Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,399,779	C→T	F176F (TTC→TTT)	miaA →	tRNA dimethylallyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,399,779	0	C	T	100.0%	311.1 / NA	86	F176F (TTC→TTT)	miaA	tRNA dimethylallyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/47);  total (39/47)

CCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATG  >  NC_000913/4399643‑4399914                                                                                                                                         |                                                                                                                                        ccAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTAtt                                                                                                                                      <  2:1513873/140‑1 (MQ=255)  cAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGGATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTAttt                                                                                                                                     >  1:1565030/1‑140 (MQ=255)  cAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTAttt                                                                                                                                     >  2:313939/1‑140 (MQ=255)    gAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGAACCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCg                                                                                                                                   >  2:1096907/1‑140 (MQ=255)          aGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTaaa                                                                                                                             >  1:1042794/1‑140 (MQ=255)          aGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTaaa                                                                                                                             >  2:1270475/1‑140 (MQ=255)          aGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTaaa                                                                                                                             >  1:767497/1‑140 (MQ=255)          aGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTaaa                                                                                                                             >  2:556883/1‑140 (MQ=255)          aGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTaaa                                                                                                                             >  1:224802/1‑140 (MQ=255)            cAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAAc                                                                                                                           <  2:756026/140‑1 (MQ=255)            cAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAAc                                                                                                                           >  2:575111/1‑140 (MQ=255)               cAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACttt                                                                                                                        >  2:730402/1‑140 (MQ=255)               cAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTC‑GGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTa                                                                                                                       >  2:1372213/1‑140 (MQ=255)                    ggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACgg                                                                                                                   <  2:1181486/140‑1 (MQ=255)                              ggTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCa                                                                                                         >  1:132109/1‑140 (MQ=255)                              ggTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCa                                                                                                         <  1:445788/140‑1 (MQ=255)                               gTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCaa                                                                                                        <  2:610821/140‑1 (MQ=255)                                ttGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCaaa                                                                                                       <  2:767497/140‑1 (MQ=255)                                   ggAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGt                                                                                                    >  2:913584/1‑140 (MQ=255)                                   ggAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGt                                                                                                    >  2:1140550/1‑140 (MQ=255)                                    gAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTc                                                                                                   <  1:1270475/140‑1 (MQ=255)                                       tCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAgg                                                                                                <  2:1183244/140‑1 (MQ=255)                                        cATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGga                                                                                               >  1:372450/1‑140 (MQ=255)                                         aTTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGgag                                                                                              <  1:190780/140‑1 (MQ=255)                                          ttGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGgaga                                                                                             >  2:475172/1‑140 (MQ=255)                                          ttGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGgaga                                                                                             <  2:35319/140‑1 (MQ=255)                                          ttGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGgaga                                                                                             <  1:1532541/140‑1 (MQ=255)                                           tGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGAc                                                                                            >  1:1200710/1‑140 (MQ=255)                                            ggaTCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACg                                                                                           <  1:913584/138‑1 (MQ=255)                                            ggaTCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACg                                                                                           <  2:1565030/138‑1 (MQ=255)                                            gCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACg                                                                                           <  2:658413/140‑1 (MQ=255)                                            gCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACg                                                                                           <  2:1200710/140‑1 (MQ=255)                                             cATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGc                                                                                          <  1:1067763/140‑1 (MQ=255)                                             cATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGc                                                                                          <  1:849572/140‑1 (MQ=255)                                                cGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGctct                                                                                       <  2:372450/140‑1 (MQ=255)                                                 gTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTa                                                                                      <  2:729391/140‑1 (MQ=255)                                                  tCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTAc                                                                                     >  2:1200024/1‑140 (MQ=255)                                                  tCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTAc                                                                                     >  2:621642/1‑140 (MQ=255)                                                  tCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTAc                                                                                     >  1:842301/1‑140 (MQ=255)                                                    aaCTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGt                                                                                                    >  1:1331074/1‑123 (MQ=255)                                                    aaCTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGt                                                                                                    <  2:1331074/123‑1 (MQ=255)                                                        tCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATc                                                                               <  1:730402/140‑1 (MQ=255)                                                           ggaggTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAgg                                                                            <  1:1163860/140‑1 (MQ=255)                                                            gaggTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGt                                                                           >  2:1301834/1‑140 (MQ=255)                                                                tAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCAt                                                                       >  2:35580/1‑140 (MQ=255)                                                                tAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCAt                                                                       <  2:596152/140‑1 (MQ=255)                                                                tAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCAt                                                                       >  2:667907/1‑140 (MQ=255)                                                                   aTCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAg                                                                    >  1:1013310/1‑140 (MQ=255)                                                                     ccGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGtt                                                                  <  1:1372213/140‑1 (MQ=255)                                                                     ccGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGtt                                                                  <  1:1301834/140‑1 (MQ=255)                                                                        gTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGc                                                               >  1:993745/1‑140 (MQ=255)                                                                        gTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGc                                                               >  2:478253/1‑140 (MQ=255)                                                                          tGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCa                                                             <  1:131066/140‑1 (MQ=255)                                                                          tGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCa                                                             >  2:1122954/1‑140 (MQ=255)                                                                          tGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCa                                                             >  1:11408/1‑140 (MQ=255)                                                                          tGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCa                                                             <  1:621642/140‑1 (MQ=255)                                                                          tGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCa                                                             >  1:1509191/1‑140 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCTCTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  2:1013310/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  2:29749/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  2:993745/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  1:1096907/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  2:11408/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  1:679318/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  1:556883/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  1:313939/140‑1 (MQ=255)                                                                                    aGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                                                   <  1:35580/140‑1 (MQ=255)                                                                                           aTCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCgg                                            >  2:1122859/1‑139 (MQ=255)                                                                                           aTCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGt                                            >  1:1322284/1‑140 (MQ=255)                                                                                            tCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTg                                           <  2:132109/140‑1 (MQ=255)                                                                                             ccAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGTAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCGTCGCCCCGGCGAGCCGTGa                                          <  1:575111/140‑1 (MQ=255)                                                                                                       cacaAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCAt                                >  1:93148/1‑140 (MQ=255)                                                                                                         caAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCa                              <  2:665681/140‑1 (MQ=255)                                                                                                         caAAGGCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCa                              <  1:475172/140‑1 (MQ=255)                                                                                                              gCTTTCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCa                         >  1:897405/1‑140 (MQ=255)                                                                                                                  tCCCGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCACCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGa                     >  1:707033/1‑140 (MQ=255)                                                                                                                   cccGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGag                    <  2:1540105/140‑1 (MQ=255)                                                                                                                     cGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGagca                  <  2:842301/140‑1 (MQ=255)                                                                                                                     cGGGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGagca                  <  2:93148/140‑1 (MQ=255)                                                                                                                        gCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAATTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCg               <  1:389412/140‑1 (MQ=255)                                                                                                                        gCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCg               <  1:256102/140‑1 (MQ=255)                                                                                                                        gCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCg               <  1:1125298/140‑1 (MQ=255)                                                                                                                            tGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGtttt           >  1:281508/1‑140 (MQ=255)                                                                                                                            tGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGtttt           >  2:707145/1‑140 (MQ=255)                                                                                                                              gAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGagc                   >  1:1414546/1‑130 (MQ=255)                                                                                                                              gAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGagc                   <  2:1414546/130‑1 (MQ=255)                                                                                                                                 gTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTtcatca      >  2:583461/1‑140 (MQ=255)                                                                                                                                    ttttttATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGAt   >  1:1350158/1‑140 (MQ=255)                                                                                                                                     ttttTATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATg  <  2:1042794/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATG  >  NC_000913/4399643‑4399914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑