BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,377	T→C	intergenic (‑51/+27)	fumB ← / ← dcuB	fumarase B/anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,377	0	T	C	100.0%	447.4 / NA	122	intergenic (‑51/+27)	fumB/dcuB	fumarase B/anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (70/52); total (70/52)

Only 100 of 123 total aligned reads displayed.

GTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAC  >  NC_000913/4347243‑4347507

gTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCa                                                                                                                               <  2:17222/140‑1 (MQ=255)

 tAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCAc                                                                                                                              <  1:1293971/140‑1 (MQ=255)

   aTCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACgg                                                                                                                            >  1:328957/1‑140 (MQ=255)

   aTCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACgg                                                                                                                            >  2:1393283/1‑140 (MQ=255)

    tCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACggg                                                                                                                           >  2:823824/1‑140 (MQ=255)

       gAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGggcgg                                                                                                                        <  1:793168/140‑1 (MQ=255)

         aGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCa                                                                                                                      >  1:243037/1‑140 (MQ=255)

             aGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCa                                                                                                                  >  1:161864/1‑140 (MQ=255)

               tAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCaaa                                                                                                                <  1:498846/140‑1 (MQ=255)

               tAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCaaa                                                                                                                <  2:442660/140‑1 (MQ=255)

               tAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCaaa                                                                                                                >  1:1392844/1‑140 (MQ=255)

                 gATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGt                                                                                                              <  2:1268953/140‑1 (MQ=255)

                 gATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGt                                                                                                              <  2:243037/140‑1 (MQ=255)

                  aTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTg                                                                                                             <  1:430919/140‑1 (MQ=255)

                     gTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGggc                                                                                                                          <  2:944818/124‑1 (MQ=255)

                     gTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGggc                                                                                                                          >  1:944818/1‑124 (MQ=255)

                     gTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCAt                                                                                                          <  1:823824/140‑1 (MQ=255)

                       aTTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCAttt                                                                                                        >  2:1414686/1‑140 (MQ=255)

                       aTTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCAttt                                                                                                        >  2:542636/1‑140 (MQ=255)

                       aTTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCAttt                                                                                                        >  1:994770/1‑140 (MQ=255)

                       aTTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCAttt                                                                                                        >  1:304353/1‑140 (MQ=255)

                        ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCa                                                                                                                      <  2:1040526/125‑1 (MQ=255)

                        ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCa                                                                                                                      >  1:1040526/1‑125 (MQ=255)

                        ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTta                                                                                                       >  2:1305288/1‑140 (MQ=255)

                        ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTta                                                                                                       >  2:1607/1‑140 (MQ=255)

                           ggTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATaa                                                                                                    >  2:641908/1‑140 (MQ=255)

                           ggTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATaa                                                                                                    >  2:691594/1‑140 (MQ=255)

                            gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   <  1:452600/140‑1 (MQ=255)

                            gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   >  2:452600/1‑140 (MQ=255)

                            gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   <  2:1404101/140‑1 (MQ=255)

                            gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   <  1:691594/140‑1 (MQ=255)

                            gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   >  1:204744/1‑140 (MQ=255)

                            gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   >  2:1101028/1‑140 (MQ=255)

                             tATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTtata                                                                                                     <  1:277891/137‑1 (MQ=255)

                             tATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTtata                                                                                                     >  2:277891/1‑137 (MQ=255)

                             tATTGTCCTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGa                                                                                                  >  1:1262611/1‑140 (MQ=255)

                                tGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAAcc                                                                                               >  1:306620/1‑140 (MQ=255)

                                  tCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGTCACGCAAAGTGCATTTATAAGAAc                                                                                                >  2:262962/1‑137 (MQ=255)

                                  tCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGTCACGCAAAGTGCATTTATAAGAAc                                                                                                <  1:262962/137‑1 (MQ=255)

                                       ccccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   <  1:206167/129‑1 (MQ=255)

                                       ccccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACAt                                                                                        >  2:1181220/1‑140 (MQ=255)

                                       ccccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACAt                                                                                        >  2:331251/1‑140 (MQ=255)

                                         ccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATcg                                                                                      <  1:1063070/140‑1 (MQ=255)

                                            tCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   >  2:1390841/1‑124 (MQ=255)

                                            tCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   <  1:1027534/124‑1 (MQ=255)

                                            tCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAg                                                                                                   <  1:1390841/124‑1 (MQ=255)

                                                 aaaGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAg                                                                              >  1:7125/1‑140 (MQ=255)

                                                       gCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAg                                                                        <  1:641908/140‑1 (MQ=255)

                                                        ccTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGc                                                                       >  1:1044554/1‑140 (MQ=255)

                                                         cTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGcc                                                                      >  2:1392660/1‑140 (MQ=255)

                                                            gTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGaa                                                                   <  2:204744/140‑1 (MQ=255)

                                                               gATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGAc                                                                >  1:740105/1‑140 (MQ=255)

                                                                aTAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACg                                                               >  2:1039163/1‑140 (MQ=255)

                                                                aTAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACg                                                               >  2:532772/1‑140 (MQ=255)

                                                                aTAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACg                                                               >  1:1591144/1‑140 (MQ=255)

                                                                 tAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGc                                                              <  2:304353/140‑1 (MQ=255)

                                                                  aaaGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCa                                                             >  1:1336045/1‑140 (MQ=255)

                                                                    aGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACg                                                           >  1:799389/1‑140 (MQ=255)

                                                                     gggTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGa                                                          <  2:773065/140‑1 (MQ=255)

                                                                      ggTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGAt                                                         <  1:1451953/140‑1 (MQ=255)

                                                                      ggTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGAt                                                         <  2:1591144/140‑1 (MQ=255)

                                                                      ggTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGAt                                                         <  1:1607/140‑1 (MQ=255)

                                                                         ttgtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACg                                                      >  1:1558423/1‑140 (MQ=255)

                                                                          tgtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGa                                                          >  2:389382/1‑135 (MQ=255)

                                                                          tgtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGa                                                          <  1:389382/135‑1 (MQ=255)

                                                                          tgtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGc                                                     >  1:1001504/1‑140 (MQ=255)

                                                                          tgtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGc                                                     >  1:883817/1‑140 (MQ=255)

                                                                          tgtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGc                                                     >  1:1497424/1‑140 (MQ=255)

                                                                           gtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGc                                                     >  2:1114285/1‑139 (MQ=255)

                                                                           gtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGc                                                     <  1:1114285/139‑1 (MQ=255)

                                                                           gtttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCACGGCGAAGATCCAGCCGAAGACGCACGATa                                                        <  2:205434/136‑1 (MQ=255)

                                                                              tgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCt                                                    >  2:772010/1‑137 (MQ=255)

                                                                              tgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCt                                                    <  1:772010/137‑1 (MQ=255)

                                                                                aCATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTcaca                                                <  1:877209/139‑1 (MQ=255)

                                                                                aCATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTcaca                                                >  2:877209/1‑139 (MQ=255)

                                                                                 cATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACAcc                                              >  2:180645/1‑140 (MQ=255)

                                                                                 cATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGAGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACAcc                                              >  2:1433577/1‑140 (MQ=255)

                                                                                  aTAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCa                                             >  1:357495/1‑140 (MQ=255)

                                                                                      cTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGAt                                                         <  1:469207/124‑1 (MQ=255)

                                                                                             ccTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCgg                                  <  2:740105/140‑1 (MQ=255)

                                                                                             ccTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCgg                                  <  1:67543/140‑1 (MQ=255)

                                                                                             ccTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCgg                                  <  2:609261/140‑1 (MQ=255)

                                                                                                   aCTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAAt                            <  1:1305288/140‑1 (MQ=255)

                                                                                                   aCTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAAt                            <  2:357495/140‑1 (MQ=255)

                                                                                                    ctctGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAAc                                       <  2:346905/128‑1 (MQ=255)

                                                                                                    ctctGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAAc                                       >  1:346905/1‑128 (MQ=255)

                                                                                                    ctctGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATa                           <  2:286112/140‑1 (MQ=255)

                                                                                                       tGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAg                        >  2:423146/1‑140 (MQ=255)

                                                                                                       tGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAg                        >  1:393985/1‑140 (MQ=255)

                                                                                                       tGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAg                        <  2:1336045/140‑1 (MQ=255)

                                                                                                       tGTATTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAg                        >  2:928749/1‑140 (MQ=255)

                                                                                                          aTTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtg                     <  1:1101028/140‑1 (MQ=255)

                                                                                                          aTTTGTTATTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCAGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtg                     <  2:1392844/140‑1 (MQ=255)

                                                                                                                 aTTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtg                     <  2:590766/133‑1 (MQ=255)

                                                                                                                 aTTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtg                     >  1:590766/1‑133 (MQ=255)

                                                                                                                 aTTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCt                      <  1:138584/132‑1 (MQ=255)

                                                                                                                 aTTCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtga              >  1:1463302/1‑140 (MQ=255)

                                                                                                                   tCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatg            >  2:400849/1‑140 (MQ=255)

                                                                                                                   tCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatg            >  1:206619/1‑140 (MQ=255)

                                                                                                                   tCGAAAATGGCGTGCCGCGCAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatg            >  1:307372/1‑140 (MQ=255)

GTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAC  >  NC_000913/4347243‑4347507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`