Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,663,354	A→G	H122R (CAC→CGC)	ynfG →	putative oxidoreductase YnfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,663,354	0	A	G	100.0%	293.0 / NA	81	H122R (CAC→CGC)	ynfG	putative oxidoreductase YnfG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/35);  total (46/35)

TCTGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCACTCTTGCTGCC  >  NC_000913/1663222‑1663490                                                                                                                                     |                                                                                                                                         tCTGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATTGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACg                                                                                                                                   <  1:771944/140‑1 (MQ=255) tCTGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACg                                                                                                                                   <  2:922579/140‑1 (MQ=255) tCTGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACg                                                                                                                                   >  2:712079/1‑140 (MQ=255)   tGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACg                                                                                                                                   >  1:495010/1‑138 (MQ=255)   tGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACg                                                                                                                                   <  2:495010/138‑1 (MQ=255)   tGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGaa                                                                                                                                 >  2:361837/1‑140 (MQ=255)    gCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAg                                                                                                                                <  1:1007831/140‑1 (MQ=255)         aGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGa                                                                                                                           <  2:185648/140‑1 (MQ=255)         aGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGa                                                                                                                           <  1:358520/140‑1 (MQ=255)           cggcgCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGc                                                                                                                             >  2:1110384/1‑136 (MQ=255)           cggcgCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGc                                                                                                                             <  1:1110384/136‑1 (MQ=255)             gcgcAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGgt                                                                                                                       <  1:881182/140‑1 (MQ=255)             gcgcAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGgt                                                                                                                       >  1:807747/1‑140 (MQ=255)                          gCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTcgcgcg                                                                                                          <  1:712079/140‑1 (MQ=255)                             tGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCg                                                                                                       >  1:1422453/1‑140 (MQ=255)                             tGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCg                                                                                                       >  1:1193920/1‑140 (MQ=255)                               aaGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTcgcgc                                                                                                           <  1:1227116/134‑1 (MQ=255)                               aaGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTcgcgc                                                                                                           >  2:1227116/1‑134 (MQ=255)                               aaGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGcc                                                                                                     >  2:1344116/1‑140 (MQ=255)                                    ggCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAggg                                                                                                >  1:1591968/1‑140 (MQ=255)                                    ggCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAggg                                                                                                >  2:1348101/1‑140 (MQ=255)                                    ggCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAggg                                                                                                >  1:52126/1‑140 (MQ=255)                                        ttGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGaaa                                                                                            <  1:648038/140‑1 (MQ=255)                                        ttGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGaaa                                                                                            <  2:125995/140‑1 (MQ=255)                                         tgtgGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAaac                                                                                           >  2:935601/1‑140 (MQ=255)                                          gtggtgGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAaaca                                                                                          <  2:1548611/140‑1 (MQ=255)                                               ggtTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAaacaa                                                                                         >  2:734338/1‑136 (MQ=255)                                               ggtTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAaacaa                                                                                         <  1:734338/136‑1 (MQ=255)                                                gtTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGc                                                                                                      >  1:54867/1‑122 (MQ=255)                                                gtTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGc                                                                                                      <  2:54867/122‑1 (MQ=255)                                                     tgaAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATAtgtg                                                                               >  1:993398/1‑140 (MQ=255)                                                        aGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCg                                                                            >  2:326019/1‑140 (MQ=255)                                                          aTGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGaa                                                                          >  1:1505474/1‑140 (MQ=255)                                                           tGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAAt                                                                         >  2:1522965/1‑140 (MQ=255)                                                            gTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATc                                                                        >  1:1471570/1‑140 (MQ=255)                                                               tGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTg                                                                     >  1:205166/1‑140 (MQ=255)                                                               tGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTg                                                                     >  2:1332509/1‑140 (MQ=255)                                                                gTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGc                                                                    <  2:491728/140‑1 (MQ=255)                                                                   tCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCg                                                                 <  2:205166/140‑1 (MQ=255)                                                                   tCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCg                                                                 >  2:471708/1‑140 (MQ=255)                                                                      gCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTg                                                              >  1:1075192/1‑140 (MQ=255)                                                                      gCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTg                                                              >  1:1129297/1‑140 (MQ=255)                                                                        tGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCg                                                            <  1:361837/140‑1 (MQ=255)                                                                        tGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCg                                                            <  1:935601/140‑1 (MQ=255)                                                                         gCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGt                                                           <  1:471708/140‑1 (MQ=255)                                                                                 tGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCg                                                            >  2:386529/1‑131 (MQ=255)                                                                                 tGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCg                                                            <  1:386529/131‑1 (MQ=255)                                                                                  gCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAg                                                  <  1:1344116/140‑1 (MQ=255)                                                                                    cacaTGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGtt                                                <  2:1075192/140‑1 (MQ=255)                                                                                       aTGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCgg                                             <  1:326019/140‑1 (MQ=255)                                                                                        tGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGt                                            >  1:109039/1‑140 (MQ=255)                                                                                        tGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGt                                            >  2:62195/1‑140 (MQ=255)                                                                                         ggCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTc                                           <  2:807747/140‑1 (MQ=255)                                                                                          gCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTcc                                          <  1:1334198/140‑1 (MQ=255)                                                                                          gCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTcc                                          >  2:1348939/1‑140 (MQ=255)                                                                                           cTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCa                                         >  2:423374/1‑140 (MQ=255)                                                                                           cTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCa                                         <  2:1193920/140‑1 (MQ=255)                                                                                               tCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGtt                                                >  1:1584953/1‑129 (MQ=255)                                                                                               tCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGtt                                                <  2:1584953/129‑1 (MQ=255)                                                                                               tCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTg                                     >  1:471121/1‑140 (MQ=255)                                                                                                   tACGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAaga                                 >  2:10649/1‑140 (MQ=255)                                                                                                    aCGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAagag                                <  2:471121/140‑1 (MQ=255)                                                                                                     cGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGc                               <  1:10649/140‑1 (MQ=255)                                                                                                     cGGCGCGCCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGc                               <  1:1332509/140‑1 (MQ=255)                                                                                                         gcgcCACAGTACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAGACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTgcg                           >  2:1393014/1‑140 (MQ=255)                                                                                                                  tACAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCa                  >  2:504645/1‑140 (MQ=255)                                                                                                                    cAATGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACg                <  1:1522965/140‑1 (MQ=255)                                                                                                                      aTGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGc              <  2:52126/140‑1 (MQ=255)                                                                                                                       tGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCa             <  2:1505474/140‑1 (MQ=255)                                                                                                                       tGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCa             >  1:1205745/1‑140 (MQ=255)                                                                                                                       tGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCa             >  2:552893/1‑140 (MQ=255)                                                                                                                       tGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCa             >  2:1587245/1‑140 (MQ=255)                                                                                                                       tGCTGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCAATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCa             >  1:1082271/1‑140 (MQ=255)                                                                                                                          tGAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCActc          >  1:570562/1‑140 (MQ=255)                                                                                                                           gAAAAAGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCActct         <  1:1587245/140‑1 (MQ=255)                                                                                                                            aaaaaGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTTCAATTGAAGAGCTGCGCCAGAAGCACGGCACTCtt        >  2:1111397/1‑140 (MQ=255)                                                                                                                            aaaaaGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCACTCtt        >  1:489416/1‑140 (MQ=255)                                                                                                                            aaaaaGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCACTCtt        >  2:1369280/1‑140 (MQ=255)                                                                                                                            aaaaaGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCACTCtt        >  2:243967/1‑140 (MQ=255)                                                                                                                            aaaaaGGGCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTACAATTGAAGAGCTGCGCCAGAAGCACGGCACTCtt        >  2:1107084/1‑140 (MQ=255)                                                                                                                               aaGGGCGTATGACGAAGTGCGATGGTTGTTATTCGCGCGGCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTATAATTGAAGAGCTGCGCCAGAAGCACGGCACTCTtgct     <  2:1422453/140‑1 (MQ=255)                                                                                                                                 gggCGCATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAG‑GCTGCGCCAGAAGCACGGCACTCTTGCTGcc  >  1:708628/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         TCTGCCCCAGCGGCGCAATGCATAAGCGTGAAGATGGCTTTGTGGTGGTTGATGAAGATGTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGCTGCGTGCACTTGAGTTCGGTCCAATTGAAGAGCTGCGCCAGAAGCACGGCACTCTTGCTGCC  >  NC_000913/1663222‑1663490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑