Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,621,670	T→C	G158G (GGT→GGC)	purM →	phosphoribosylformylglycinamide cyclo‑ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,621,670	0	T	C	100.0%	235.0 / NA	66	G158G (GGT→GGC)	purM	phosphoribosylformylglycinamide cyclo‑ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/28);  total (38/28)

AAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTC  >  NC_000913/2621533‑2621805                                                                                                                                          |                                                                                                                                        aaaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGctt                                                                                                                                       <  2:730261/140‑1 (MQ=255)       gATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGctt                                                                                                                                       >  2:32473/1‑134 (MQ=255)       gATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGt                                                                                                                                 <  2:1070941/140‑1 (MQ=255)              aTACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTg                                                                                                                                    >  2:20525/1‑130 (MQ=255)              aTACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTg                                                                                                                                    <  1:20525/130‑1 (MQ=255)                  cGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAg                                                                                                                      >  2:1012438/1‑140 (MQ=255)                      tCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGaaaa                                                                                                                  >  1:727792/1‑140 (MQ=255)                      tCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGaaaa                                                                                                                  >  1:822930/1‑140 (MQ=255)                       cAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGaaaaa                                                                                                                 >  1:1347280/1‑140 (MQ=255)                           ggTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAg                                                                                                             <  2:40403/140‑1 (MQ=255)                               aTCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAat                                                                                                         >  1:160779/1‑140 (MQ=255)                               aTCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAat                                                                                                         >  1:448557/1‑140 (MQ=255)                                   gCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAatctgt                                                                                                     >  2:719926/1‑137 (MQ=255)                                   gCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAatcatc                                                                                                     >  2:771174/1‑140 (MQ=255)                                     ggCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   <  2:727792/140‑1 (MQ=255)                                     ggCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   <  1:238304/140‑1 (MQ=255)                                     ggCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   <  1:451809/140‑1 (MQ=255)                                     ggCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   <  2:822930/140‑1 (MQ=255)                                     ggCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   <  1:1255765/140‑1 (MQ=255)                                         ttGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGc                                                                                               >  1:1281223/1‑140 (MQ=255)                                         ttGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGc                                                                                               >  1:840774/1‑140 (MQ=255)                                                    tGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   >  2:821439/1‑125 (MQ=255)                                                    tGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGa                                                                                                   <  1:821439/125‑1 (MQ=255)                                                    tGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAg                                                                                    <  1:1329103/140‑1 (MQ=255)                                                      tCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCg                                                                                  >  2:1104083/1‑140 (MQ=255)                                                             tCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGa                                                                           >  1:876814/1‑140 (MQ=255)                                                                    gTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTg                                                                    >  1:164040/1‑140 (MQ=255)                                                                     ttCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGa                                                                   >  1:27674/1‑140 (MQ=255)                                                                     ttCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGa                                                                   >  1:252786/1‑140 (MQ=255)                                                                       cACTGGTGGGTGGCGAACCGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGAtt                                                                 <  2:1427384/140‑1 (MQ=255)                                                                       cACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGAtt                                                                 >  2:595340/1‑140 (MQ=255)                                                                           ggtgggtgGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCAc                                                             >  1:318863/1‑140 (MQ=255)                                                                               ggtggCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTc                                                           <  2:1137463/138‑1 (MQ=255)                                                                               ggtggCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTc                                                           >  1:1137463/1‑138 (MQ=255)                                                                               ggtggCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCgg                                                         >  1:799362/1‑140 (MQ=255)                                                                               ggtggCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCgg                                                         >  1:477611/1‑140 (MQ=255)                                                                                  ggCGAAACGGCAGGAATGCAGGGGATGTATCACGGGGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATAATCGACGGCTCTAAAGCCAGCGACGGCGATGTGCTGATTGCACTCGGTTc                                                      >  2:307900/1‑140 (MQ=255)                                                                                    cGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGa                                                                   >  2:483907/1‑125 (MQ=255)                                                                                    cGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGa                                                                   <  1:483907/125‑1 (MQ=255)                                                                                    cGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCa                                                    >  2:672948/1‑140 (MQ=255)                                                                                    cGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCa                                                    >  2:367518/1‑140 (MQ=255)                                                                                    cGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCa                                                    >  2:245119/1‑140 (MQ=255)                                                                                     gAAACGGCAGAAATGCCGGGGATGTATCGCGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAg                                                   <  2:1131094/140‑1 (MQ=255)                                                                                     gAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGTGACGGCGATGTGCTGATTGCACTCGGTTCCAg                                                   <  2:95089/140‑1 (MQ=255)                                                                                     gAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAg                                                   <  1:850109/140‑1 (MQ=255)                                                                                      aaaCGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGc                                                  >  2:338362/1‑140 (MQ=255)                                                                                           gCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTcc                                             <  1:413478/140‑1 (MQ=255)                                                                                               aaaTGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCacac                                         <  2:1347280/140‑1 (MQ=255)                                                                                                    ccGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGaa                                    <  2:252786/140‑1 (MQ=255)                                                                                                       gggATGTATCACGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACgg                                 <  1:376448/140‑1 (MQ=255)                                                                                                                 aCGGTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTg                       <  2:477611/140‑1 (MQ=255)                                                                                                                   ggTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAAc                                   <  2:759608/126‑1 (MQ=255)                                                                                                                   ggTGAGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAAc                                   >  1:759608/1‑126 (MQ=255)                                                                                                                       aGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTgcgc                 >  1:1193270/1‑140 (MQ=255)                                                                                                                       aGGATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTgcgc                 >  2:283410/1‑140 (MQ=255)                                                                                                                         gATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTTTAAAGTCAGCGACGGCGCTGTGCTGATAGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCaa               >  2:1434682/1‑140 (MQ=255)                                                                                                                         gATTACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCaa               <  2:799362/140‑1 (MQ=255)                                                                                                                            tACGATGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAAt            >  1:491776/1‑140 (MQ=255)                                                                                                                                 tGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTg       >  2:1585878/1‑140 (MQ=255)                                                                                                                                 tGTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTATAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTg       <  1:874429/140‑1 (MQ=255)                                                                                                                                  gTCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGa      >  1:121139/1‑140 (MQ=255)                                                                                                                                   tCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGaa     <  2:121139/140‑1 (MQ=255)                                                                                                                                   tCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGaa     >  1:414901/1‑140 (MQ=255)                                                                                                                                   tCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGaa     <  1:1585878/140‑1 (MQ=255)                                                                                                                                   tCGCGGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGaa     <  2:840774/140‑1 (MQ=255)                                                                                                                                    cgcgGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAg    >  2:1151185/1‑140 (MQ=255)                                                                                                                                      cgGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGATGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTCGAAGTc  >  2:1429308/1‑140 (MQ=255)                                                                                                                                      cgGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTc  <  1:283410/140‑1 (MQ=255)                                                                                                                                      cgGGCTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTc  >  2:1522987/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACGGCGATGTGCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTC  >  NC_000913/2621533‑2621805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑