Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,507	A→G	R13R (CGT→CGC)	ampG ←	muropeptide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,507	0	A	G	100.0%	205.5 / NA	57	R13R (CGT→CGC)	ampG	muropeptide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/25);  total (32/25)

GGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATC  >  NC_000913/453377‑453642                                                                                                                                   |                                                                                                                                        ggCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGt                                                                                                                                >  1:1477442/1‑140 (MQ=255)       ccAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGaaaa                                                                                                                          >  2:299308/1‑140 (MQ=255)             agaagaAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGt                                                                                                                    <  1:970665/140‑1 (MQ=255)             agaagaAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGt                                                                                                                    >  2:980486/1‑140 (MQ=255)             agaagaAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGt                                                                                                                    <  1:881804/140‑1 (MQ=255)               aagaaACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTaa                                                                                                                  >  1:1527897/1‑140 (MQ=255)                      cAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGa                                                                                                           <  2:581395/140‑1 (MQ=255)                      cAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGa                                                                                                           <  1:325296/140‑1 (MQ=255)                           gTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATa                                                                                                                       <  2:1002585/123‑1 (MQ=255)                           gTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATa                                                                                                                       >  1:1002585/1‑123 (MQ=255)                                      gATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACa                                                                                                    <  1:1331535/131‑1 (MQ=255)                                      gATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACa                                                                                                    >  2:1331535/1‑131 (MQ=255)                                      gATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCt                                                                                           >  1:979778/1‑140 (MQ=255)                                      gATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCt                                                                                           >  1:443995/1‑140 (MQ=255)                                            ctcGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGc                                                                                     <  2:137309/140‑1 (MQ=255)                                              cGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGccc                                                                                   <  2:1527897/140‑1 (MQ=255)                                                    tCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTg                                                                             >  2:1562824/1‑140 (MQ=255)                                                              cTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCa                                                                   <  1:980486/140‑1 (MQ=255)                                                               tGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCATCGGGCCag                                                                  >  1:1004007/1‑140 (MQ=255)                                                               tGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCag                                                                  >  1:850719/1‑140 (MQ=255)                                                                 tAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCagag                                                                >  1:437943/1‑140 (MQ=255)                                                                 tAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCagag                                                                >  1:1532494/1‑140 (MQ=255)                                                                 tAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCagag                                                                >  1:1603706/1‑140 (MQ=255)                                                                      tACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAg                                                           >  1:1589807/1‑140 (MQ=255)                                                                         cGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGaaa                                                        >  2:1073066/1‑140 (MQ=255)                                                                         cGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGaaa                                                        >  2:1190037/1‑140 (MQ=255)                                                                          ggATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAAt                                                       >  2:1112357/1‑140 (MQ=255)                                                                           gATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAAtt                                                      >  1:52876/1‑140 (MQ=255)                                                                           gATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAAtt                                                      <  2:1589807/140‑1 (MQ=255)                                                                             tGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTaa                                                    >  1:616726/1‑140 (MQ=255)                                                                                cAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAAcgc                                                 >  1:457166/1‑140 (MQ=255)                                                                                   ggCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAAcgcgcg                                              >  2:780775/1‑140 (MQ=255)                                                                                     cGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGtt                                            <  2:437943/140‑1 (MQ=255)                                                                                     cGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGtt                                            <  1:299308/140‑1 (MQ=255)                                                                                     cGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGtt                                            <  2:1477442/140‑1 (MQ=255)                                                                                      gAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTc                                           >  2:1146635/1‑140 (MQ=255)                                                                                           gTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCtt                                      <  2:616726/140‑1 (MQ=255)                                                                                             aGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTtga                                    <  1:1316224/140‑1 (MQ=255)                                                                                              gCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTtgat                                   <  1:1148481/140‑1 (MQ=255)                                                                                                  ggAAGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAAt                               >  2:1218108/1‑140 (MQ=255)                                                                                                    aaGCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTc                             <  1:1146635/140‑1 (MQ=255)                                                                                                      gCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGt                           <  2:457166/140‑1 (MQ=255)                                                                                                      gCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGt                           >  2:1194019/1‑140 (MQ=255)                                                                                                      gCAAAACCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGt                           <  2:1322074/140‑1 (MQ=255)                                                                                                         aaaCCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGa                        >  1:1015373/1‑140 (MQ=255)                                                                                                         aaaCCCAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGa                        <  1:1073066/140‑1 (MQ=255)                                                                                                              cAGGATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTg                   <  2:1015373/140‑1 (MQ=255)                                                                                                                 gATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTATAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGgtg                >  1:1349317/1‑140 (MQ=255)                                                                                                                 gATCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGgtg                <  1:1562824/140‑1 (MQ=255)                                                                                                                  aTCAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGgtgt               >  2:782056/1‑140 (MQ=255)                                                                                                                    cAGCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTcc             <  1:1190037/140‑1 (MQ=255)                                                                                                                      gCAATATGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTg           <  2:1603706/140‑1 (MQ=255)                                                                                                                            tGGCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACat     >  1:351324/1‑140 (MQ=255)                                                                                                                             ggCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTgaga        <  2:74794/136‑1 (MQ=255)                                                                                                                             ggCTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTgaga        >  1:74794/1‑136 (MQ=255)                                                                                                                               cTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATc  >  2:1570607/1‑140 (MQ=255)                                                                                                                               cTGAGCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATc  >  1:1425223/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        GGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATC  >  NC_000913/453377‑453642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑