGCTGCACTGCGTGTGCAAATGCGTATCGAAATCTCCCGTCTGCATAAACGCCTGGGCCGCACAATGATTTACGTCACCCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCGTGGCGCAGGTTGGGAAACCGCTGGAGCTGTACCACTATCCGGCAGACCGTTTT  >  NC_000913/4247198‑4247470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑