Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,395	G→A	G196G (GGC→GGT)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,395	0	G	A	100.0%	123.4 / NA	39	G196G (GGC→GGT)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/31);  total (8/31)

TGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGC  >  NC_000913/1831264‑1831530                                                                                                                                    |                                                                                                                                        tGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTcccc                                                                                                                                 <  2:539381/140‑1 (MQ=255)     tataGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTgg                                                                                                                             <  1:596494/140‑1 (MQ=255)      ataGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACAACCTTCCCCCTGGa                                                                                                                            <  2:786559/140‑1 (MQ=255)       taGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGAt                                                                                                                           <  1:160156/140‑1 (MQ=255)           cATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGt                                                                                                                       <  1:186454/140‑1 (MQ=255)            aTACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGtt                                                                                                                      <  1:5657/140‑1 (MQ=255)                   tCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAGCGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAAt                                                                                                               <  2:185831/140‑1 (MQ=255)                   tCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAAt                                                                                                               <  1:110448/140‑1 (MQ=255)                      ccGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCAc                                                                                                            <  2:92312/140‑1 (MQ=255)                        ggTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCg                                                                                                          <  2:577137/140‑1 (MQ=255)                         gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGc                                                                                                         <  2:56857/140‑1 (MQ=255)                          tGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGca                                                                                                        <  1:1566066/140‑1 (MQ=255)                                cGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTa                                                                                                  <  1:184844/140‑1 (MQ=255)                                 gACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTag                                                                                                 <  2:1545857/140‑1 (MQ=255)                                                ttCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAgcg                                                                                  <  2:1074899/140‑1 (MQ=255)                                                      aaaaaTCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTgg                                                                            <  2:80588/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGTGCGCTGGCAGAGTTAata                                                                 <  1:1082224/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                 <  1:1191117/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                 <  2:910757/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                 <  2:1178888/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                 <  1:987324/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                 <  2:1038365/140‑1 (MQ=255)                                                                 gcgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                 <  1:22737/140‑1 (MQ=255)                                                                                tACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATgcagca                                                  <  1:678390/140‑1 (MQ=255)                                                                                         cACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAata                                         >  1:1154858/1‑140 (MQ=255)                                                                                               gACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATccgcc                                   >  1:1047262/1‑140 (MQ=255)                                                                                                 ccTTGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCgg                                 >  2:222534/1‑140 (MQ=255)                                                                                                    tGTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAg                              <  1:1381984/140‑1 (MQ=255)                                                                                                     gTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCGCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGaa                                           >  2:1109331/1‑126 (MQ=255)                                                                                                     gTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGaa                                           <  1:1109331/126‑1 (MQ=255)                                                                                                     gTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGaa                                           >  2:1417840/1‑126 (MQ=255)                                                                                                     gTAAAAACGCGTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGaa                                           <  1:1417840/126‑1 (MQ=255)                                                                                                            cgcgTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCaa                      >  2:772063/1‑140 (MQ=255)                                                                                                            cgcgTTGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCaa                      >  1:529120/1‑140 (MQ=255)                                                                                                                ttGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATc                  >  2:122812/1‑140 (MQ=255)                                                                                                                 tGCTGGCTGGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATcc                 <  1:122812/140‑1 (MQ=255)                                                                                                                  gctggcttGCACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCt                <  1:449140/140‑1 (MQ=255)                                                                                                                       ctggcACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGaa           <  1:772063/140‑1 (MQ=255)                                                                                                                         ggcACCACACCACCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATa         <  2:1154858/140‑1 (MQ=255)                                                                                                                           caccacaccaCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAgg       >  1:1138954/1‑140 (MQ=255)                                                                                                                               ccacCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATAGGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctgg   <  2:1168359/139‑1 (MQ=255)                                                                                                                                caccacCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctggc  >  1:388599/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGC  >  NC_000913/1831264‑1831530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑