Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	751,199	T→C	N324S (AAC→AGC)	ybgQ ←	putative fimbrial usher protein YbgQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	751,199	0	T	C	100.0%	347.6 / NA	96	N324S (AAC→AGC)	ybgQ	putative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/51);  total (45/51)

TTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCG  >  NC_000913/751066‑751334                                                                                                                                      |                                                                                                                                        ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCAccc                                                                                                                                   >  2:1240370/1‑140 (MQ=255) ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCAccc                                                                                                                                   >  2:1171615/1‑140 (MQ=255) ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCAccc                                                                                                                                   >  1:42712/1‑140 (MQ=255)   cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGa                                                                                                                                 <  1:96169/140‑1 (MQ=255)      tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGa                                                                                                                                 >  2:1302730/1‑137 (MQ=255)      tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGa                                                                                                                                 <  1:1302730/137‑1 (MQ=255)      tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCa                                                                                                                              >  1:63299/1‑140 (MQ=255)             aaaGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATcttctt                                                                                                                       >  2:1588556/1‑140 (MQ=255)                ggTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCTGGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCg                                                                                                                    <  2:1432151/140‑1 (MQ=255)                   ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCa                                                                                                                 <  2:42712/140‑1 (MQ=255)                   ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCa                                                                                                                 <  1:1456130/140‑1 (MQ=255)                        tttCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGCCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTg                                                                                                            >  2:45522/1‑140 (MQ=255)                          tCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGAc                                                                                                          <  2:889025/140‑1 (MQ=255)                            aGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACAt                                                                                                        <  1:1171615/140‑1 (MQ=255)                            aGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACAt                                                                                                        <  1:565129/140‑1 (MQ=255)                              tttGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATcc                                                                                                      >  2:1173793/1‑140 (MQ=255)                                 gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                                                   <  2:1040896/140‑1 (MQ=255)                                 gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                                                   <  1:1225372/140‑1 (MQ=255)                                 gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                                                   <  2:961714/140‑1 (MQ=255)                                 gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                                                   <  2:14652/140‑1 (MQ=255)                                   tgtgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGt                                                                                                 >  2:156802/1‑140 (MQ=255)                                     tgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGc                                                                                               <  1:1240370/140‑1 (MQ=255)                                      gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGcc                                                                                              <  1:1229564/140‑1 (MQ=255)                                           ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                                                         <  2:1363283/140‑1 (MQ=255)                                           ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                                                         <  1:1058928/140‑1 (MQ=255)                                            gAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAAc                                                                                        >  1:881481/1‑140 (MQ=255)                                               ggCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa                                                                                     <  1:1532675/140‑1 (MQ=255)                                               ggCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa                                                                                     <  2:1252070/140‑1 (MQ=255)                                               ggCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa                                                                                     <  2:1151568/140‑1 (MQ=255)                                                gCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGAc                                                                                    <  2:1299456/140‑1 (MQ=255)                                                 ccGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt                                                                                   >  1:164246/1‑140 (MQ=255)                                                 ccGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt                                                                                   >  2:1310878/1‑140 (MQ=255)                                                  cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                                                   >  2:584681/1‑123 (MQ=255)                                                  cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                                                   <  1:584681/123‑1 (MQ=255)                                                  cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTg                                                                                  >  1:194946/1‑140 (MQ=255)                                                  cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTg                                                                                  >  1:767911/1‑140 (MQ=255)                                                  cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTg                                                                                  >  2:997065/1‑140 (MQ=255)                                                   gTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCTAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTg                                                                                           <  2:314013/130‑1 (MQ=255)                                                   gTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTg                                                                                           >  1:314013/1‑130 (MQ=255)                                                    tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAg                                                                                      >  1:1290056/1‑134 (MQ=255)                                                    tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAg                                                                                      <  2:1290056/134‑1 (MQ=255)                                                    tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGAt                                                                                >  1:1275108/1‑140 (MQ=255)                                                    tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGAt                                                                                >  2:81301/1‑140 (MQ=255)                                                      gCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTg                                                                              <  1:594936/140‑1 (MQ=255)                                                      gCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTg                                                                              >  2:321736/1‑140 (MQ=255)                                                        tGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGTGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAg                                                                            <  1:596088/140‑1 (MQ=255)                                                        tGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAg                                                                            <  2:82836/140‑1 (MQ=255)                                                          aCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTg                                                                              <  2:410893/136‑1 (MQ=255)                                                          aCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTg                                                                              >  1:410893/1‑136 (MQ=255)                                                           cccGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtc                                                                         <  1:45522/140‑1 (MQ=255)                                                             cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                                                       <  2:1123129/140‑1 (MQ=255)                                                             cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                                                       <  2:1514313/140‑1 (MQ=255)                                                             cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                                                       <  1:1173793/140‑1 (MQ=255)                                                              gcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatc                                                                      <  1:96017/140‑1 (MQ=255)                                                              gcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatc                                                                      <  2:1225891/140‑1 (MQ=255)                                                              gcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatc                                                                      >  2:399883/1‑140 (MQ=255)                                                               cgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatca                                                                     >  1:923332/1‑140 (MQ=255)                                                                  ccAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCaata                                                                  <  2:164246/140‑1 (MQ=255)                                                                       ttATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATaaa                                                             <  1:1310878/140‑1 (MQ=255)                                                                           aGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACggg                                                         <  2:1275108/140‑1 (MQ=255)                                                                              gAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCt                                                      >  2:528554/1‑140 (MQ=255)                                                                               aaCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTg                                                     >  2:1342718/1‑140 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATaaa                                                             <  2:215418/131‑1 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATaaa                                                             >  1:215418/1‑131 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACggg                                                         <  2:1350038/135‑1 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACggg                                                         >  1:1350038/1‑135 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTgg                                                    >  1:1216936/1‑140 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTgg                                                    >  2:952380/1‑140 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTgg                                                    >  1:475968/1‑140 (MQ=255)                                                                                aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTgg                                                    >  1:731686/1‑140 (MQ=255)                                                                                 ccTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGc                                                   >  1:523572/1‑140 (MQ=255)                                                                                  cTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCg                                                  <  2:881481/140‑1 (MQ=255)                                                                                    gTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCggg                                                >  2:75814/1‑140 (MQ=255)                                                                                     tCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGa                                               >  2:79121/1‑140 (MQ=255)                                                                                     tCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGa                                               >  1:244526/1‑140 (MQ=255)                                                                                          ggCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACtttt                                          <  2:523572/140‑1 (MQ=255)                                                                                          ggCGAGCCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACtttt                                          >  2:852598/1‑140 (MQ=255)                                                                                              aGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCt                                      >  1:269834/1‑140 (MQ=255)                                                                                               gTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTg                                     >  1:1261679/1‑140 (MQ=255)                                                                                                tCAGGAAGGGCGTCGTTGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTgg                                    >  1:650434/1‑140 (MQ=255)                                                                                                       gggCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGa                             <  2:923332/140‑1 (MQ=255)                                                                                                         gCGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAcg                           <  2:194946/140‑1 (MQ=255)                                                                                                          cGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGAGTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAcgc                          >  2:1404440/1‑140 (MQ=255)                                                                                                          cGTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAcgc                          >  1:909466/1‑140 (MQ=255)                                                                                                           gTCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAcgcg                         <  2:63299/140‑1 (MQ=255)                                                                                                            tCGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCgg                        >  2:949387/1‑140 (MQ=255)                                                                                                             cGATGCTGCCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGc                       <  2:475968/140‑1 (MQ=255)                                                                                                                   tgcCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGc                       <  2:209362/134‑1 (MQ=255)                                                                                                                   tgcCGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACCTTTTTCTGGTAAATGACGCGGc                       >  1:209362/1‑134 (MQ=255)                                                                                                                     ccGAAACCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGa               <  2:1216936/140‑1 (MQ=255)                                                                                                                      cGAAACCTGGAAATTGCTCACCCGACCATCTACTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGAc              <  1:68669/140‑1 (MQ=255)                                                                                                                          aCCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAc                            <  2:337906/122‑1 (MQ=255)                                                                                                                          aCCTGGAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAc                            >  1:337906/1‑122 (MQ=255)                                                                                                                               gAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGa               >  1:251885/1‑130 (MQ=255)                                                                                                                               gAAATTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGa               <  2:251885/130‑1 (MQ=255)                                                                                                                                  aTTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCg  <  2:1057542/140‑1 (MQ=255)                                                                                                                                  aTTGCTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCg  <  2:767911/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCG  >  NC_000913/751066‑751334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑