Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,880	C→T	intergenic (‑105/+7)	proS ← / ← trmO	proline‑‑tRNA ligase/tRNA m(6)t(6)A37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,880	0	C	T	100.0%	246.5 / NA	74	intergenic (‑105/+7)	proS/trmO	proline‑‑tRNA ligase/tRNA m(6)t(6)A37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/33);  total (41/33)

GAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCC  >  NC_000913/218743‑219016                                                                                                                                          |                                                                                                                                         gagaGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGgtgt                                                                                                                                        >  1:1328900/1‑140 (MQ=255) gagaGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGgtgt                                                                                                                                        >  1:402433/1‑140 (MQ=255)      tGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAAtt                                                                                                                                   >  2:799896/1‑140 (MQ=255)           agCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAAcg                                                                                                                              >  2:287488/1‑140 (MQ=255)            gCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAAcgc                                                                                                                             >  1:278704/1‑140 (MQ=255)                  aTTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAAcgc                                                                                                                             <  2:1514483/134‑1 (MQ=255)                  aTTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAAcgc                                                                                                                             >  1:1514483/1‑134 (MQ=255)                        tAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCa                                                                                                                 <  1:1370833/140‑1 (MQ=255)                          gTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGtt                                                                                                                         <  2:1551046/130‑1 (MQ=255)                          gTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGtt                                                                                                                         >  1:1551046/1‑130 (MQ=255)                          gTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCaaa                                                                                                               >  1:359191/1‑140 (MQ=255)                          gTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCaaa                                                                                                               >  2:1587486/1‑140 (MQ=255)                          gTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCaaa                                                                                                               >  1:1484227/1‑140 (MQ=255)                           tACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGTAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAg                                                                                                              <  1:1587486/140‑1 (MQ=255)                           tACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAg                                                                                                              >  1:763185/1‑140 (MQ=255)                           tACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAg                                                                                                              <  1:573553/140‑1 (MQ=255)                               cATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACtt                                                                                                          <  2:575666/140‑1 (MQ=255)                                   ttgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTcc                                                                                                                       <  2:675047/123‑1 (MQ=255)                                   ttgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTcc                                                                                                                       >  1:675047/1‑123 (MQ=255)                                     gttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAAc                                                                                                    >  2:1595394/1‑140 (MQ=255)                                      ttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAAcc                                                                                                   <  2:278704/140‑1 (MQ=255)                                            ttCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTc                                                                                             >  1:305365/1‑140 (MQ=255)                                            ttCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTc                                                                                             >  2:399784/1‑140 (MQ=255)                                              ccAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCgg                                                                                           >  2:1069922/1‑140 (MQ=255)                                               cAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGt                                                                                          <  2:1328900/140‑1 (MQ=255)                                               cAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGt                                                                                          >  2:572635/1‑140 (MQ=255)                                                aGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTg                                                                                         <  1:287488/140‑1 (MQ=255)                                                      aaGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACgcgc                                                                                   >  1:306505/1‑140 (MQ=255)                                                      aaGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACgcgc                                                                                   >  1:1125237/1‑140 (MQ=255)                                                          tAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGc                                                                               >  2:1019363/1‑140 (MQ=255)                                                           aGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCg                                                                              <  2:305365/140‑1 (MQ=255)                                                              aCAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAAc                                                                           >  2:1185288/1‑140 (MQ=255)                                                                  gCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTa                                                                       >  1:690077/1‑140 (MQ=255)                                                                  gCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTa                                                                       >  2:1091520/1‑140 (MQ=255)                                                                   cTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaa                                                                      <  1:1595394/140‑1 (MQ=255)                                                                    tCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     >  1:387025/1‑140 (MQ=255)                                                                    tCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     <  1:138245/140‑1 (MQ=255)                                                                    tCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     <  2:1272073/140‑1 (MQ=255)                                                                       aGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     >  2:1287450/1‑137 (MQ=255)                                                                       aGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     >  2:1584153/1‑137 (MQ=255)                                                                       aGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     <  1:1287450/137‑1 (MQ=255)                                                                       aGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                                                                     <  1:1584153/137‑1 (MQ=255)                                                                            aGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTa                                                             >  1:1170864/1‑140 (MQ=255)                                                                            aGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTa                                                             >  2:1160758/1‑140 (MQ=255)                                                                              ccTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGc                                                           >  2:1588991/1‑140 (MQ=255)                                                                                tGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa                                                         <  2:690077/140‑1 (MQ=255)                                                                                tGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa                                                         <  1:1069922/140‑1 (MQ=255)                                                                                tGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa                                                         <  1:399784/140‑1 (MQ=255)                                                                                 gggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAg                                                        <  1:1019363/140‑1 (MQ=255)                                                                                       aaaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCa                                                  <  1:1091520/140‑1 (MQ=255)                                                                                        aaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAg                                                            <  1:1573287/129‑1 (MQ=255)                                                                                        aaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAg                                                            >  2:1573287/1‑129 (MQ=255)                                                                                                  tttAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc                                       >  2:298856/1‑140 (MQ=255)                                                                                                         tACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTcc                                <  2:1170864/140‑1 (MQ=255)                                                                                                          aCCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCt                               <  1:1588991/140‑1 (MQ=255)                                                                                                          aCCAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCt                               <  2:359191/140‑1 (MQ=255)                                                                                                           ccAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                              >  1:227043/1‑140 (MQ=255)                                                                                                           ccAGTGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                              <  2:227043/140‑1 (MQ=255)                                                                                                               tGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAGAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCt                          >  2:1430558/1‑140 (MQ=255)                                                                                                               tGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGccc                                      >  1:426812/1‑128 (MQ=255)                                                                                                               tGCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGccc                                      <  2:426812/128‑1 (MQ=255)                                                                                                                gCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACGtt                         >  2:985087/1‑140 (MQ=255)                                                                                                                gCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCtt                         >  1:666310/1‑140 (MQ=255)                                                                                                                gCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCtt                         <  2:1125237/140‑1 (MQ=255)                                                                                                                gCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCtt                         >  1:328687/1‑140 (MQ=255)                                                                                                                gCAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCtt                         <  2:306505/140‑1 (MQ=255)                                                                                                                 cAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCttt                        >  1:950149/1‑140 (MQ=255)                                                                                                                 cAAAAGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCttt                        >  2:795293/1‑140 (MQ=255)                                                                                                                    aaGAAATGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACg                     >  2:925327/1‑140 (MQ=255)                                                                                                                         aTGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAgg                >  2:1547720/1‑140 (MQ=255)                                                                                                                         aTGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCATTTCCTCACCTTTACGATAgg                >  1:963682/1‑140 (MQ=255)                                                                                                                          tGTCAAAAGAGAAGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGc               <  2:950149/140‑1 (MQ=255)                                                                                                                                     aaGGGTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt    >  2:1253640/1‑140 (MQ=255)                                                                                                                                       gggTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTcc  <  2:402433/140‑1 (MQ=255)                                                                                                                                       gggTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTcc  <  1:925327/140‑1 (MQ=255)                                                                                                                                       gggTGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTcc  <  1:795293/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCC  >  NC_000913/218743‑219016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑