Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,283,745	C→T	P48S (CCG→TCG)	narH →	nitrate reductase A subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,283,745	0	C	T	100.0%	291.2 / NA	81	P48S (CCG→TCG)	narH	nitrate reductase A subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/41);  total (40/41)

GTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCT  >  NC_000913/1283614‑1283873                                                                                                                                    |                                                                                                                                 gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAg                                                                                                                          <  1:1300032/140‑1 (MQ=255)  ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAgg                                                                                                                         >  2:490580/1‑140 (MQ=255)   tCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAggg                                                                                                                        <  2:483367/140‑1 (MQ=255)     acaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCt                                                                                                                      <  1:1228167/140‑1 (MQ=255)      caAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCtt                                                                                                                     <  2:877245/140‑1 (MQ=255)           cGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCga                                                                                                                <  2:240226/140‑1 (MQ=255)           cGGCATGGTGCTGAATCTCGATAAGTACATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCga                                                                                                                <  1:84034/140‑1 (MQ=255)               aTGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCgactga                                                                                                            <  2:937136/140‑1 (MQ=255)               aTGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCgactga                                                                                                            <  2:392668/140‑1 (MQ=255)                 ggTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCgactgact                                                                                                          >  1:288236/1‑140 (MQ=255)                   tGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCg                                                                                                                 >  2:1376111/1‑131 (MQ=255)                   tGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCg                                                                                                                 <  1:1376111/131‑1 (MQ=255)                   tGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTgg                                                                                                        >  1:726300/1‑140 (MQ=255)                   tGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTgg                                                                                                        >  1:5598/1‑140 (MQ=255)                   tGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTgg                                                                                                        >  1:931691/1‑140 (MQ=255)                     cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCga                                                                                                                <  2:729423/130‑1 (MQ=255)                     cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCga                                                                                                                >  1:729423/1‑130 (MQ=255)                          tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAAcc                                                                                                 <  2:124446/140‑1 (MQ=255)                             cGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAgg                                                                                              >  1:411271/1‑140 (MQ=255)                                  aGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGaaaaa                                                                                         >  2:1240699/1‑140 (MQ=255)                                    tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAgg                                                                                              <  2:495903/133‑1 (MQ=255)                                    tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAgg                                                                                              >  1:495903/1‑133 (MQ=255)                                     gCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATAc                                                                                      <  1:382627/140‑1 (MQ=255)                                      cATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACa                                                                                     <  1:526253/140‑1 (MQ=255)                                         cGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAg                                                                                  <  2:777283/140‑1 (MQ=255)                                         cGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTAGGAAAACCAGGAAAAATACAAAg                                                                                  <  1:1295104/140‑1 (MQ=255)                                              gCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAggcggc                                                                             <  2:85430/140‑1 (MQ=255)                                              gCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAggcggc                                                                             <  2:121351/140‑1 (MQ=255)                                                cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGa                                                                                             >  1:20412/1‑122 (MQ=255)                                                cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGa                                                                                             <  2:20412/122‑1 (MQ=255)                                                 acacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTgg                                                                          <  2:726300/140‑1 (MQ=255)                                                     cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATcc                                                                      >  2:225281/1‑140 (MQ=255)                                                     cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATcc                                                                      >  1:700875/1‑140 (MQ=255)                                                      tGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCg                                                                     >  2:870754/1‑140 (MQ=255)                                                        ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTa                                                                   >  1:673866/1‑140 (MQ=255)                                                          cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTaaa                                                                 <  2:716767/140‑1 (MQ=255)                                                                  tGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACgg                                                         >  2:954874/1‑140 (MQ=255)                                                                  tGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACgg                                                         >  1:277941/1‑140 (MQ=255)                                                                  tGTAAAAACGTCTGGACCAGCCGTGAA‑GCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGc                                                        >  2:1420811/1‑140 (MQ=255)                                                                    tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCa                                                       >  1:1102192/1‑140 (MQ=255)                                                                     aaaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCaa                                                      >  1:1586707/1‑140 (MQ=255)                                                                           gTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCa                                                >  1:61234/1‑140 (MQ=255)                                                                            tCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAg                                               <  2:231255/140‑1 (MQ=255)                                                                            tCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAg                                               <  2:5598/140‑1 (MQ=255)                                                                              tGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGcc                                             >  1:1171039/1‑140 (MQ=255)                                                                              tGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGATTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGcc                                             >  2:1410633/1‑140 (MQ=255)                                                                                gACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCcgc                                           <  2:700875/140‑1 (MQ=255)                                                                                 aCCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCcgcg                                          <  2:1082400/140‑1 (MQ=255)                                                                                 aCCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCcgcg                                          <  1:1410633/140‑1 (MQ=255)                                                                                  ccAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCgcgc                                         <  1:1387951/140‑1 (MQ=255)                                                                                     gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATg                                      >  2:1216519/1‑140 (MQ=255)                                                                                     gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATg                                      >  2:1046149/1‑140 (MQ=255)                                                                                      ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATgg                                     <  2:277941/140‑1 (MQ=255)                                                                                          gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTaa                                 >  1:1183175/1‑140 (MQ=255)                                                                                          gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTaa                                 <  1:1046149/140‑1 (MQ=255)                                                                                          gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTaa                                 <  2:288236/140‑1 (MQ=255)                                                                                          gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTaa                                 >  1:792978/1‑140 (MQ=255)                                                                                            aGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAAcc                               <  2:411271/140‑1 (MQ=255)                                                                                               cGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTg                            >  2:600392/1‑140 (MQ=255)                                                                                                gTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGc                           >  1:473658/1‑140 (MQ=255)                                                                                                 tGGAATATGCGTGGTTCAACAACGTGGAAACCAAGTCTGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGcc                          >  1:1603336/1‑140 (MQ=255)                                                                                                  ggAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCa                         <  1:1074878/140‑1 (MQ=255)                                                                                                   gAATATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCAt                        >  1:1138554/1‑140 (MQ=255)                                                                                                    aaTATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCAtg                       <  2:61234/140‑1 (MQ=255)                                                                                                    aaTATGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCAtg                       <  2:704628/140‑1 (MQ=255)                                                                                                        tGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCa                         <  2:1449329/134‑1 (MQ=255)                                                                                                        tGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCa                         >  1:1449329/1‑134 (MQ=255)                                                                                                        tGCGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCAtgctgc                   <  1:225281/140‑1 (MQ=255)                                                                                                          cGTGGTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGctgctg                 >  1:583691/1‑140 (MQ=255)                                                                                                              gTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTg                            >  1:1138702/1‑125 (MQ=255)                                                                                                              gTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTg                            <  2:1138702/125‑1 (MQ=255)                                                                                                              gTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTa             >  1:1400073/1‑140 (MQ=255)                                                                                                              gTTCAACAACGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTa             >  1:1432498/1‑140 (MQ=255)                                                                                                                  aacaacGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAAt         <  1:1098097/140‑1 (MQ=255)                                                                                                                  aacaacGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAAt         <  2:938979/140‑1 (MQ=255)                                                                                                                   acaacGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATc        >  2:1478945/1‑140 (MQ=255)                                                                                                                   acaacGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATc        <  2:931691/140‑1 (MQ=255)                                                                                                                       cGTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCg    >  1:835316/1‑140 (MQ=255)                                                                                                                        gTGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGc   <  2:1586707/140‑1 (MQ=255)                                                                                                                         tGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCt  >  2:926489/1‑140 (MQ=255)                                                                                                                         tGGAAACCAAGTCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCt  >  2:499509/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                 GTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCT  >  NC_000913/1283614‑1283873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑