Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,496,460	G→A	M272I (ATG→ATA)	lpxP →	palmitoleoyl acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,496,460	0	G	A	100.0%	333.6 / NA	92	M272I (ATG→ATA)	lpxP	palmitoleoyl acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/51);  total (41/51)

CTCCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAAT  >  NC_000913/2496328‑2496596                                                                                                                                     |                                                                                                                                         ctcCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGa                                                                                                                                   >  2:1550154/1‑140 (MQ=255)  tcCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGAt                                                                                                                                  >  1:1119056/1‑140 (MQ=255)  tcCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGAt                                                                                                                                  >  1:1491993/1‑140 (MQ=255)  tcCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGAt                                                                                                                                  >  2:1578438/1‑140 (MQ=255)  tcCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGAt                                                                                                                                  >  2:320268/1‑140 (MQ=255)  tcCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGAt                                                                                                                                  >  2:693213/1‑140 (MQ=255)   cccGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGAtt                                                                                                                                 >  1:1053536/1‑140 (MQ=255)     cGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTAt                                                                                                                               >  2:668200/1‑140 (MQ=255)     cGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTAt                                                                                                                               <  2:57634/140‑1 (MQ=255)       tctctcTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCg                                                                                                                             >  1:1136970/1‑140 (MQ=255)          ctctGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCgaga                                                                                                                          <  2:1499613/140‑1 (MQ=255)              ggCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAaga                                                                                                                      >  2:1160598/1‑140 (MQ=255)                  cAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATc                                                                                                                  >  1:840411/1‑140 (MQ=255)                    agcATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCAt                                                                                                                <  1:554977/138‑1 (MQ=255)                     ccATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATg                                                                                                               >  2:565734/1‑140 (MQ=255)                     ccATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATg                                                                                                               >  2:248475/1‑140 (MQ=255)                       aTGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcg                                                                                                             <  1:1128896/140‑1 (MQ=255)                       aTGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcg                                                                                                             <  2:1491993/140‑1 (MQ=255)                       aTGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcg                                                                                                             <  2:1306669/140‑1 (MQ=255)                        tGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcgc                                                                                                            <  2:840411/140‑1 (MQ=255)                         gTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcgcg                                                                                                           <  2:885766/140‑1 (MQ=255)                             aCCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCAcc                                                                                                       <  2:960375/140‑1 (MQ=255)                               cGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCgg                                                                                                     <  2:1557709/140‑1 (MQ=255)                               cGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCgg                                                                                                     <  2:1133556/140‑1 (MQ=255)                               cGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCgg                                                                                                     >  1:1600946/1‑140 (MQ=255)                               cGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCgg                                                                                                     >  1:267105/1‑140 (MQ=255)                                 tAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAg                                                                                                   <  2:1600946/140‑1 (MQ=255)                                  aaCGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcg                                                                                                             >  2:342477/1‑129 (MQ=255)                                  aaCGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATgcg                                                                                                             <  1:342477/129‑1 (MQ=255)                                  aaCGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCgg                                                                                                     <  2:1343119/137‑1 (MQ=255)                                  aaCGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCgg                                                                                                     >  1:1343119/1‑137 (MQ=255)                                   aCGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCa                                                                                                 >  1:7140/1‑140 (MQ=255)                                    cGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAg                                                                                                <  2:108146/140‑1 (MQ=255)                                    cGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAg                                                                                                <  2:7140/140‑1 (MQ=255)                                              aaaaGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGa                                                                                      <  2:765145/140‑1 (MQ=255)                                              aaaaGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGa                                                                                      <  2:1566647/140‑1 (MQ=255)                                                   cGGATTACAGCGGATATCGTTTGTTCATCACCCCTGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCAc                                                                                 >  2:1421127/1‑140 (MQ=255)                                                   cGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCAc                                                                                 >  2:1187202/1‑140 (MQ=255)                                                   cGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCAc                                                                                 >  1:322034/1‑140 (MQ=255)                                                   cGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCAc                                                                                 >  1:1147246/1‑140 (MQ=255)                                                     gATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGAATCCACCg                                                                               >  2:1330117/1‑140 (MQ=255)                                                      aTTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGAATCCACCGt                                                                              <  1:565734/140‑1 (MQ=255)                                                                atatCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTaaaa                                                                    <  2:322034/140‑1 (MQ=255)                                                                  atCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAAcg                                                                  <  1:941620/140‑1 (MQ=255)                                                                  atCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAAcg                                                                  <  1:1160598/140‑1 (MQ=255)                                                                  atCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAAcg                                                                  <  1:320268/140‑1 (MQ=255)                                                                    cGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAAcgcg                                                                <  2:1053536/140‑1 (MQ=255)                                                                    cGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAAcgcg                                                                <  2:506968/140‑1 (MQ=255)                                                                     gTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACgcgc                                                               <  1:693213/140‑1 (MQ=255)                                                                       ttGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGCGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGccc                                                             >  2:877165/1‑140 (MQ=255)                                                                       ttGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGccc                                                             <  2:1136970/140‑1 (MQ=255)                                                                         gTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCgg                                                           >  2:303316/1‑140 (MQ=255)                                                                          ttCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGt                                                          >  1:1427119/1‑140 (MQ=255)                                                                          ttCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGt                                                          >  1:311745/1‑140 (MQ=255)                                                                             atcaCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTggg                                                       >  1:582698/1‑140 (MQ=255)                                                                             atcaCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTggg                                                       >  2:1360399/1‑140 (MQ=255)                                                                              tcaCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGga                                                      <  1:877165/140‑1 (MQ=255)                                                                               caCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGgag                                                     >  1:150270/1‑140 (MQ=255)                                                                               caCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGgag                                                     <  2:400776/140‑1 (MQ=255)                                                                                  cccAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAt                                                  <  2:311745/140‑1 (MQ=255)                                                                                  cccAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAt                                                  <  2:1427119/140‑1 (MQ=255)                                                                                    cAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATCATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcg                                                <  1:1256888/140‑1 (MQ=255)                                                                                       agaTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcgtcg                                             >  1:1271385/1‑140 (MQ=255)                                                                                       agaTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcgtcg                                             <  2:1147246/140‑1 (MQ=255)                                                                                       agaTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcgtcg                                             <  1:1421127/140‑1 (MQ=255)                                                                                       agaTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcgtcg                                             <  1:303316/140‑1 (MQ=255)                                                                                         aTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGtt                                           <  1:1578438/140‑1 (MQ=255)                                                                                         aTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGtt                                           <  1:1187202/140‑1 (MQ=255)                                                                                            gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                        >  2:230570/1‑140 (MQ=255)                                                                                            gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                        >  1:1367483/1‑140 (MQ=255)                                                                                            gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                        >  2:68443/1‑140 (MQ=255)                                                                                             aaGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTAc                                       <  1:77133/140‑1 (MQ=255)                                                                                             aaGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTAc                                       <  2:582698/140‑1 (MQ=255)                                                                                             aaGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTAc                                       <  1:293175/140‑1 (MQ=255)                                                                                                 cTACCCGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACAttt                                   >  2:947663/1‑140 (MQ=255)                                                                                                    cccGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAAc                                >  1:1543234/1‑140 (MQ=255)                                                                                                     ccGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACa                               <  2:1119056/140‑1 (MQ=255)                                                                                                      cGACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACaa                              <  2:150270/140‑1 (MQ=255)                                                                                                       gACAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACaaa                             <  1:844644/140‑1 (MQ=255)                                                                                                        aCAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAAc                            <  1:1360399/140‑1 (MQ=255)                                                                                                         cAGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACt                           <  2:1543234/140‑1 (MQ=255)                                                                                                          aGATGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTg                          >  2:306242/1‑140 (MQ=255)                                                                                                            aTGAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGct                        <  1:947663/140‑1 (MQ=255)                                                                                                              gAAAATCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGctct                      <  2:863650/140‑1 (MQ=255)                                                                                                                 aaTCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTAc                   <  2:267105/140‑1 (MQ=255)                                                                                                                 aaTCAAGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTAc                   <  1:395432/140‑1 (MQ=255)                                                                                                                      aGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACaaa                             <  2:1173275/125‑1 (MQ=255)                                                                                                                      aGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACaaa                             >  1:1173275/1‑125 (MQ=255)                                                                                                                      aGCCGCTGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCttt              >  2:193792/1‑140 (MQ=255)                                                                                                                            tGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCcctcc        >  1:265147/1‑140 (MQ=255)                                                                                                                            tGCCTATATAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCcctcc        >  2:1209959/1‑140 (MQ=255)                                                                                                                                 atatAAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCaaa   <  1:668200/140‑1 (MQ=255)                                                                                                                                  tataAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAAt  <  1:68443/140‑1 (MQ=255)                                                                                                                                  tataAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAAt  <  1:306242/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CTCCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAAT  >  NC_000913/2496328‑2496596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑