Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,365,831	C→T	A94A (GCC→GCT)	puuE →	4‑aminobutyrate aminotransferase PuuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,365,831	0	C	T	100.0%	245.8 / NA	71	A94A (GCC→GCT)	puuE	4‑aminobutyrate aminotransferase PuuE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/32);  total (39/32)

GCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCCCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCTTTCACGG  >  NC_000913/1365697‑1365965                                                                                                                                       |                                                                                                                                       gCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGc                                                                                                                                   >  2:872318/1‑140 (MQ=255)      gCTGAATACCGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCgg                                                                                                                              >  1:1386941/1‑140 (MQ=255)          aaTACCGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTAACCACACCGCGTATCAGATTGTGCCGGATGAAATCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAg                                                                                                                          >  2:1447568/1‑140 (MQ=255)            tACCGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCg                                                                                                                        >  1:672309/1‑140 (MQ=255)              ccGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCggg                                                                                                                      <  2:1361061/140‑1 (MQ=255)              ccGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCggg                                                                                                                      <  2:648059/140‑1 (MQ=255)               cGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGc                                                                                                                     >  1:358952/1‑140 (MQ=255)                 gACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCggg                                                                                                                      >  1:1466983/1‑137 (MQ=255)                 gACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCggg                                                                                                                      <  2:1466983/137‑1 (MQ=255)                     tCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCa                                                                                                               <  1:976618/140‑1 (MQ=255)                      cGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCaa                                                                                                              >  1:1038966/1‑140 (MQ=255)                      cGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCaa                                                                                                              >  2:381165/1‑140 (MQ=255)                          aCCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAAcc                                                                                                          <  1:1361515/140‑1 (MQ=255)                             cTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACcgcg                                                                                                       <  2:672309/140‑1 (MQ=255)                              tGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGt                                                                                                      >  1:1273974/1‑140 (MQ=255)                                aTCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGttc                                                                                                    <  2:358952/140‑1 (MQ=255)                                aTCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGttc                                                                                                    >  2:670970/1‑140 (MQ=255)                                aTCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGCGAGCGGGCAGGCCAAAACCGCGttc                                                                                                    >  1:926930/1‑140 (MQ=255)                                     gTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcac                                                                                               >  1:1477547/1‑140 (MQ=255)                                     gTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcac                                                                                               >  1:1039174/1‑140 (MQ=255)                                     gTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcac                                                                                               <  2:1211662/140‑1 (MQ=255)                                     gTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcac                                                                                               >  2:1198323/1‑140 (MQ=255)                                        gcggcgGTGGGGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcaccac                                                                                            >  1:1211411/1‑140 (MQ=255)                                        gcggcgGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcaccac                                                                                            <  2:1386941/140‑1 (MQ=255)                                        gcggcgGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcaccac                                                                                            <  2:764265/140‑1 (MQ=255)                                        gcggcgGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcaccac                                                                                            <  2:957144/140‑1 (MQ=255)                                        gcggcgGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTcaccac                                                                                            >  1:1463032/1‑140 (MQ=255)                                          ggcggTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCg                                                                                          >  1:210435/1‑140 (MQ=255)                                           gcggTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCgg                                                                                         <  2:210435/140‑1 (MQ=255)                                                   gcagcaACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAg                                                                                 <  1:989651/140‑1 (MQ=255)                                                         aCTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTgg                                                                           >  1:1154797/1‑140 (MQ=255)                                                               aCAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAAcg                                                                     <  2:1477547/140‑1 (MQ=255)                                                                        ccACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGaaaa                                                            <  1:872318/140‑1 (MQ=255)                                                                            acCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGc                                                        >  1:531039/1‑140 (MQ=255)                                                                             ccGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGCGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCt                                                       <  2:926930/140‑1 (MQ=255)                                                                               gcgTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcg                                                     >  2:618771/1‑140 (MQ=255)                                                                                cgTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcgc                                                    >  2:1141943/1‑140 (MQ=255)                                                                                cgTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcgc                                                    >  1:1363514/1‑140 (MQ=255)                                                                                cgTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcgc                                                    >  1:192086/1‑140 (MQ=255)                                                                                cgTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcgc                                                    >  1:304766/1‑140 (MQ=255)                                                                                 gTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcgcg                                                   <  2:192086/140‑1 (MQ=255)                                                                                 gTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTcgcg                                                   >  2:1324993/1‑140 (MQ=255)                                                                                  tATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCgcgc                                                  <  2:450969/140‑1 (MQ=255)                                                                                  tATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCgcgc                                                  <  1:618771/140‑1 (MQ=255)                                                                                      aGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCAt                                              >  2:1463215/1‑140 (MQ=255)                                                                                          tgtgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCg                                          >  1:920208/1‑140 (MQ=255)                                                                                          tgtgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCg                                          >  2:240862/1‑140 (MQ=255)                                                                                           gtgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCgg                                         <  1:1022403/140‑1 (MQ=255)                                                                                           gtgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCgg                                         <  2:1600089/140‑1 (MQ=255)                                                                                           gtgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCgg                                         <  2:1043468/140‑1 (MQ=255)                                                                                            tgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCt                                                       <  1:1510886/125‑1 (MQ=255)                                                                                            tgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCt                                                       >  2:1510886/1‑125 (MQ=255)                                                                                            tgCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGa                                        >  2:324793/1‑140 (MQ=255)                                                                                                gTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGcc                                    >  2:43290/1‑140 (MQ=255)                                                                                                 tATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGccc                                   <  1:43290/140‑1 (MQ=255)                                                                                                 tATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGccc                                   >  1:69974/1‑140 (MQ=255)                                                                                                      aaGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCgg                                         <  1:661034/129‑1 (MQ=255)                                                                                                      aaGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCgg                                         >  2:661034/1‑129 (MQ=255)                                                                                                      aaGCTACGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCg                              >  1:434386/1‑140 (MQ=255)                                                                                                           aCGTCACCCTGGTGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGAtt                         <  1:240862/140‑1 (MQ=255)                                                                                                           aCGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGAtt                         <  1:1198323/140‑1 (MQ=255)                                                                                                            cGTCACCCTGGCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTg                        >  1:125767/1‑140 (MQ=255)                                                                                                                     ggCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAgcg               <  2:1038966/140‑1 (MQ=255)                                                                                                                      gCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAgcgg              >  2:683151/1‑140 (MQ=255)                                                                                                                      gCGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAgcgg              >  2:502047/1‑140 (MQ=255)                                                                                                                       cGGAGAAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAgcggc             >  1:1273361/1‑140 (MQ=255)                                                                                                                           gaAAATCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCt         >  2:430810/1‑140 (MQ=255)                                                                                                                            aaaaTCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCtt        >  2:1246517/1‑140 (MQ=255)                                                                                                                               aTCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCTTTCa     <  1:381165/140‑1 (MQ=255)                                                                                                                               aTCAACGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCTTTCa     <  2:1463032/140‑1 (MQ=255)                                                                                                                                  aaCGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCTTTCACgg  <  2:125767/140‑1 (MQ=255)                                                                                                                                    cGCTCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCTTTc      <  1:99067/134‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTGCCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCCCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTTCACCACCGGTGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTAGCGGCGGCTTTCACGG  >  NC_000913/1365697‑1365965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑