Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,280,799	A→G	T274A (ACC→GCC)	yjcE →	putative transporter YjcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,280,799	0	A	G	100.0%	270.2 / NA	76	T274A (ACC→GCC)	yjcE	putative transporter YjcE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/40);  total (36/40)

TGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGT  >  NC_000913/4280662‑4280928                                                                                                                                          |                                                                                                                                  tgcCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGcc                                                                                                                                 >  1:809490/1‑140 (MQ=255) tgcCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGcc                                                                                                                                 >  1:1580800/1‑140 (MQ=255)  gcCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCt                                                                                                                                >  1:805006/1‑140 (MQ=255)    cATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTgg                                                                                                                              >  2:254276/1‑140 (MQ=255)     aTTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTggg                                                                                                                             <  1:587519/140‑1 (MQ=255)             cttATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCta                                                                                                                     >  2:817284/1‑139 (MQ=255)             cttATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTg                                                                                                                     >  2:646405/1‑140 (MQ=255)                  cTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCACTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAAtt                                                                                                                <  1:1401291/140‑1 (MQ=255)                   tGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAAttt                                                                                                               >  1:1351811/1‑140 (MQ=255)                     aTTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGt                                                                                                             >  2:281011/1‑140 (MQ=255)                        gCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCtt                                                                                                          >  1:1462128/1‑140 (MQ=255)                         ccGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCttt                                                                                                         >  1:446861/1‑140 (MQ=255)                          cGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTa                                                                                                        <  2:1580800/140‑1 (MQ=255)                           gAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTaa                                                                                                       <  2:1511134/140‑1 (MQ=255)                            aaCATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAAc                                                                                                      >  1:1394940/1‑140 (MQ=255)                              cATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCGCCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACgg                                                                                                    <  1:592263/140‑1 (MQ=255)                                tatTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCa                                                                                                  <  2:120451/140‑1 (MQ=255)                                      cGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGgtgt                                                                                            >  1:1325299/1‑140 (MQ=255)                                        tttCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTc                                                                                          >  1:1441942/1‑140 (MQ=255)                                        tttCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTc                                                                                          <  1:736296/140‑1 (MQ=255)                                         ttCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATgg                                                                                               >  2:729170/1‑134 (MQ=255)                                         ttCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATgg                                                                                               <  1:729170/134‑1 (MQ=255)                                         ttCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTcc                                                                                         <  2:1462128/140‑1 (MQ=255)                                          tctggCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCct                                                                                        >  2:1358683/4‑140 (MQ=255)                                           ccGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCctg                                                                                       <  2:216896/140‑1 (MQ=255)                                               cATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGt                                                                                   <  1:1540066/140‑1 (MQ=255)                                               cATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGt                                                                                   <  2:1394940/140‑1 (MQ=255)                                               cATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTAGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGt                                                                                   <  1:817284/140‑1 (MQ=255)                                                aTCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGtt                                                                                  <  1:1409812/140‑1 (MQ=255)                                                            gTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCt                                                                      <  1:1358683/140‑1 (MQ=255)                                                            gTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCt                                                                      <  1:281011/140‑1 (MQ=255)                                                               gccgccGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGcc                                                                   <  1:1163446/140‑1 (MQ=255)                                                               gccgccGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGcc                                                                   >  2:1364644/1‑140 (MQ=255)                                                               gccgccGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGcc                                                                   >  2:1226679/1‑140 (MQ=255)                                                               gccgccGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGcc                                                                   <  2:1325299/140‑1 (MQ=255)                                                                 cgccgGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCgg                                                                 <  2:1441942/140‑1 (MQ=255)                                                                           aCCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGga                                                       >  1:675152/1‑140 (MQ=255)                                                                           aCCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGga                                                       >  2:139157/1‑140 (MQ=255)                                                                            ccATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGgag                                                      <  1:1226679/140‑1 (MQ=255)                                                                            ccATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGgag                                                      <  2:805006/140‑1 (MQ=255)                                                                                    cGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCt                                              <  1:658445/140‑1 (MQ=255)                                                                                      cTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGa                                            >  2:415920/1‑140 (MQ=255)                                                                                      cTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGa                                            >  2:431283/1‑140 (MQ=255)                                                                                      cTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGa                                            >  2:616806/1‑140 (MQ=255)                                                                                          ggTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgc                                        >  1:1302896/1‑140 (MQ=255)                                                                                          ggTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgc                                        >  2:1197264/1‑140 (MQ=255)                                                                                          ggTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgc                                        >  1:375822/1‑140 (MQ=255)                                                                                           gtgtGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgca                                       >  1:528005/1‑140 (MQ=255)                                                                                             gtgATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgcagc                                     >  2:245845/1‑140 (MQ=255)                                                                                             gtgATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGAATTCTGGAGACGTCGCTGATGgcagc                                     >  1:1322841/1‑140 (MQ=255)                                                                                                 tgcgccgtgcgccgCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgcag                                      <  2:1166018/135‑1 (MQ=255)                                                                                                 tgcgccgtgcgccgCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgcag                                      >  1:1166018/1‑135 (MQ=255)                                                                                                 tgcgccgtgcgccgCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGaa                                 >  1:346303/1‑140 (MQ=255)                                                                                                   cgccgtgcgccgCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAAt                               >  1:1095983/1‑140 (MQ=255)                                                                                                     ccgtgcgccgCTGGCAATCCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCg                             >  1:1062063/1‑140 (MQ=255)                                                                                                        tgcgccgCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGAt                                           <  1:489829/123‑1 (MQ=255)                                                                                                        tgcgccgCTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGAt                                           >  2:489829/1‑123 (MQ=255)                                                                                                          cgccgcTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCt                        >  1:167520/1‑140 (MQ=255)                                                                                                           gccgcTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTa                       <  2:1302896/140‑1 (MQ=255)                                                                                                           gccgcTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTa                       <  1:1197264/140‑1 (MQ=255)                                                                                                               cTGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGt                   <  1:646405/140‑1 (MQ=255)                                                                                                                tGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgcagca                                    >  1:1280581/1‑122 (MQ=255)                                                                                                                tGGCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGgcagca                                    <  2:1280581/122‑1 (MQ=255)                                                                                                                  gCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGa                <  1:1364644/140‑1 (MQ=255)                                                                                                                  gCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGa                <  1:431283/140‑1 (MQ=255)                                                                                                                  gCAATGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGa                <  2:1351811/140‑1 (MQ=255)                                                                                                                     aTGCGCCTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAAt             <  1:139157/140‑1 (MQ=255)                                                                                                                          ccTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGa        <  1:415920/140‑1 (MQ=255)                                                                                                                          ccTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGa        <  1:254276/140‑1 (MQ=255)                                                                                                                          ccTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGa        <  2:446861/140‑1 (MQ=255)                                                                                                                          ccTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGa        <  2:346303/140‑1 (MQ=255)                                                                                                                          ccTGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGa        <  1:938084/140‑1 (MQ=255)                                                                                                                            tGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATg      <  2:528005/140‑1 (MQ=255)                                                                                                                            tGCGTGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATg      <  2:1475437/140‑1 (MQ=255)                                                                                                                                tGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGt  >  2:537215/1‑140 (MQ=255)                                                                                                                                tGCAAACAGCGCCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGt  <  2:675152/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  TGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTGGGCGATGCTGGAATTTGTCTTTAACGGCATGGTGTTCCTGCTGTTAGGTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGT  >  NC_000913/4280662‑4280928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑