Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,010,898	C→T	A312V (GCC→GTC)	uup →	ATP‑binding protein with possible role in replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,010,898	0	C	T	100.0%	180.2 / NA	52	A312V (GCC→GTC)	uup	ATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

TGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGC  >  NC_000913/1010768‑1011031                                                                                                                                    |                                                                                                                                      tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTc                                                                                                                              >  2:266668/1‑140 (MQ=255)         ccAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTaaaa                                                                                                                      >  2:1561287/1‑140 (MQ=255)         ccAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTaaaa                                                                                                                      >  1:637274/1‑140 (MQ=255)                aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGtttt                                                                                                               >  2:434059/1‑140 (MQ=255)                aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGtttt                                                                                                               <  1:1561287/140‑1 (MQ=255)                        aCGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATgg                                                                                                       >  2:995434/1‑140 (MQ=255)                         cGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGa                                                                                                      >  1:270350/1‑140 (MQ=255)                           ccGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAg                                                                                                    <  1:434059/140‑1 (MQ=255)                               aCCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt                                                                                                <  1:677599/140‑1 (MQ=255)                               aCCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt                                                                                                >  2:893823/1‑140 (MQ=255)                                  cGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTg                                                                                             <  2:637274/140‑1 (MQ=255)                                      aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTGCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         <  1:1353555/140‑1 (MQ=255)                                      aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         <  1:1003035/140‑1 (MQ=255)                                      aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         <  1:484319/140‑1 (MQ=255)                                         aaGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         >  2:1499269/1‑137 (MQ=255)                                         aaGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         <  1:1499269/137‑1 (MQ=255)                                           ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                                    >  2:1423961/1‑140 (MQ=255)                                           ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                                    <  1:1454472/140‑1 (MQ=255)                                           ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCACTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                                    >  2:272827/1‑140 (MQ=255)                                             ccGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTa                                                                                  >  2:820544/1‑140 (MQ=255)                                              cgcgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCACTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTaa                                                                                 <  1:272827/140‑1 (MQ=255)                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         >  2:1463287/1‑130 (MQ=255)                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                         <  1:1463287/130‑1 (MQ=255)                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAgg                                                                                     <  2:1563737/134‑1 (MQ=255)                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAgg                                                                                     >  1:1563737/1‑134 (MQ=255)                                                        cccTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCaa                                                                       <  2:436308/140‑1 (MQ=255)                                                              aaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTg                                                                 <  2:1261995/140‑1 (MQ=255)                                                               aGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTg                                                                 <  2:1341337/140‑1 (MQ=255)                                                                   aTGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAC‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGa                                                            >  1:998722/1‑140 (MQ=255)                                                                        tCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAAc                                                                      <  1:1377018/125‑1 (MQ=255)                                                                        tCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAAc                                                                      >  2:1377018/1‑125 (MQ=255)                                                                             aaCGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                  <  1:893823/140‑1 (MQ=255)                                                                             aaCGTGGTGAACGTCGCGAAGTGAT‑AGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                  <  2:897801/140‑1 (MQ=255)                                                                                    tgAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTc                                           <  1:266668/140‑1 (MQ=255)                                                                                     gAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCt                                          >  2:293795/1‑140 (MQ=255)                                                                                         gTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGt                                      <  1:178819/140‑1 (MQ=255)                                                                                          tCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTg                                     >  1:250713/1‑140 (MQ=255)                                                                                                   gAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACaaaa                            <  1:845830/140‑1 (MQ=255)                                                                                                    aT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAAt                           >  1:77634/1‑140 (MQ=255)                                                                                                             cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                   >  2:1444465/1‑140 (MQ=255)                                                                                                             cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                   >  1:957478/1‑140 (MQ=255)                                                                                                             cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                   >  1:1375311/1‑140 (MQ=255)                                                                                                              gCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGAt                  <  2:147760/140‑1 (MQ=255)                                                                                                              gCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGAt                  <  2:1426356/140‑1 (MQ=255)                                                                                                                   gATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTc             >  2:522406/1‑140 (MQ=255)                                                                                                                       cAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGaa         <  1:1444465/140‑1 (MQ=255)                                                                                                                       cAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGaa         >  1:1337072/1‑140 (MQ=255)                                                                                                                        aGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAAt        >  1:1111401/1‑140 (MQ=255)                                                                                                                         ggTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATg       >  1:1378111/1‑140 (MQ=255)                                                                                                                           tGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATggg     <  1:293795/140‑1 (MQ=255)                                                                                                                             gAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTg   >  2:911895/1‑140 (MQ=255)                                                                                                                              aaGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGc  >  1:525409/1‑140 (MQ=255)                                                                                                                              aaGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGc  >  1:1176926/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      TGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAT‑GGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGC  >  NC_000913/1010768‑1011031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑