Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,944	C→T	intergenic (‑94/+22)	yjiM ← / ← yjiN	putative dehydratase subunit/DUF445 domain‑containing protein YjiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,944	0	C	T	100.0%	319.3 / NA	90	intergenic (‑94/+22)	yjiM/yjiN	putative dehydratase subunit/DUF445 domain‑containing protein YjiN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/41);  total (49/41)

GAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCG  >  NC_000913/4565809‑4566080                                                                                                                                        |                                                                                                                                         gaACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACt                                                                                                                                      <  2:214103/140‑1 (MQ=255)    cTGATCGAAAATGGCGGGTAGATCGGGGACAAATGACATGATTTTTCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAg                                                                                                                                   >  1:549758/1‑140 (MQ=255)       aTCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGttt                                                                                                                                >  2:722012/1‑140 (MQ=255)       aTCGAAAATGGCGGGGAGATCGGTGACAAGTGACATGATTTTTCTTCCCCCTACCACGGGGGATAATGAAAAAAGGCCACATTATTTCCATTCTTTTAGTATGGTTTATTTTATCTCTCTCGCGGTTAGTCACTTTGttt                                                                                                                                >  2:1011168/1‑140 (MQ=255)       aTCGAAAATGGCGGGGAAACCGGGGTCAAAGGGCATGCTTTTCATTCCCCGGACCACGGGGGAGAATGAAAAGAGGCCACATTATTAACATTCTTTTTGTATGGTTTATTTTATCTCTCTTGCGGTTAGTCACTTAGttt                                                                                                                                >  2:852446/1‑140 (MQ=255)                    ggTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCt                                                                                                                   <  2:16387/140‑1 (MQ=255)                                   aGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAggg                                                                                                    >  2:283731/1‑140 (MQ=255)                                    gTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgggg                                                                                                   >  1:924597/1‑140 (MQ=255)                                      gACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGa                                                                                                 <  2:56934/140‑1 (MQ=255)                                       aCATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGaa                                                                                                <  2:639358/140‑1 (MQ=255)                                       aCATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGaa                                                                                                >  2:234913/1‑140 (MQ=255)                                         aTGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACa                                                                                              <  1:283731/140‑1 (MQ=255)                                          tGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGTGGAACaa                                                                                             >  2:1268488/1‑140 (MQ=255)                                               ttccttccCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCg                                                                                        >  1:451848/1‑140 (MQ=255)                                                     ccccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTCTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCt                                                                                  >  2:526551/1‑140 (MQ=255)                                                      cccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGaa                                                                                                <  1:420214/125‑1 (MQ=255)                                                      cccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGaa                                                                                                >  2:420214/1‑125 (MQ=255)                                                             cACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                          <  1:722012/140‑1 (MQ=255)                                                               cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCTGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                                                        >  1:1093316/1‑140 (MQ=255)                                                               cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                                                        >  2:720303/1‑140 (MQ=255)                                                               cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                                                        >  1:1154018/1‑140 (MQ=255)                                                                gggAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaaa                                                                       >  1:625195/1‑140 (MQ=255)                                                                 ggAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAat                                                                      <  2:451848/140‑1 (MQ=255)                                                                    gaTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAAtata                                                                   >  2:1331926/1‑140 (MQ=255)                                                                     aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAat                                                                      <  1:378623/136‑1 (MQ=255)                                                                     aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAat                                                                      >  2:378623/1‑136 (MQ=255)                                                                     aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATaa                                                                  >  2:930811/1‑140 (MQ=255)                                                                       aaTGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATaaaa                                                                >  1:1148177/1‑140 (MQ=255)                                                                       aaTGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATaaaa                                                                >  1:283175/1‑140 (MQ=255)                                                                        aTGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                               <  2:283175/140‑1 (MQ=255)                                                                        aTGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                               <  1:706850/140‑1 (MQ=255)                                                                          gAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTa                                                             >  2:265178/1‑140 (MQ=255)                                                                           aaaaGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                            <  2:549758/140‑1 (MQ=255)                                                                            aaaGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGc                                                           >  2:846135/1‑140 (MQ=255)                                                                            aaaGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGc                                                           >  1:730112/1‑140 (MQ=255)                                                                              agagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                                                         >  1:1227333/1‑140 (MQ=255)                                                                              agagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                                                         >  1:1037664/1‑140 (MQ=255)                                                                              agagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                                                         <  2:1227333/140‑1 (MQ=255)                                                                              agagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                                                         >  1:1539466/1‑140 (MQ=255)                                                                               gagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCa                                                        >  1:971573/1‑140 (MQ=255)                                                                                agGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                            >  1:1389195/1‑135 (MQ=255)                                                                                agGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                            <  2:1389195/135‑1 (MQ=255)                                                                                agGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCaa                                                       >  1:481076/1‑140 (MQ=255)                                                                                agGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCaa                                                       >  2:131775/1‑140 (MQ=255)                                                                                  gCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGTGACAGCAAATATAAAATTAGCCCAATa                                                     >  2:1345539/1‑140 (MQ=255)                                                                                   ccACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                    <  1:846135/140‑1 (MQ=255)                                                                                          attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                             <  1:930811/140‑1 (MQ=255)                                                                                          attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                             <  2:730112/140‑1 (MQ=255)                                                                                          attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                             <  2:481076/140‑1 (MQ=255)                                                                                          attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                             >  1:1518691/1‑140 (MQ=255)                                                                                            taCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCTGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                           <  2:1093316/140‑1 (MQ=255)                                                                                             aCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          <  2:343294/140‑1 (MQ=255)                                                                                             aCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          <  1:234913/140‑1 (MQ=255)                                                                                             aCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          <  2:1204561/140‑1 (MQ=255)                                                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc                                      <  1:1200381/140‑1 (MQ=255)                                                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc                                      >  1:343825/1‑140 (MQ=255)                                                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc                                      >  1:1551150/1‑140 (MQ=255)                                                                                                  tCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                     <  1:852446/140‑1 (MQ=255)                                                                                                  tCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                     <  2:343825/140‑1 (MQ=255)                                                                                                     tttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          <  1:1364207/132‑1 (MQ=255)                                                                                                     tttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          >  2:1364207/1‑132 (MQ=255)                                                                                                      ttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTAcc                                 >  2:1371256/1‑140 (MQ=255)                                                                                                            tGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGac                           >  1:1300646/1‑140 (MQ=255)                                                                                                            tGGTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGac                           >  2:1146052/1‑140 (MQ=255)                                                                                                             ggTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAATAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCa                                        <  2:1396007/126‑1 (MQ=255)                                                                                                             ggTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCa                                        >  1:1396007/1‑126 (MQ=255)                                                                                                              gTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          <  1:1021660/123‑1 (MQ=255)                                                                                                              gTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                          >  2:1021660/1‑123 (MQ=255)                                                                                                              gTTTATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGacac                         >  2:188379/1‑140 (MQ=255)                                                                                                                 tATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGaa                      <  1:188379/140‑1 (MQ=255)                                                                                                                 tATTTGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGaa                      >  2:746476/1‑140 (MQ=255)                                                                                                                   tttGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                    <  2:924597/140‑1 (MQ=255)                                                                                                                   tttGATCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                    <  2:1037664/140‑1 (MQ=255)                                                                                                                       aTCTCTCTCGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACt                >  2:381507/1‑140 (MQ=255)                                                                                                                        tctctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                    >  2:100189/1‑135 (MQ=255)                                                                                                                        tctctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                    <  1:100189/135‑1 (MQ=255)                                                                                                                        tctctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTg               >  2:156277/1‑140 (MQ=255)                                                                                                                         ctctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGc              >  1:95531/1‑140 (MQ=255)                                                                                                                         ctctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGc              >  1:1437847/1‑140 (MQ=255)                                                                                                                         ctctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGc              >  2:1527740/1‑140 (MQ=255)                                                                                                                           ctctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAg            >  2:1452308/1‑140 (MQ=255)                                                                                                                             ctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCTGCGTACCGTTGACACGAATAAACTGCAGAt          >  2:984699/1‑140 (MQ=255)                                                                                                                             ctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGAt          >  1:449335/1‑140 (MQ=255)                                                                                                                             ctcGCGGTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGAt          >  1:286561/1‑140 (MQ=255)                                                                                                                                   gTTAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGc    <  2:1300646/140‑1 (MQ=255)                                                                                                                                    ttAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGcc   <  1:359780/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGTGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  1:1268488/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  2:1154018/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  2:286561/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  1:891705/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  1:54431/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  2:1551150/140‑1 (MQ=255)                                                                                                                                     tAGTCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg  <  1:1331926/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCG  >  NC_000913/4565809‑4566080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑