Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,424	T→C	V483A (GTT→GCT)	fadK →	short chain acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,424	0	T	C	100.0%	313.3 / NA	86	V483A (GTT→GCT)	fadK	short chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/45);  total (41/45)

GTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGA  >  NC_000913/1784289‑1784559                                                                                                                                        |                                                                                                                                        gTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCa                                                                                                                                     <  2:1065066/140‑1 (MQ=255)   aTGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAAt                                                                                                                                   >  2:379601/1‑140 (MQ=255)      gATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTc                                                                                                                                <  1:1602785/140‑1 (MQ=255)       aTGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTcc                                                                                                                               >  2:979205/1‑140 (MQ=255)       aTGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTcc                                                                                                                               >  2:1333157/1‑140 (MQ=255)          aGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGAt                                                                                                                            <  1:1234385/140‑1 (MQ=255)          aGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGAt                                                                                                                            <  2:959263/140‑1 (MQ=255)            gctggctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGa                                                                                                                          >  2:611918/1‑140 (MQ=255)             ctggctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGaa                                                                                                                         >  2:1184763/1‑140 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGaa                                                                                                                         >  1:1211272/1‑137 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGaa                                                                                                                         <  2:1211272/137‑1 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGt                                                                                                                      >  1:1007377/1‑140 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGt                                                                                                                      >  2:1177360/1‑140 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGt                                                                                                                      >  1:1254822/1‑140 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGt                                                                                                                      >  2:929373/1‑140 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGt                                                                                                                      >  2:1458836/1‑140 (MQ=255)                gctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGt                                                                                                                      >  1:1567934/1‑140 (MQ=255)                 ctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGtt                                                                                                                     <  1:1184763/140‑1 (MQ=255)                  tatataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGttt                                                                                                                    <  1:379601/140‑1 (MQ=255)                  tatataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGttt                                                                                                                    <  1:282858/140‑1 (MQ=255)                  tatataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGttt                                                                                                                    <  2:1567934/140‑1 (MQ=255)                     ataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAgg                                                                                                                 >  2:1349292/1‑140 (MQ=255)                          aaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGtt                                                                                                                     >  2:1109216/1‑131 (MQ=255)                          aaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGtt                                                                                                                     <  1:1109216/131‑1 (MQ=255)                          aaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAAc                                                                                                            >  1:1164102/1‑140 (MQ=255)                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTg                                                                                                               >  2:281049/1‑133 (MQ=255)                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTg                                                                                                               <  1:281049/133‑1 (MQ=255)                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                        >  2:222947/1‑140 (MQ=255)                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                        <  2:585020/140‑1 (MQ=255)                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                        >  1:286515/1‑140 (MQ=255)                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                        >  1:355245/1‑140 (MQ=255)                              aCCGGACGC‑AAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                       <  1:836081/140‑1 (MQ=255)                               ccGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                       <  2:574485/140‑1 (MQ=255)                                cGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCAt                                                                                                      <  1:884341/140‑1 (MQ=255)                                          aaaGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGt                                                                                            <  1:507690/140‑1 (MQ=255)                                             gATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGt                                                                                         <  1:929373/140‑1 (MQ=255)                                               tattatTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGc                                                                                       <  1:222947/140‑1 (MQ=255)                                                  tattGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGa                                                                                    <  1:1349292/140‑1 (MQ=255)                                                   attGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATg                                                                                             >  1:589988/1‑130 (MQ=255)                                                   attGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATg                                                                                             <  2:589988/130‑1 (MQ=255)                                                   attGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGaa                                                                                   >  1:471308/1‑140 (MQ=255)                                                       tCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                               <  1:611918/140‑1 (MQ=255)                                                       tCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                               <  1:324242/140‑1 (MQ=255)                                                       tCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                               >  2:909270/1‑140 (MQ=255)                                                       tCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                               <  1:1064794/140‑1 (MQ=255)                                                        ccGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcgc                                                                              <  2:286515/140‑1 (MQ=255)                                                        ccGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                               >  2:901925/1‑139 (MQ=255)                                                        ccGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                               <  1:901925/139‑1 (MQ=255)                                                         cgcgGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGa                                                                                    <  2:659579/133‑1 (MQ=255)                                                         cgcgGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGa                                                                                    >  1:659579/1‑133 (MQ=255)                                                         cgcgGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgcc                                                                             >  2:191685/1‑140 (MQ=255)                                                           cggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgccgc                                                                           >  2:1012043/1‑140 (MQ=255)                                                           cggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgccgc                                                                           >  2:124926/1‑140 (MQ=255)                                                            ggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGca                                                                          >  1:1106493/1‑140 (MQ=255)                                                            ggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGACGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGca                                                                          <  1:1303725/140‑1 (MQ=255)                                                                gcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGcatcat                                                                      >  2:843266/1‑140 (MQ=255)                                                                 cgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcatt                                                                     <  2:753338/140‑1 (MQ=255)                                                                        atTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATc                                                              <  2:541900/140‑1 (MQ=255)                                                                        atTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATc                                                              <  2:1254822/140‑1 (MQ=255)                                                                                 cGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAaga                                                     >  1:1210321/1‑140 (MQ=255)                                                                                          gAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGc                                            >  2:284493/1‑140 (MQ=255)                                                                                                atTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCtttttt                                      <  2:355245/140‑1 (MQ=255)                                                                                                atTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCtttttt                                      >  2:1544283/1‑140 (MQ=255)                                                                                                atTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCtttttt                                      <  2:1007377/140‑1 (MQ=255)                                                                                                         cagcaTCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTaa                             >  1:1378566/1‑140 (MQ=255)                                                                                                           gcaTCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTa                                    <  2:1527494/131‑1 (MQ=255)                                                                                                           gcaTCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTa                                    >  1:1527494/1‑131 (MQ=255)                                                                                                           gcaTCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAAc                           >  1:511773/1‑140 (MQ=255)                                                                                                            caTCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACg                          >  2:1128648/1‑140 (MQ=255)                                                                                                              tCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACggg                        <  1:1012043/140‑1 (MQ=255)                                                                                                              tCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACggg                        >  2:647262/1‑140 (MQ=255)                                                                                                              tCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACggg                        >  2:1148069/1‑140 (MQ=255)                                                                                                              tCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACggg                        >  1:1534927/1‑140 (MQ=255)                                                                                                                  aaaaTTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGc                    <  2:1164102/140‑1 (MQ=255)                                                                                                                  aaaaTTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGc                    <  2:471308/140‑1 (MQ=255)                                                                                                                      ttCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaaaa                <  2:851091/140‑1 (MQ=255)                                                                                                                           gATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataa           <  1:284493/140‑1 (MQ=255)                                                                                                                           gATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataa           <  1:1089422/140‑1 (MQ=255)                                                                                                                             tGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataaat         <  1:1177360/140‑1 (MQ=255)                                                                                                                             tGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataaat         >  1:865373/1‑140 (MQ=255)                                                                                                                                cTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATc      <  1:191685/140‑1 (MQ=255)                                                                                                                                cTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATc      >  1:1246557/1‑140 (MQ=255)                                                                                                                                 tgtgtgGCTGCAATGTCCGATGAACGTTTAGGTGATCGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATcc     <  2:1106493/140‑1 (MQ=255)                                                                                                                                 tgtgtgGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATcc     <  1:1458836/140‑1 (MQ=255)                                                                                                                                  gtgtgGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCt    <  1:124926/140‑1 (MQ=255)                                                                                                                                    gtgGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGa  <  1:1544283/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGA  >  NC_000913/1784289‑1784559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑