Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,902,770	G→A	M241I (ATG→ATA)	manX →	mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,902,770	0	G	A	100.0%	283.2 / NA	80	M241I (ATG→ATA)	manX	mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/40);  total (40/40)

TGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAACGCGGTTTCGGTTG  >  NC_000913/1902635‑1902897                                                                                                                                        |                                                                                                                                tgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGt                                                                                                                             >  2:638708/1‑140 (MQ=255) tgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGt                                                                                                                             <  2:625254/140‑1 (MQ=255)        gCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAc                                                                                                                      >  1:652860/1‑140 (MQ=255)        gCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAc                                                                                                                      >  1:544693/1‑140 (MQ=255)         cTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAcc                                                                                                                     <  1:114327/140‑1 (MQ=255)         cTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAcc                                                                                                                     <  1:520237/140‑1 (MQ=255)          tGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAcca                                                                                                                    >  2:984746/1‑140 (MQ=255)          tGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAcca                                                                                                                    >  2:1489312/1‑140 (MQ=255)           gCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaa                                                                                                                   <  1:1481886/140‑1 (MQ=255)              gATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaaccc                                                                                                                <  2:1281171/140‑1 (MQ=255)              gATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaaccc                                                                                                                <  1:1114691/140‑1 (MQ=255)                tACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaacccaa                                                                                                              <  2:288017/140‑1 (MQ=255)                tACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaacccaa                                                                                                              <  2:382635/140‑1 (MQ=255)                tACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaacccaa                                                                                                              <  2:1554394/140‑1 (MQ=255)                tACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaacccaa                                                                                                              <  1:18662/140‑1 (MQ=255)                tACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaacccaa                                                                                                              <  2:1158006/140‑1 (MQ=255)                 aCCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTAccaacccaac                                                                                                             <  2:544693/140‑1 (MQ=255)                   cGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAg                                                                                                           <  2:1087653/140‑1 (MQ=255)                   cGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAg                                                                                                           >  2:403744/1‑140 (MQ=255)                     ttCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGAt                                                                                                         >  2:481523/1‑140 (MQ=255)                      tCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATg                                                                                                        >  1:1578681/1‑140 (MQ=255)                      tCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATg                                                                                                        >  2:1134321/1‑140 (MQ=255)                               acTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGtc                                                                                               <  2:556931/140‑1 (MQ=255)                               acTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGtc                                                                                               <  2:1525910/140‑1 (MQ=255)                                 tGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGtctc                                                                                             >  1:974768/1‑140 (MQ=255)                                  gCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACGACCCTAAATATGCTGGCGAACGCGTAATACTGTTATTTACTAACCCAACAGATGTAGAGCGTCTCg                                                                                            >  1:980495/1‑140 (MQ=255)                                           ggTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTaga                                                                                                    >  2:129673/1‑123 (MQ=255)                                           ggTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTaga                                                                                                    <  1:129673/123‑1 (MQ=255)                                           ggTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAggcg                                                                                   >  1:1431573/1‑140 (MQ=255)                                             ttGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAggcggc                                                                                 >  2:995504/1‑140 (MQ=255)                                              tGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGgcggcg                                                                                <  2:164060/140‑1 (MQ=255)                                               gCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGt                                                                               >  2:127478/1‑140 (MQ=255)                                               gCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGt                                                                               >  1:997225/1‑140 (MQ=255)                                                cACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTg                                                                              >  2:944521/1‑140 (MQ=255)                                                     ccGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAAt                                                                         <  1:523406/140‑1 (MQ=255)                                                     ccGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAAt                                                                         <  2:652860/140‑1 (MQ=255)                                                         gCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCAcc                                                                     <  1:858326/140‑1 (MQ=255)                                                                   acacGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACg                                                           >  1:202293/1‑140 (MQ=255)                                                                    cacGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGt                                                          <  2:1578681/140‑1 (MQ=255)                                                                     acGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTc                                                         <  1:817333/140‑1 (MQ=255)                                                                       gTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCgg                                                       <  1:290934/140‑1 (MQ=255)                                                                       gTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCgg                                                       >  1:1443837/1‑140 (MQ=255)                                                                       gTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCgg                                                       <  1:481523/140‑1 (MQ=255)                                                                          gTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCggtgg                                                    >  2:244521/1‑140 (MQ=255)                                                                            tGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTa                                                  >  1:904976/1‑140 (MQ=255)                                                                             gATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTAt                                                 <  1:944521/140‑1 (MQ=255)                                                                             gATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTAt                                                 >  1:852546/1‑140 (MQ=255)                                                                             gATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATG‑AGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATg                                                >  2:1096472/1‑140 (MQ=255)                                                                              aTGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATg                                                >  1:794913/1‑140 (MQ=255)                                                                               tGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATgg                                               >  2:373865/1‑140 (MQ=255)                                                                                 ttGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCa                                             >  2:834718/1‑140 (MQ=255)                                                                                   gCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCAtt                                           >  2:1344294/1‑140 (MQ=255)                                                                                   gCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCAtt                                           >  2:1190650/1‑140 (MQ=255)                                                                                   gCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCAtt                                           >  1:1287860/1‑140 (MQ=255)                                                                                   gCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCAtt                                           >  1:899303/1‑140 (MQ=255)                                                                                     cAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTcc                                         >  1:428270/1‑140 (MQ=255)                                                                                      aaaaTGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCg                                        >  2:1415888/1‑140 (MQ=255)                                                                                        aaTGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTc                                      <  1:1489312/140‑1 (MQ=255)                                                                                         aTGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCa                                     <  2:1443837/140‑1 (MQ=255)                                                                                            aTTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAggg                                  <  2:428270/140‑1 (MQ=255)                                                                                             ttCGCGTCTACAACAACCCGAAATATGCTGCCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGt                                 <  2:983154/140‑1 (MQ=255)                                                                                              tCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTa                                <  1:1115698/140‑1 (MQ=255)                                                                                              tCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTa                                >  2:382021/1‑140 (MQ=255)                                                                                              tCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTa                                <  2:904976/140‑1 (MQ=255)                                                                                              tCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTa                                <  2:852546/140‑1 (MQ=255)                                                                                                 cgTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTaaaa                             <  1:127478/140‑1 (MQ=255)                                                                                                   tCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAAcc                           <  1:718758/140‑1 (MQ=255)                                                                                                   tCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAAcc                           >  1:722409/1‑140 (MQ=255)                                                                                                   tCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATGCCGTCAGGGTAAAAcc                           <  1:59828/140‑1 (MQ=255)                                                                                                   tCTACAACAACCCGAAATATGCTGGCGAACGCGTAATACTGTTATTCACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAAcc                           <  2:1589907/140‑1 (MQ=255)                                                                                                          caacCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGa                    >  2:1156895/1‑140 (MQ=255)                                                                                                           aacCCGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGCAAAACCCAGGTGaa                   <  2:597056/140‑1 (MQ=255)                                                                                                           aacCCGAAATATGCTGGCGAACCCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGaa                   <  1:1134321/140‑1 (MQ=255)                                                                                                               cGAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAc               >  2:59231/1‑140 (MQ=255)                                                                                                                gAAATATGCTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAcg              >  1:131103/1‑140 (MQ=255)                                                                                                                        cTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAcg              >  2:698225/1‑132 (MQ=255)                                                                                                                        cTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAcg              <  1:698225/132‑1 (MQ=255)                                                                                                                        cTGGCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAACGCGGTTTCg      >  1:406687/1‑140 (MQ=255)                                                                                                                           gCGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAACGCGGTTTCGGtt   <  1:403744/140‑1 (MQ=255)                                                                                                                            cGAACGCGTAATACTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAACGCGGTTTCGGTtg  >  1:799305/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                TGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAACGCGGTTTCGGTTG  >  NC_000913/1902635‑1902897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑