Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,690	A→G	G362G (GGT→GGC)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,690	0	A	G	100.0%	327.0 / NA	90	G362G (GGT→GGC)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/37);  total (53/37)

CGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGC  >  NC_000913/217556‑217822                                                                                                                                       |                                                                                                                                     cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCcagc                                                                                                                                 >  2:1370257/1‑140 (MQ=255) cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCcagc                                                                                                                                 >  2:1074888/1‑140 (MQ=255)   tttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCcagcag                                                                                                                               <  1:269244/140‑1 (MQ=255)   tttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCcagcag                                                                                                                               <  2:426052/140‑1 (MQ=255)            cagcCTACCCTGGCCATCCGGGCTTGGATCGCCTGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCAc                                                                                                                      >  2:1267913/1‑140 (MQ=255)            cagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCAc                                                                                                                      >  2:837666/1‑140 (MQ=255)            cagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCAc                                                                                                                      >  2:717321/1‑140 (MQ=255)            cagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCAc                                                                                                                      >  1:1332913/1‑140 (MQ=255)             agcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACt                                                                                                                     <  2:1302236/140‑1 (MQ=255)                cTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCAt                                                                                                                  <  1:1561719/140‑1 (MQ=255)                 tACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATc                                                                                                                 <  2:165132/140‑1 (MQ=255)                      tGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATcgccgc                                                                                                            <  1:717321/140‑1 (MQ=255)                      tGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATcgccg                                                                                                             >  2:684475/1‑139 (MQ=255)                      tGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATcgccg                                                                                                             <  1:684475/139‑1 (MQ=255)                        gCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCaa                                                                                                          >  2:300580/1‑140 (MQ=255)                            tCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggt                                                                                                      <  2:1332913/140‑1 (MQ=255)                             ccGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggta                                                                                                     >  1:1013929/1‑140 (MQ=255)                             ccGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggta                                                                                                     >  1:840098/1‑140 (MQ=255)                             ccGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggta                                                                                                     >  1:836501/1‑140 (MQ=255)                             ccGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggta                                                                                                     <  1:744419/140‑1 (MQ=255)                             ccGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggta                                                                                                     >  2:1554486/1‑140 (MQ=255)                             ccGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggta                                                                                                     >  1:1001887/1‑140 (MQ=255)                              cGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAacggtac                                                                                                    <  1:1074888/140‑1 (MQ=255)                                   ttGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCa                                                                                               <  1:377710/140‑1 (MQ=255)                                     ggATCGCCTGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt                                                                                             <  1:1267913/140‑1 (MQ=255)                                      gATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATc                                                                                            >  1:600678/1‑140 (MQ=255)                                       aTCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATca                                                                                           >  2:700743/1‑140 (MQ=255)                                          gccagccaCCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca                                                                                        >  2:314805/1‑140 (MQ=255)                                          gccagccaCCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca                                                                                        >  1:1282467/1‑140 (MQ=255)                                           ccagccaCCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcaccac                                                                                       >  2:867243/1‑140 (MQ=255)                                           ccagccaCCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcaccac                                                                                       >  2:891978/1‑140 (MQ=255)                                               ccaccaCGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGa                                                                                   >  1:1469671/1‑140 (MQ=255)                                                 accacGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAAt                                                                                 >  1:1196695/1‑140 (MQ=255)                                                    acGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCgg                                                                              <  2:1013929/140‑1 (MQ=255)                                                     cGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGc                                                                             <  2:177792/140‑1 (MQ=255)                                                       ttACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCAt                                                                           <  1:891978/140‑1 (MQ=255)                                                         aCGGATATATGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGt                                                                         >  2:1317717/1‑140 (MQ=255)                                                             atatCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTAc                                                                     >  2:323260/1‑140 (MQ=255)                                                             atatCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTAc                                                                     <  1:837666/140‑1 (MQ=255)                                                             atatCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTAc                                                                     >  1:357488/1‑140 (MQ=255)                                                              tatCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTAcc                                                                    <  2:836501/140‑1 (MQ=255)                                                               atCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCg                                                                   >  1:55422/1‑140 (MQ=255)                                                               atCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCg                                                                   <  2:1090377/140‑1 (MQ=255)                                                               atCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCg                                                                   >  1:257722/1‑140 (MQ=255)                                                                tCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCgg                                                                  >  1:376714/1‑140 (MQ=255)                                                                tCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCgg                                                                  >  2:420580/1‑140 (MQ=255)                                                                 cTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGa                                                                 <  2:376714/140‑1 (MQ=255)                                                                  tGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGAc                                                                >  1:1189832/1‑140 (MQ=255)                                                                    cAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGAccc                                                              >  1:399164/1‑140 (MQ=255)                                                                    cAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGAccc                                                              >  2:1573225/1‑140 (MQ=255)                                                                      aCTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAg                                                            <  2:55422/140‑1 (MQ=255)                                                                         tCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGa                                                         >  2:822066/1‑140 (MQ=255)                                                                          ccGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGaa                                                        >  2:63526/1‑140 (MQ=255)                                                                          ccGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGaa                                                        >  1:1164832/1‑140 (MQ=255)                                                                          ccGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGaa                                                        >  1:354063/1‑140 (MQ=255)                                                                                tAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGa                                                  <  1:1370257/140‑1 (MQ=255)                                                                                tAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGa                                                  <  1:926379/140‑1 (MQ=255)                                                                                 aGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGAc                                                 <  2:600678/140‑1 (MQ=255)                                                                                 aGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGAc                                                 <  2:1178118/140‑1 (MQ=255)                                                                                 aGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGAc                                                 <  1:420580/140‑1 (MQ=255)                                                                                 aGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGAc                                                 <  1:1573225/140‑1 (MQ=255)                                                                                  gCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGa                                                                 >  2:556519/1‑123 (MQ=255)                                                                                  gCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGa                                                                 <  1:556519/123‑1 (MQ=255)                                                                                  gCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGa                                                  >  1:247064/1‑138 (MQ=255)                                                                                  gCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGa                                                  <  2:247064/138‑1 (MQ=255)                                                                                   cGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCg                                               >  2:1348308/1‑140 (MQ=255)                                                                                     aCATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGc                                             <  2:1469671/140‑1 (MQ=255)                                                                                         cgcgATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTa                                         >  2:277646/1‑140 (MQ=255)                                                                                            gATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAAcc                                      <  1:277646/140‑1 (MQ=255)                                                                                                        tGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAtt                          >  2:124335/1‑140 (MQ=255)                                                                                                        tGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAtt                          >  1:1041161/1‑140 (MQ=255)                                                                                                        tGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAtt                          <  1:1554486/140‑1 (MQ=255)                                                                                                        tGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAtt                          >  1:1491780/1‑140 (MQ=255)                                                                                                        tGCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAtt                          >  2:1560109/1‑140 (MQ=255)                                                                                                         gCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAGCGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAttt                         >  1:1563295/1‑140 (MQ=255)                                                                                                         gCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAttt                         >  1:552530/1‑140 (MQ=255)                                                                                                         gCCGAAGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAAttt                         >  1:1456900/1‑140 (MQ=255)                                                                                                             aaGTAGTGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATttcttc                     >  1:1104579/1‑140 (MQ=255)                                                                                                                tagtGTTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATttcttcttc                  <  2:1491780/140‑1 (MQ=255)                                                                                                                  gtgtTTACCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCgg                <  1:124335/140‑1 (MQ=255)                                                                                                                        aCCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGa          >  1:1522072/1‑140 (MQ=255)                                                                                                                        aCCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGa          >  1:1304321/1‑140 (MQ=255)                                                                                                                        aCCATCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGa          <  1:63526/140‑1 (MQ=255)                                                                                                                            tCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGt      <  2:357488/140‑1 (MQ=255)                                                                                                                            tCGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGt      >  1:652312/1‑140 (MQ=255)                                                                                                                            tCGATGTTAGCGCCAGCAGCAAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGt      >  2:227855/1‑140 (MQ=255)                                                                                                                             cGATGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTc     >  1:1525629/1‑140 (MQ=255)                                                                                                                               aTGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAg   <  2:1053343/140‑1 (MQ=255)                                                                                                                               aTGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAg   >  1:1450364/1‑140 (MQ=255)                                                                                                                               aTGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAg   >  1:1531797/1‑140 (MQ=255)                                                                                                                                tGTTAGCGCCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGc  <  2:840098/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGC  >  NC_000913/217556‑217822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑